育雏鸡和产蛋茶花鸡的卵巢转录组分析。

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Poultry Science Pub Date : 2024-06-08 eCollection Date: 2024-01-01 DOI:10.2141/jpsa.2024018
Yanli Du, Huang Wei, Meiquan Li, Xiao Wang, Jinshan Ran, Jing Wang, Cuilian Dong, Na Zhu
{"title":"育雏鸡和产蛋茶花鸡的卵巢转录组分析。","authors":"Yanli Du, Huang Wei, Meiquan Li, Xiao Wang, Jinshan Ran, Jing Wang, Cuilian Dong, Na Zhu","doi":"10.2141/jpsa.2024018","DOIUrl":null,"url":null,"abstract":"<p><p>Broodiness in egg-laying hens (EHs) leads to ovarian atrophy, resulting in reduced egg-laying performance. However, the ovarian regulatory mechanisms in broody hens (BCs) remain elusive. Therefore, ovaries were removed from 300-day-old BCs and EHs for RNA sequencing. Ovarian morphology and histological characteristics of the BC and EH groups were compared and analyzed. The EH group had significantly more hierarchical follicles (HFs) and small yellow follicles (SYFs) than that of the BC group. Although several secondary follicles (SFs) and primary follicles were observed in the ovaries of the EH group, only a few SFs were observed in the ovaries of the BC group. Subsequently, RNA-sequencing analysis was conducted to determine the ovarian expression profiles of the two groups. Transcriptome sequencing identified 259 differentially expressed genes (DEGs) between the BC and EH groups. Of the 259 DEGs, 136 were upregulated and 123 were downregulated. The DEGs were mapped to 22 gene ontology terms and 4 Kyoto Encyclopedia of Genes and Genomes pathways for ovarian tissue. The analysis showed that matrix metalloproteinases 11/13 (MMP11/MMP13) were enriched in the extracellular matrix. The extracellular matrix mediated by MMP13 is affected by follicle-stimulating hormone, prolactin, and estrogen, which are critical signaling pathways that may affect ovarian follicle development to regulate the large yellow follicle reserve process and the ovulation cycle of broody Chahua chickens. These findings indicate that understanding differences in gene expression between the ovarian tissues of BCs and EHs could serve as a valuable reference point for enhancing egg-laying performance in Chahua chickens.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024018"},"PeriodicalIF":1.8000,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156485/pdf/","citationCount":"0","resultStr":"{\"title\":\"Ovary Transcriptome Profiling in Broody and Egg-laying Chahua Chickens.\",\"authors\":\"Yanli Du, Huang Wei, Meiquan Li, Xiao Wang, Jinshan Ran, Jing Wang, Cuilian Dong, Na Zhu\",\"doi\":\"10.2141/jpsa.2024018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Broodiness in egg-laying hens (EHs) leads to ovarian atrophy, resulting in reduced egg-laying performance. However, the ovarian regulatory mechanisms in broody hens (BCs) remain elusive. Therefore, ovaries were removed from 300-day-old BCs and EHs for RNA sequencing. Ovarian morphology and histological characteristics of the BC and EH groups were compared and analyzed. The EH group had significantly more hierarchical follicles (HFs) and small yellow follicles (SYFs) than that of the BC group. Although several secondary follicles (SFs) and primary follicles were observed in the ovaries of the EH group, only a few SFs were observed in the ovaries of the BC group. Subsequently, RNA-sequencing analysis was conducted to determine the ovarian expression profiles of the two groups. Transcriptome sequencing identified 259 differentially expressed genes (DEGs) between the BC and EH groups. Of the 259 DEGs, 136 were upregulated and 123 were downregulated. The DEGs were mapped to 22 gene ontology terms and 4 Kyoto Encyclopedia of Genes and Genomes pathways for ovarian tissue. The analysis showed that matrix metalloproteinases 11/13 (MMP11/MMP13) were enriched in the extracellular matrix. The extracellular matrix mediated by MMP13 is affected by follicle-stimulating hormone, prolactin, and estrogen, which are critical signaling pathways that may affect ovarian follicle development to regulate the large yellow follicle reserve process and the ovulation cycle of broody Chahua chickens. These findings indicate that understanding differences in gene expression between the ovarian tissues of BCs and EHs could serve as a valuable reference point for enhancing egg-laying performance in Chahua chickens.</p>\",\"PeriodicalId\":16883,\"journal\":{\"name\":\"Journal of Poultry Science\",\"volume\":\"61 \",\"pages\":\"2024018\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-06-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156485/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2141/jpsa.2024018\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2141/jpsa.2024018","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

