通过调节5-HT4/cAMP/PKA/AQP4信号通路促进STC模型小鼠结肠转运功能的作用和机制

IF 4.8 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhuoli Yu , Lalai Zikela , Dingli Wang , Xuezhu Wang , Huilin Zhu , Songtao Li , Qiang Han
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引用次数: 0

摘要

据报道,大叶香榧油(Torreya grandis,T. grandis)可减轻慢传输性便秘(STC)的症状。然而,大叶香榧油中的一种独特脂肪酸--香榧酸(SA)对STC病理进展的影响仍不清楚。本研究旨在评估SA对STC的影响并揭示其潜在机制。通过给 Balb/c 小鼠喂食洛哌丁胺,建立了 STC 模型。干预2周后,SA明显改善了STC引起的体重下降和肠蠕动减弱,同时提高了血浆指数,减少了结肠病理损伤。SA能有效逆转STC诱导的5-HT4/cAMP/PKA/AQP4信号通路基因的减少和表达。此外,16S rRNA 分析表明,SA 可降低固缩菌与类杆菌(F/B)的比例,增加有益菌(如 Akkermansia)的数量,从而缓解 STC 引起的肠道微生物群失衡。总之,SA干预缓解了STC小鼠的结肠功能障碍。激活 SA 介导的 5-HT4/cAMP/PKA/AQP4 信号通路可能是 STC 治疗的潜在靶点。这些研究结果表明,SA有望成为治疗STC的一种选择,并有可能扩展到其他相关的肠道疾病,以供进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects and mechanisms of sciadonic acid on colonic transit function through regulating 5-HT4/cAMP/PKA/AQP4 signaling pathway in STC model mice

Effects and mechanisms of sciadonic acid on colonic transit function through regulating 5-HT4/cAMP/PKA/AQP4 signaling pathway in STC model mice

Torreya grandis (T. grandis) oil has been reported to alleviate symptoms of slow transit constipation (STC). However, the impact of sciadonic acid (SA), a distinctive fatty acid found in T. grandis oil, on the pathological progression of STC remains unclear. This study aimed to evaluate the effect of SA on STC and uncover the underlying mechanisms. The STC model was established by feeding Balb/c mice with loperamide. After 2 weeks of intervention, SA significantly improved weight loss and intestinal motility decline induced by STC, along with enhancing plasma indices and reducing colon pathological damage. SA effectively reversed the STC-induced decrease in the 5-HT4/cAMP/PKA/AQP4 signaling pathway genes and expression. Furthermore, 16S rRNA analysis demonstrated that SA mitigated the imbalance of the intestinal microbiota induced by STC, by reducing the ratio of Firmicutes to Bacteroidetes (F/B) and increasing the abundance of beneficial bacteria such as Akkermansia. In conclusion, SA intervention alleviated colonic dysfunction in STC mice. The activation of the SA-mediated 5-HT4/cAMP/PKA/AQP4 signaling pathway may serve as a potential target for STC treatment. These findings suggest that SA holds promise as a treatment option for STC and could potentially be extended to other related gut diseases for further investigation.

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来源期刊
Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry 医学-生化与分子生物学
CiteScore
9.50
自引率
3.60%
发文量
237
审稿时长
68 days
期刊介绍: Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
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