利用原子力显微镜观察低矿物质温泉水对皮肤屏障机械特性的影响

IF 3.5 3区 医学 Q1 DERMATOLOGY
C. Mias, A. Stennevin, G. Doat, A. Catté, J. Chlasta, S. Bessou-Touya, H. Duplan
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引用次数: 0

摘要

涂抹在皮肤表面的温泉水(TSW)的矿物质含量会直接影响皮肤屏障。事实上,我们之前的研究表明,与富含矿物质的温泉水(MR-TSW)相比,矿物质含量低的雅漾温泉水(ATSW)能保护角质层不脱水,并保持皮肤表面的超微结构。虽然许多温泉水都被认为对皮肤有好处,但人们对它们在纳米尺度上对皮肤屏障生物力学的局部和特定影响知之甚少。本研究的目的是利用原子力显微镜(AFM)比较 ATSW 和 MR-TSW 在平衡状态下对皮肤生物力学屏障特性的影响。使用原子力显微镜可以在两种 TSW 使用三次后获得皮肤表面的精确纳米力学图谱。这提供了有关皮肤地形轮廓和弹性的具体信息。与未处理的样本相比,使用 MR-TSW 后,皮肤样本的地形轮廓显示出皮肤层的特定压实,其特点是外部皮肤层总数增加。相比之下,ATSW 没有改变皮肤的地形轮廓。通过高分辨率力/体积采集来捕捉弹性模量,结果显示弹性模量与皮肤刚度直接相关。与未经处理的皮肤相比,使用 MR-TSW 后弹性模量明显增加。相比之下,应用 ATSW 并未增加弹性模量。这些数据表明,MR-TSW 的应用通过增加皮肤表层的压实度和皮肤硬度,明显改善了皮肤的屏障特性。相比之下,ATSW 既不会改变皮肤样本的地形轮廓,也不会引起角质层的机械应力,这表明它不会破坏与皮肤表面完整性相关的生物物理特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of a low-mineralized thermal spring water on skin barrier mechanical properties using atomic force microscopy

The mineral content of thermal spring water (TSW) applied to the skin surface can directly influence the skin barrier. Indeed, our previous study showed that Avène TSW (ATSW), a low mineral content thermal spring water, protects the stratum corneum from dehydration compared to a mineral-rich TSW (MR-TSW) and maintains skin surface ultrastructure. While many TSWs have been recognized to have beneficial effects on skin, little is known about their localized and specific effects on skin barrier biomechanics at the nanometric scale. The aim of this study was to compare the effects of ATSW with a reference, MR-TSW, on the biomechanical barrier properties of the skin under homeostasis conditions using atomic force microscopy (AFM). AFM was used to obtain a precise nanomechanical mapping of the skin surface after three applications of both TSW. This provides specific information on the skin topographical profile and elasticity. The topographic profile of skin samples showed a specific compaction of the skin layers after application of MR-TSW, characterized by an increase of the total number of external skin layers, compared to non-treated samples. By contrast, ATSW did not modify the skin topographic profile. High-resolution force/volume acquisitions to capture the elastic modulus showed that it was directly correlated with skin rigidity. The elastic modulus strongly and significantly increased after MR-TSW application compared to non-treated skin. By contrast, applications of ATSW did not increase elastic modulus. These data demonstrate that applications of MR-TSW significantly modified skin barrier properties by increasing skin surface layer compaction and skin rigidity. By contrast, ATSW did not modify the topographical profile of skin explants nor induce mechanical stress at the level of the stratum corneum, indicating it does not disrupt the biophysical properties linked to skin surface integrity.

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来源期刊
Experimental Dermatology
Experimental Dermatology 医学-皮肤病学
CiteScore
6.70
自引率
5.60%
发文量
201
审稿时长
2 months
期刊介绍: Experimental Dermatology provides a vehicle for the rapid publication of innovative and definitive reports, letters to the editor and review articles covering all aspects of experimental dermatology. Preference is given to papers of immediate importance to other investigators, either by virtue of their new methodology, experimental data or new ideas. The essential criteria for publication are clarity, experimental soundness and novelty. Letters to the editor related to published reports may also be accepted, provided that they are short and scientifically relevant to the reports mentioned, in order to provide a continuing forum for discussion. Review articles represent a state-of-the-art overview and are invited by the editors.
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