摘要

产蛋鸡(EHs)的育雏会导致卵巢萎缩,从而降低产蛋性能。然而,育雏母鸡(BCs)的卵巢调节机制仍然难以捉摸。因此,研究人员从300日龄的育雏鸡和EHs中取出卵巢进行RNA测序。对BC组和EH组的卵巢形态和组织学特征进行了比较和分析。EH组的分层卵泡(HFs)和小黄卵泡(SYFs)明显多于BC组。虽然在EH组的卵巢中观察到了几个次级卵泡(SFs)和初级卵泡,但在BC组的卵巢中只观察到了几个SFs。随后,进行了 RNA 序列分析,以确定两组患者的卵巢表达谱。转录组测序在 BC 组和 EH 组之间发现了 259 个差异表达基因(DEG)。在这 259 个 DEGs 中,136 个基因上调,123 个基因下调。DEGs被映射到卵巢组织的22个基因本体术语和4个京都基因和基因组百科全书通路。分析表明,基质金属蛋白酶11/13(MMP11/MMP13)在细胞外基质中富集。由MMP13介导的细胞外基质受卵泡刺激素、催乳素和雌激素的影响,而卵泡刺激素、催乳素和雌激素是可能影响卵巢卵泡发育的关键信号通路,可调节肉用茶花鸡的大黄卵泡储备过程和排卵周期。这些研究结果表明,了解BC和EH卵巢组织基因表达的差异可为提高茶花鸡的产蛋性能提供有价值的参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ovary Transcriptome Profiling in Broody and Egg-laying Chahua Chickens.

Broodiness in egg-laying hens (EHs) leads to ovarian atrophy, resulting in reduced egg-laying performance. However, the ovarian regulatory mechanisms in broody hens (BCs) remain elusive. Therefore, ovaries were removed from 300-day-old BCs and EHs for RNA sequencing. Ovarian morphology and histological characteristics of the BC and EH groups were compared and analyzed. The EH group had significantly more hierarchical follicles (HFs) and small yellow follicles (SYFs) than that of the BC group. Although several secondary follicles (SFs) and primary follicles were observed in the ovaries of the EH group, only a few SFs were observed in the ovaries of the BC group. Subsequently, RNA-sequencing analysis was conducted to determine the ovarian expression profiles of the two groups. Transcriptome sequencing identified 259 differentially expressed genes (DEGs) between the BC and EH groups. Of the 259 DEGs, 136 were upregulated and 123 were downregulated. The DEGs were mapped to 22 gene ontology terms and 4 Kyoto Encyclopedia of Genes and Genomes pathways for ovarian tissue. The analysis showed that matrix metalloproteinases 11/13 (MMP11/MMP13) were enriched in the extracellular matrix. The extracellular matrix mediated by MMP13 is affected by follicle-stimulating hormone, prolactin, and estrogen, which are critical signaling pathways that may affect ovarian follicle development to regulate the large yellow follicle reserve process and the ovulation cycle of broody Chahua chickens. These findings indicate that understanding differences in gene expression between the ovarian tissues of BCs and EHs could serve as a valuable reference point for enhancing egg-laying performance in Chahua chickens.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信