用于诊断肾病的尿液荚膜衍生移行体定量分析

IF 15.5 1区 医学 Q1 CELL BIOLOGY
Rong Yang, Heng Zhang, Si Chen, Kaibin Lou, Meng Zhou, Mingchao Zhang, Rui Lu, Chunxia Zheng, Limin Li, Qihan Chen, Zhihong Liu, Ke Zen, Yanggang Yuan, Hongwei Liang
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引用次数: 0

摘要

迁移体是最近发现的一类细胞外微囊泡,直径范围在 500 纳米到 3000 纳米之间。它们由迁移的细胞释放出来,携带着多种多样的 RNA 和蛋白质。迁移体很容易在血清和尿液等体液中被识别出来,因此是通过液体活检进行疾病诊断的重要非侵入性来源。在这项研究中,我们采用小麦胚芽凝集素(WGA)包被磁珠和流式细胞术(简称 WBFC),介绍了一种捕获和定量评估迁移体的简便有效的方法。随后,我们利用流式细胞术检测了荚膜细胞损伤的肾病(KD)患者和健康志愿者尿液中的移行体水平。结果显示,与健康志愿者相比,荚膜细胞损伤的肾病患者尿液中荚膜细胞衍生的migrasome浓度大幅增加。值得注意的是,研究发现尿液中的荚膜细胞衍生移行体表达大量磷脂酶 A2 受体(PLA2R)蛋白。这些移行小体中存在的 PLA2R 蛋白有望作为一种天然抗原,用于定量检测膜性肾病患者血清中针对 PLA2R 的自身抗体。因此,我们的研究不仅开创了分离和量化移行小体的新技术,还强调了尿液中的移行小体作为早期诊断荚膜细胞损伤的KD生物标志物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease

Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease

Migrasomes represent a recently uncovered category of extracellular microvesicles, spanning a diameter range of 500 to 3000 nm. They are emitted by migrating cells and harbour a diverse array of RNAs and proteins. Migrasomes can be readily identified in bodily fluids like serum and urine, rendering them a valuable non-invasive source for disease diagnosis through liquid biopsy. In this investigation, we introduce a streamlined and effective approach for the capture and quantitative assessment of migrasomes, employing wheat germ agglutinin (WGA)-coated magnetic beads and flow cytometry (referred to as WBFC). Subsequently, we examined the levels of migrasomes in the urine of kidney disease (KD) patients with podocyte injury and healthy volunteers using WBFC. The outcomes unveiled a substantial increase in urinary podocyte-derived migrasome concentrations among individuals with KD with podocyte injury compared to the healthy counterparts. Notably, the urinary podocyte-derived migrasomes were found to express an abundant quantity of phospholipase A2 receptor (PLA2R) proteins. The presence of PLA2R proteins in these migrasomes holds promise for serving as a natural antigen for the quantification of autoantibodies against PLA2R in the serum of patients afflicted by membranous nephropathy. Consequently, our study not only pioneers a novel technique for the isolation and quantification of migrasomes but also underscores the potential of urinary migrasomes as a promising biomarker for the early diagnosis of KD with podocyte injury.

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来源期刊
Journal of Extracellular Vesicles
Journal of Extracellular Vesicles Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
27.30
自引率
4.40%
发文量
115
审稿时长
12 weeks
期刊介绍: The Journal of Extracellular Vesicles is an open access research publication that focuses on extracellular vesicles, including microvesicles, exosomes, ectosomes, and apoptotic bodies. It serves as the official journal of the International Society for Extracellular Vesicles and aims to facilitate the exchange of data, ideas, and information pertaining to the chemistry, biology, and applications of extracellular vesicles. The journal covers various aspects such as the cellular and molecular mechanisms of extracellular vesicles biogenesis, technological advancements in their isolation, quantification, and characterization, the role and function of extracellular vesicles in biology, stem cell-derived extracellular vesicles and their biology, as well as the application of extracellular vesicles for pharmacological, immunological, or genetic therapies. The Journal of Extracellular Vesicles is widely recognized and indexed by numerous services, including Biological Abstracts, BIOSIS Previews, Chemical Abstracts Service (CAS), Current Contents/Life Sciences, Directory of Open Access Journals (DOAJ), Journal Citation Reports/Science Edition, Google Scholar, ProQuest Natural Science Collection, ProQuest SciTech Collection, SciTech Premium Collection, PubMed Central/PubMed, Science Citation Index Expanded, ScienceOpen, and Scopus.
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