植入小鼠皮下组织的生物陶瓷和环氧树脂牙髓封闭剂的炎症反应:体内研究。

IF 2 3区 工程技术 Q2 ANATOMY & MORPHOLOGY
Vanessa Valente Elias, Ricardo Barbosa Lima, Marília Pacífico Lucisano, Lisa Danielly Curcino Araujo, Carolina Maschietto Pucinelli, Paulo Nelson-Filho, Raquel Assed Bezerra da Silva, Léa Assed Bezerra da Silva
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引用次数: 0

摘要

评估小鼠皮下组织对 BioRoot™ RCS (BR) 和 AH Plus Jet (AHPJ) 密封剂的炎症组织反应。假设对 BR 的炎症组织反应会比对 AHPJ 的反应轻微。使用同种异体小鼠进行了一项体内研究。在标准化手术过程中植入了密封剂。7、21 和 63 天后,通过显微镜分析和标本周围反应组织的 von Kossa 反应评估炎症反应。为了进行比较,除了不使用封闭剂的阴性对照组外,还使用了氧化锌和丁香酚封闭剂(ZOE)作为阳性对照组(每组/每期 n = 10)。所有统计分析的显著性水平均为 5%。所有牙髓封闭剂在 7 天后都会引发炎症组织反应。与 AHPJ 相比,BR 的炎症细胞数更多,纤维囊更厚,但两者的炎症反应都比 ZOE 轻(p<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Inflammatory response to bioceramic and epoxy resin-based endodontic sealers implanted in mice subcutaneous tissue: An in vivo study

Inflammatory response to bioceramic and epoxy resin-based endodontic sealers implanted in mice subcutaneous tissue: An in vivo study

To evaluate the inflammatory tissue response to BioRoot™ RCS (BR) and AH Plus Jet (AHPJ) sealers implanted in mice subcutaneous tissue. It was hypothesized that the inflammatory tissue response to BR would be milder than to AHPJ. An in vivo study was carried out using isogenic mice. The sealers were implanted during standardized surgical procedures. The inflammatory response was evaluated by microscopic analysis and von Kossa reaction in the reactionary tissue around the specimens after 7, 21, and 63 days. For comparisons, a zinc oxide and eugenol sealer (ZOE) was used as a positive control, in addition to a negative control without a sealer (n = 10 per group/period). All statistical analyses considered a significance level of 5%. All endodontic sealers triggered an inflammatory tissue response after 7 days. BR had a higher inflammatory cell count and a thicker fibrous capsule when compared with AHPJ, but both were less inflammatory than ZOE (p < .001). After 21 days, BR continued to trigger an intense inflammatory tissue response, higher in both microscopic parameters compared to AHPJ, and a thicker fibrous capsule than ZOE (p < .001). After 63 days, the inflammatory tissue response decreased in BR, matching the fibrous capsule thickness with AHPJ and ZOE. BR promoted intense calcium precipitation in all study periods. After 63 days, AHPJ and BR sealers were more biocompatible to subcutaneous mice tissue, but AHPJ present better early inflammatory response, as well as BR showed potential bioactivity.

Research Highlights

  • The inflammatory tissue response triggered by a bioceramic endodontic sealer (BR) was not milder than that triggered by an epoxy-resin based endodontic sealer (AHPJ) during the first 3 weeks, considering the microscopic analysis of the reactionary tissue.
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来源期刊
Microscopy Research and Technique
Microscopy Research and Technique 医学-解剖学与形态学
CiteScore
5.30
自引率
20.00%
发文量
233
审稿时长
4.7 months
期刊介绍: Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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