Kaiyuan Li , Ninghan Xue , Songlin Jiang , Muhammad Azher Nawaz , Wenli Ji
{"title":"转录组和广泛的代谢组分析显示,激素和糖信号通路有助于新落羽杉幼树种子的正常生长","authors":"Kaiyuan Li , Ninghan Xue , Songlin Jiang , Muhammad Azher Nawaz , Wenli Ji","doi":"10.1016/j.cpb.2024.100361","DOIUrl":null,"url":null,"abstract":"<div><p>Abnormal seed growth is a problem in <em>Picea neoveitchii</em> Mast. in China that threatens the existence of this evergreen coniferous tree. However, the degree of abnormal seed growth varies in different age groups; regrettably, the causes behind abnormal seed growth at different ages are totally unclear. Thus, we compared the seeds of two ages: Gansu (GS) province, a 50-year-old tree (GS50), and a 300-year-old tree (GS300). Results indicated that 22187 unigenes were commonly found in both groups, whereas 5328 and 6079 unigenes were uniquely found in GS50 and GS300, respectively. Furthermore, a total of 5129 differentially expressed unigenes were identified between GS50 and GS300, with 2431 upregulated and 2698 downregulated. On the basis of Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, plant hormone signal transduction and starch and sucrose metabolism pathways were further selected for their potential involvement in seed growth at both ages. A wide-targeted metabolomics-based approach using liquid chromatography mass spectrometry (LC-MS) was applied to study the difference between GS50 and GS300. The results showed that there were 35 different metabolites in total being detected, mainly amino acids and sugars. Subsequently, GS50 revealed the highest number of normal seeds and the lowest number of abnormal seeds in comparison with GS300 by improving endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid 3 (GA<sub>3</sub>) contents and reducing methyl jasmonate (JA-me), abscisic acid (ABA), and brassinosteroid (BR) contents. Our research provides important evidence on the growth of seeds in different age groups of trees that might help improve seed growth in old trees.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000434/pdfft?md5=3268208c543cf301a65ed67aa3933b28&pid=1-s2.0-S2214662824000434-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Transcriptome and widely metabolomic analysis reveal hormones and sugar signaling pathways contribute to the normal growth of seeds in young Picea neoveitchii Mast. trees\",\"authors\":\"Kaiyuan Li , Ninghan Xue , Songlin Jiang , Muhammad Azher Nawaz , Wenli Ji\",\"doi\":\"10.1016/j.cpb.2024.100361\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Abnormal seed growth is a problem in <em>Picea neoveitchii</em> Mast. in China that threatens the existence of this evergreen coniferous tree. However, the degree of abnormal seed growth varies in different age groups; regrettably, the causes behind abnormal seed growth at different ages are totally unclear. Thus, we compared the seeds of two ages: Gansu (GS) province, a 50-year-old tree (GS50), and a 300-year-old tree (GS300). Results indicated that 22187 unigenes were commonly found in both groups, whereas 5328 and 6079 unigenes were uniquely found in GS50 and GS300, respectively. Furthermore, a total of 5129 differentially expressed unigenes were identified between GS50 and GS300, with 2431 upregulated and 2698 downregulated. On the basis of Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, plant hormone signal transduction and starch and sucrose metabolism pathways were further selected for their potential involvement in seed growth at both ages. A wide-targeted metabolomics-based approach using liquid chromatography mass spectrometry (LC-MS) was applied to study the difference between GS50 and GS300. The results showed that there were 35 different metabolites in total being detected, mainly amino acids and sugars. Subsequently, GS50 revealed the highest number of normal seeds and the lowest number of abnormal seeds in comparison with GS300 by improving endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid 3 (GA<sub>3</sub>) contents and reducing methyl jasmonate (JA-me), abscisic acid (ABA), and brassinosteroid (BR) contents. Our research provides important evidence on the growth of seeds in different age groups of trees that might help improve seed growth in old trees.</p></div>\",\"PeriodicalId\":38090,\"journal\":{\"name\":\"Current Plant Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000434/pdfft?md5=3268208c543cf301a65ed67aa3933b28&pid=1-s2.0-S2214662824000434-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Plant Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000434\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000434","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Transcriptome and widely metabolomic analysis reveal hormones and sugar signaling pathways contribute to the normal growth of seeds in young Picea neoveitchii Mast. trees
Abnormal seed growth is a problem in Picea neoveitchii Mast. in China that threatens the existence of this evergreen coniferous tree. However, the degree of abnormal seed growth varies in different age groups; regrettably, the causes behind abnormal seed growth at different ages are totally unclear. Thus, we compared the seeds of two ages: Gansu (GS) province, a 50-year-old tree (GS50), and a 300-year-old tree (GS300). Results indicated that 22187 unigenes were commonly found in both groups, whereas 5328 and 6079 unigenes were uniquely found in GS50 and GS300, respectively. Furthermore, a total of 5129 differentially expressed unigenes were identified between GS50 and GS300, with 2431 upregulated and 2698 downregulated. On the basis of Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, plant hormone signal transduction and starch and sucrose metabolism pathways were further selected for their potential involvement in seed growth at both ages. A wide-targeted metabolomics-based approach using liquid chromatography mass spectrometry (LC-MS) was applied to study the difference between GS50 and GS300. The results showed that there were 35 different metabolites in total being detected, mainly amino acids and sugars. Subsequently, GS50 revealed the highest number of normal seeds and the lowest number of abnormal seeds in comparison with GS300 by improving endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid 3 (GA3) contents and reducing methyl jasmonate (JA-me), abscisic acid (ABA), and brassinosteroid (BR) contents. Our research provides important evidence on the growth of seeds in different age groups of trees that might help improve seed growth in old trees.
期刊介绍:
Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.