不同哺乳期的母乳对体外伤口愈合的影响

IF 2.1 3区 医学 Q2 OBSTETRICS & GYNECOLOGY
Breastfeeding Medicine Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI:10.1089/bfm.2024.0099
Cigdem Aydin Acar, Suray Pehlivanoglu, Sukriye Yesilot, Halil Ibrahim Tasdemir
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引用次数: 0

摘要

目的:伤口愈合是一个复杂而动态的过程,对恢复组织的完整性和平衡至关重要。人们认为,母乳所含的成分对伤口愈合过程有积极的促进作用。本研究旨在比较母乳在不同哺乳阶段对伤口愈合过程的影响,并评估其潜在机制。材料与方法:通过 L929 成纤维细胞体外划痕试验测定不同哺乳期母乳(初乳、过渡乳和成熟乳)对伤口愈合的影响。2,2-二苯基-1-苦基肼(DPPH)、总氧化剂和抗氧化能力被用来确认抗氧化作用。用酶联免疫吸附法阐明了母乳对 L929 细胞中 netrin-1 水平的影响。结果显示不同哺乳期的母乳都能促进伤口愈合。对照组的伤口愈合率为 48.7%,而成熟乳组的伤口愈合率最高,为 81.6%(p:0.0002)。根据泌乳阶段的不同,初乳、过渡乳和成熟乳用 DPPH 清除自由基的能力分别为 49.69%、60.64% 和 80.85%。用酶联免疫吸附法测定,对照组的净素-1 水平为 490.1 ± 6.5 pg/mL,而成熟乳的净素-1 水平最低,为 376.6 ± 4.5 pg/mL(P:0.0003)。结论母乳,尤其是成熟乳,可通过抑制 netrin-1 水平和清除自由基促进 L929 细胞的伤口愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Breast Milk from Different Lactation Stages on in Vitro Wound Healing.

Objective: Wound healing is a complex and dynamic process essential for restoring tissue integrity and homeostasis. It is thought that breast milk contributes positively to the wound healing process, thanks to the components it contains. The aim of this study is to compare the effects of breast milk on the wound healing process at different lactation stages and to evaluate the underlying mechanism(s). Materials and Methods: The effects of breast milk from different lactation stages (colostrum, transitional, and mature milk) on wound healing were determined by in vitro scratch assay in L929 fibroblast cells. 2,2-Diphenyl-1-picrylhydrazyl (DPPH), total oxidant, and antioxidant capacity were used to confirm antioxidant effects. The effect of breast milk on netrin-1 levels in L929 cells was elucidated by ELISA. Results: Breast milk at different lactation stages promoted wound healing. While the wound closure percentage was determined as 48.7% in the control group, this rate was determined to be the highest at 81.6% in the mature milk group (p:0.0002). The free radical scavenging capacity of colostrum, transitional, and mature milk with DPPH was determined as 49.69%, 60.64%, and 80.85%, respectively, depending on the lactation stages. Netrin-1 levels detected by ELISA were determined as 490.1 ± 6.5 pg/mL in the control group, while the lowest level was determined as 376.6 ± 4.5 pg/mL in mature milk (p:0.0003). Conclusions: Breast milk, especially mature milk, promoted wound healing on L929 cells by suppressing netrin-1 levels and scavenging free radicals.

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来源期刊
Breastfeeding Medicine
Breastfeeding Medicine OBSTETRICS & GYNECOLOGY-PEDIATRICS
CiteScore
4.20
自引率
11.10%
发文量
130
审稿时长
6-12 weeks
期刊介绍: Breastfeeding Medicine provides unparalleled peer-reviewed research, protocols, and clinical applications to ensure optimal care for mother and infant. The Journal answers the growing demand for evidence-based research and explores the immediate and long-term outcomes of breastfeeding, including its epidemiologic, physiologic, and psychological benefits. It is the exclusive source of the Academy of Breastfeeding Medicine protocols. Breastfeeding Medicine coverage includes: Breastfeeding recommendations and protocols Health consequences of artificial feeding Physiology of lactation and biochemistry of breast milk Optimal nutrition for the breastfeeding mother Breastfeeding indications and contraindications Managing breastfeeding discomfort, pain, and other complications Breastfeeding the premature or sick infant Breastfeeding in the chronically ill mother Management of the breastfeeding mother on medication Infectious disease transmission through breast milk and breastfeeding The collection and storage of human milk and human milk banking Measuring the impact of being a “baby-friendly” hospital Cultural competence and cultural sensitivity International public health issues including social and economic issues.
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