病毒表面显示的嵌合免疫球蛋白 Fc 结构域有助于抗原递呈细胞的吸收。

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sayuri Seki , Prince Kofi Parbie , Hiroyuki Yamamoto , Tetsuro Matano
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引用次数: 0

摘要

抗原递呈细胞(APCs)通过连接先天性免疫反应和适应性免疫反应,在病毒感染控制中发挥着重要作用。巨噬细胞和树突状细胞(DCs)拥有多种表面受体来识别/内化抗原,抗体结合可通过抗体片段可结晶(Fc)结构域与 Fc 受体的相互作用增强这些 APCs 对病原体的吞噬作用,从而在某些情况下唤起对病原体的深度控制。在这里,我们研究了直接位于逆转录病毒病毒/类病毒颗粒(VLP)表面的 Fc 结构域的吞噬增强潜力。我们生成了一种编码与逆转录病毒包膜蛋白跨膜区(TM)融合的小鼠 Fc 片段的表达载体,从而在转染细胞表面表达 Fc-TM 融合蛋白,并在共转染时产生包含嵌合 Fc 的病毒。将显示 Fc 的猿猴免疫缺陷病毒(SIV)与小鼠 J774 巨噬细胞和骨髓源性直流细胞孵育,可获得 Fc 受体依赖性增强的摄取,并可通过成像细胞仪观察到。另一种基于小鼠白血病病毒(MLV)骨架的 Fc 显性 VLP 的制备方法加载了流感病毒血凝素(HA)抗原,结果增强了巨噬细胞对 HA 的内吸收,证明了该设计的抗原兼容性。结果表明,Fc-TM 融合分子可显示在某些病毒/VLP 上,并可用作分子佐剂,促进 APC 抗原的吸收。(200字)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Virion-surface display of a chimeric immunoglobulin Fc domain facilitating uptake by antigen-presenting cells

Antigen-presenting cells (APCs) play an important role in virus infection control by bridging innate and adaptive immune responses. Macrophages and dendritic cells (DCs) possess various surface receptors to recognize/internalize antigens, and antibody binding can enhance pathogen-opsonizing uptake by these APCs via interaction of antibody fragment crystallizable (Fc) domains with Fc receptors, evoking profound pathogen control in certain settings. Here, we examined phagocytosis-enhancing potential of Fc domains directly oriented on a retroviral virion/virus-like particle (VLP) surface. We generated an expression vector coding a murine Fc fragment fused to the transmembrane region (TM) of a retroviral envelope protein, deriving expression of the Fc-TM fusion protein on the transfected cell surface and production of virions incorporating the chimeric Fc upon co-transfection. Incubation of Fc-displaying simian immunodeficiency virus (SIV) with murine J774 macrophages and bone marrow-derived DCs derived Fc receptor-dependent enhanced uptake, being visualized by imaging cytometry. Alternative preparation of a murine leukemia virus (MLV) backbone-based Fc-displaying VLP loading an influenza virus hemagglutinin (HA) antigen resulted in enhanced HA internalization by macrophages, stating antigen compatibility of the design. Results show that the Fc-TM fusion molecule can be displayed on certain viruses/VLPs and may be utilized as a molecular adjuvant to facilitate APC antigen uptake.

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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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