乳香树胶树脂提取物在体外和体内的抗骨质疏松作用

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Nutrition Research and Practice Pub Date : 2024-06-01 Epub Date: 2024-04-17 DOI:10.4162/nrp.2024.18.3.309
Hyun Sook Lee, Jae In Jung, In-Kee Hong, YoungSun Jang, Hye-Bin Kim, Eun Ji Kim
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引用次数: 0

摘要

背景/目的:本研究评估了乳香树胶树脂乙醇提取物(FJH-UBS)对骨质疏松症的有益作用:用 FJH-UBS 处理 MC3T3-E1 成骨细胞和 RAW 264.7 破骨细胞。材料和方法:用 FJH-UBS 处理 MC3T3-E1 成骨细胞和 RAW 264.7 破骨细胞,测定其碱性磷酸酶(ALP)活性、矿化度、胶原合成、骨钙素含量、Runt 相关转录因子 2(RUNX2)和 Osterix 的表达。在 RAW 264.7 细胞中评估了肌动蛋白环结构、耐酒石酸磷酸酶(TRAP)活性和活化 T 细胞核因子细胞质 1(NFATc1)的表达。给切除卵巢的 ICR 小鼠口服 FJH-UBS 八周。结果表明:FJH-UBS 增加了小鼠的骨密度(ALT)和骨钙素(BMD),并提高了血清中骨钙素、胶原蛋白 1 N 端肽(P1NP)、骨保护素和 TRAP 5b 的水平:结果:FJH-UBS提高了MC3T3-E1成骨细胞的ALP活性、胶原蛋白、骨钙素、矿化度、RUNX2和sterix的表达,而降低了RAW 264.7破骨细胞的TRAP活性、肌动蛋白环结构和NFATc1的表达。在卵巢切除诱导的骨质疏松症小鼠中,FJH-UBS 能积极恢复卵巢切除引起的骨代谢生物标志物(BMD、骨钙素、P1NP、骨保护素和 TRAP 5b)的所有变化:结论:FJH-UBS 具有抗骨质疏松活性,在体外和体内促进成骨细胞活性,抑制破骨细胞活性,这表明 FJH-UBS 是一种潜在的治疗骨质疏松症的功能性食品配料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-osteoporotic effects of Boswellia serrata gum resin extract in vitro and in vivo.

Background/objectives: This study evaluated the beneficial effects of an ethanol extract of Boswellia serrata gum resin (FJH-UBS) in osteoporosis.

Materials/methods: MC3T3-E1 osteoblastic cells and RAW 264.7 osteoclastic cells were treated with FJH-UBS. The alkaline phosphatase (ALP) activity, mineralization, collagen synthesis, osteocalcin content, and Runt-related transcription factor 2 (RUNX2) and Osterix expression were measured in MC3T3-E1 cells. The actin ring structures, tartrate-resistant acid phosphatase (TRAP) activity, and the nuclear factor of activator T-cells, cytoplasm 1 (NFATc1) expression were evaluated in RAW 264.7 cells. Ovariectomized ICR mice were orally administered FJH-UBS for eight weeks. The bone mineral density (BMD) and the serum levels of osteocalcin, procollagen 1 N-terminal propeptide (P1NP), osteoprotegerin, and TRAP 5b were analyzed.

Results: FJH-UBS increased the ALP activity, collagen, osteocalcin, mineralization, and RUNX2 and osterix expression in MC3T3-E1 osteoblastic cells, whereas it decreased the TRAP activity, actin ring structures, and NFATc1 expression in RAW 264.7 osteoclastic cells. In ovariectomy-induced osteoporosis mice, FJH-UBS positively restored all of the changes in the bone metabolism biomarkers (BMD, osteocalcin, P1NP, osteoprotegerin, and TRAP 5b) caused by the ovariectomy.

Conclusion: FJH-UBS has anti-osteoporotic activity by promoting osteoblast activity and inhibiting osteoclast activity in vitro and in vivo, suggesting that FJH-UBS is a potential functional food ingredient for osteoporosis.

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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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