Koeun Hwang , Serhat Al , Robert E. Campbell , Kathleen Glass , Kurt D. Vogel , James R. Claus
{"title":"在绵羊和山羊中建立实验感染模型,以评估沙门氏菌在深层组织淋巴结中的定植情况以及用噬菌体冲洗山羊胴体血管后的情况。","authors":"Koeun Hwang , Serhat Al , Robert E. Campbell , Kathleen Glass , Kurt D. Vogel , James R. Claus","doi":"10.1016/j.jfp.2024.100312","DOIUrl":null,"url":null,"abstract":"<div><p>An animal infection model was evaluated on sheep and goats to confirm which species infected with <em>Salmonella enterica</em> serovar Enteritidis C StR (SE13) would provide a consistent and high frequency of <em>Salmonella</em> colonization in lymph nodes (LNs) without causing undue animal morbidity. Sheep and goats (<em>n</em> = 5) were intradermally inoculated with <em>Salmonella</em>, postincubated for 7 days, and euthanized. Superficial cervical, medial iliac, subiliac, mammary, and popliteal LNs were excised from each carcass. Goat LNs had approximately 53% greater <em>Salmonella</em> level compared to sheep. Also, <em>Salmonella</em> was inconsistently recovered from the sheep LNs. Thus, goats were selected to determine the ability of carcass vascular rinsing (with and without bacteriophages) to reduce <em>Salmonella</em> in infected LNs. Goats with similar characteristics were grouped together before being randomly assigned to 3 postharvest treatments; control (CN, not vascularly rinsed; <em>n</em> = 10), vascularly rinsed with a standard Rinse & Chill® solution (RC; 98.5% water and a blend of saccharides and phosphates; <em>n</em> = 10), or vascularly rinsed with a standard Rinse & Chill® solution plus the addition of bacteriophages (BP; <em>n</em> = 10). Rinse & Chill® system was able to successfully deliver a mean 7.0 log PFU/g to the <em>S.</em> Enteritidis-infected LNs (mean 3.5 log CFU/g). However, neither Rinse & Chill® without bacteriophages nor with bacteriophages caused <em>Salmonella</em> reduction (<em>P</em> > 0.05) compared to the nonrinsed goat carcasses.</p></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0362028X24000966/pdfft?md5=88d5b4c07e069039aeb7fb9cb1af8ec5&pid=1-s2.0-S0362028X24000966-main.pdf","citationCount":"0","resultStr":"{\"title\":\"An Experimental Infection Model in Sheep and Goats to Evaluate Salmonella Colonization in Deep Tissue Lymph Nodes and after Carcass Vascular Rinsing with Bacteriophages in Goats\",\"authors\":\"Koeun Hwang , Serhat Al , Robert E. Campbell , Kathleen Glass , Kurt D. Vogel , James R. Claus\",\"doi\":\"10.1016/j.jfp.2024.100312\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An animal infection model was evaluated on sheep and goats to confirm which species infected with <em>Salmonella enterica</em> serovar Enteritidis C StR (SE13) would provide a consistent and high frequency of <em>Salmonella</em> colonization in lymph nodes (LNs) without causing undue animal morbidity. Sheep and goats (<em>n</em> = 5) were intradermally inoculated with <em>Salmonella</em>, postincubated for 7 days, and euthanized. Superficial cervical, medial iliac, subiliac, mammary, and popliteal LNs were excised from each carcass. Goat LNs had approximately 53% greater <em>Salmonella</em> level compared to sheep. Also, <em>Salmonella</em> was inconsistently recovered from the sheep LNs. Thus, goats were selected to determine the ability of carcass vascular rinsing (with and without bacteriophages) to reduce <em>Salmonella</em> in infected LNs. Goats with similar characteristics were grouped together before being randomly assigned to 3 postharvest treatments; control (CN, not vascularly rinsed; <em>n</em> = 10), vascularly rinsed with a standard Rinse & Chill® solution (RC; 98.5% water and a blend of saccharides and phosphates; <em>n</em> = 10), or vascularly rinsed with a standard Rinse & Chill® solution plus the addition of bacteriophages (BP; <em>n</em> = 10). Rinse & Chill® system was able to successfully deliver a mean 7.0 log PFU/g to the <em>S.</em> Enteritidis-infected LNs (mean 3.5 log CFU/g). 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An Experimental Infection Model in Sheep and Goats to Evaluate Salmonella Colonization in Deep Tissue Lymph Nodes and after Carcass Vascular Rinsing with Bacteriophages in Goats
An animal infection model was evaluated on sheep and goats to confirm which species infected with Salmonella enterica serovar Enteritidis C StR (SE13) would provide a consistent and high frequency of Salmonella colonization in lymph nodes (LNs) without causing undue animal morbidity. Sheep and goats (n = 5) were intradermally inoculated with Salmonella, postincubated for 7 days, and euthanized. Superficial cervical, medial iliac, subiliac, mammary, and popliteal LNs were excised from each carcass. Goat LNs had approximately 53% greater Salmonella level compared to sheep. Also, Salmonella was inconsistently recovered from the sheep LNs. Thus, goats were selected to determine the ability of carcass vascular rinsing (with and without bacteriophages) to reduce Salmonella in infected LNs. Goats with similar characteristics were grouped together before being randomly assigned to 3 postharvest treatments; control (CN, not vascularly rinsed; n = 10), vascularly rinsed with a standard Rinse & Chill® solution (RC; 98.5% water and a blend of saccharides and phosphates; n = 10), or vascularly rinsed with a standard Rinse & Chill® solution plus the addition of bacteriophages (BP; n = 10). Rinse & Chill® system was able to successfully deliver a mean 7.0 log PFU/g to the S. Enteritidis-infected LNs (mean 3.5 log CFU/g). However, neither Rinse & Chill® without bacteriophages nor with bacteriophages caused Salmonella reduction (P > 0.05) compared to the nonrinsed goat carcasses.
期刊介绍:
The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with:
Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain;
Microbiological food quality and traditional/novel methods to assay microbiological food quality;
Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation;
Food fermentations and food-related probiotics;
Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers;
Risk assessments for food-related hazards;
Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods;
Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.