{"title":"打破 Rhodomyrtus tomentosa (Aiton) Hassk.种子休眠以提高种子发芽率的有效技术","authors":"Suttiwan Wunnoo , Tewee Maneerat , Ladawan Lerslerwong , Supayang Piyawan Voravuthikunchai , Pattamavadee Kunwanlee","doi":"10.1016/j.jarmap.2024.100558","DOIUrl":null,"url":null,"abstract":"<div><p><em>Rhodomyrtus tomentosa</em> is a valuable plant for medical and pharmaceutical uses. The plant reproduces through seeds; however, the seeds become dormant, resulting in low germination rates. The industrial demand for this plant is expanding, making sustainable propagation a major challenge. The present study aims to evaluate different techniques for breaking the dormancy of <em>R. tomentosa</em> seeds. A randomized design was used to evaluate different techniques for breaking the dormancy of <em>R. tomentosa</em> seeds, including de-operculum, chemical scarifications, and exogenous gibberellic acid (GA<sub>3</sub>), in both laboratory conditions at ambient temperature (25 ± 2 °C) and field conditions. The characteristics of <em>R. tomentosa</em> fruit and seeds were assessed. The average mass, width, and length of fruits were 1.90 g, 13.78 g, and 15.27 g, respectively. The average seed/ripe fruit contained 57 seeds, and the mass of 1000 seeds was 2.64 g. Seed viability (100 %) was achieved in the treatment with 0.075 % tetrazolium at 45 °C for 3 h, but a germinated seed was only 13.00 %. The study of breaking seed dormancy in laboratory conditions revealed that de-operculum significantly enhanced seed germination up to 83.00 % within 15 days, compared with control treatment of 13.00 % within 34 days (p ≤ 0.01). In contrast, 10 % KNO<sub>3</sub> for 24 h under field conditions resulted in the highest seed germination rate of 91.00 % within 34 days, while de-operculum treatment showed 63.00 % of seed germination within 15 days. In addition, the seed water imbibition rate between control and de-operculum seeds was evaluated. The results demonstrated that the control seeds absorbed water more slowly than the de-operculum seeds, indicating that de-operculum promoted faster germination. The findings concluded that breaking seed dormancy is important for <em>R. tomentosa</em> seed germination. De-operculum and KNO<sub>3</sub> were discovered to be effective ways of breaking seed dormancy in <em>R. tomentosa</em>.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"42 ","pages":"Article 100558"},"PeriodicalIF":3.8000,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effective techniques to break seed dormancy in Rhodomyrtus tomentosa (Aiton) Hassk. for seed germination enhancement\",\"authors\":\"Suttiwan Wunnoo , Tewee Maneerat , Ladawan Lerslerwong , Supayang Piyawan Voravuthikunchai , Pattamavadee Kunwanlee\",\"doi\":\"10.1016/j.jarmap.2024.100558\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Rhodomyrtus tomentosa</em> is a valuable plant for medical and pharmaceutical uses. The plant reproduces through seeds; however, the seeds become dormant, resulting in low germination rates. The industrial demand for this plant is expanding, making sustainable propagation a major challenge. The present study aims to evaluate different techniques for breaking the dormancy of <em>R. tomentosa</em> seeds. A randomized design was used to evaluate different techniques for breaking the dormancy of <em>R. tomentosa</em> seeds, including de-operculum, chemical scarifications, and exogenous gibberellic acid (GA<sub>3</sub>), in both laboratory conditions at ambient temperature (25 ± 2 °C) and field conditions. The characteristics of <em>R. tomentosa</em> fruit and seeds were assessed. The average mass, width, and length of fruits were 1.90 g, 13.78 g, and 15.27 g, respectively. The average seed/ripe fruit contained 57 seeds, and the mass of 1000 seeds was 2.64 g. Seed viability (100 %) was achieved in the treatment with 0.075 % tetrazolium at 45 °C for 3 h, but a germinated seed was only 13.00 %. The study of breaking seed dormancy in laboratory conditions revealed that de-operculum significantly enhanced seed germination up to 83.00 % within 15 days, compared with control treatment of 13.00 % within 34 days (p ≤ 0.01). In contrast, 10 % KNO<sub>3</sub> for 24 h under field conditions resulted in the highest seed germination rate of 91.00 % within 34 days, while de-operculum treatment showed 63.00 % of seed germination within 15 days. In addition, the seed water imbibition rate between control and de-operculum seeds was evaluated. The results demonstrated that the control seeds absorbed water more slowly than the de-operculum seeds, indicating that de-operculum promoted faster germination. The findings concluded that breaking seed dormancy is important for <em>R. tomentosa</em> seed germination. De-operculum and KNO<sub>3</sub> were discovered to be effective ways of breaking seed dormancy in <em>R. tomentosa</em>.</p></div>\",\"PeriodicalId\":15136,\"journal\":{\"name\":\"Journal of Applied Research on Medicinal and Aromatic Plants\",\"volume\":\"42 \",\"pages\":\"Article 100558\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-05-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Research on Medicinal and Aromatic Plants\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214786124000317\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Research on Medicinal and Aromatic Plants","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214786124000317","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Effective techniques to break seed dormancy in Rhodomyrtus tomentosa (Aiton) Hassk. for seed germination enhancement
Rhodomyrtus tomentosa is a valuable plant for medical and pharmaceutical uses. The plant reproduces through seeds; however, the seeds become dormant, resulting in low germination rates. The industrial demand for this plant is expanding, making sustainable propagation a major challenge. The present study aims to evaluate different techniques for breaking the dormancy of R. tomentosa seeds. A randomized design was used to evaluate different techniques for breaking the dormancy of R. tomentosa seeds, including de-operculum, chemical scarifications, and exogenous gibberellic acid (GA3), in both laboratory conditions at ambient temperature (25 ± 2 °C) and field conditions. The characteristics of R. tomentosa fruit and seeds were assessed. The average mass, width, and length of fruits were 1.90 g, 13.78 g, and 15.27 g, respectively. The average seed/ripe fruit contained 57 seeds, and the mass of 1000 seeds was 2.64 g. Seed viability (100 %) was achieved in the treatment with 0.075 % tetrazolium at 45 °C for 3 h, but a germinated seed was only 13.00 %. The study of breaking seed dormancy in laboratory conditions revealed that de-operculum significantly enhanced seed germination up to 83.00 % within 15 days, compared with control treatment of 13.00 % within 34 days (p ≤ 0.01). In contrast, 10 % KNO3 for 24 h under field conditions resulted in the highest seed germination rate of 91.00 % within 34 days, while de-operculum treatment showed 63.00 % of seed germination within 15 days. In addition, the seed water imbibition rate between control and de-operculum seeds was evaluated. The results demonstrated that the control seeds absorbed water more slowly than the de-operculum seeds, indicating that de-operculum promoted faster germination. The findings concluded that breaking seed dormancy is important for R. tomentosa seed germination. De-operculum and KNO3 were discovered to be effective ways of breaking seed dormancy in R. tomentosa.
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JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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