转录组和广泛的代谢组分析显示,激素和糖信号通路有助于新落羽杉幼树种子的正常生长

IF 5.4 Q1 PLANT SCIENCES
Kaiyuan Li , Ninghan Xue , Songlin Jiang , Muhammad Azher Nawaz , Wenli Ji
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引用次数: 0

摘要

种子生长异常是中国常绿针叶树 Picea neoveitchii Mast.然而,不同树龄的种子异常生长程度各不相同;遗憾的是,不同树龄种子异常生长的原因尚不清楚。因此,我们对两个年龄段的种子进行了比较:甘肃省(GS)一棵 50 年树龄的树(GS50)和一棵 300 年树龄的树(GS300)的种子进行了比较。结果表明,22187 个单体基因普遍存在于两组中,而 5328 个单体基因和 6079 个单体基因分别存在于 GS50 和 GS300 中。此外,在 GS50 和 GS300 之间共发现了 5129 个差异表达的单基因,其中 2431 个上调,2698 个下调。在基因组百科全书(KEGG)富集分析的基础上,进一步筛选出植物激素信号转导和淀粉与蔗糖代谢途径,以确定它们可能参与了两个年龄段种子的生长。采用液相色谱-质谱(LC-MS)方法,基于广靶向代谢组学研究了 GS50 和 GS300 之间的差异。结果表明,共检测到 35 种不同的代谢物,主要是氨基酸和糖类。随后,与 GS300 相比,GS50 通过提高内源吲哚-3-乙酸(IAA)、玉米素核苷(ZR)和赤霉素 3(GA3)含量,降低茉莉酸甲酯(JA-me)、脱落酸(ABA)和类黄铜素(BR)含量,显示正常种子数量最多,异常种子数量最少。我们的研究为不同树龄组的种子生长提供了重要证据,可能有助于改善老树的种子生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome and widely metabolomic analysis reveal hormones and sugar signaling pathways contribute to the normal growth of seeds in young Picea neoveitchii Mast. trees

Abnormal seed growth is a problem in Picea neoveitchii Mast. in China that threatens the existence of this evergreen coniferous tree. However, the degree of abnormal seed growth varies in different age groups; regrettably, the causes behind abnormal seed growth at different ages are totally unclear. Thus, we compared the seeds of two ages: Gansu (GS) province, a 50-year-old tree (GS50), and a 300-year-old tree (GS300). Results indicated that 22187 unigenes were commonly found in both groups, whereas 5328 and 6079 unigenes were uniquely found in GS50 and GS300, respectively. Furthermore, a total of 5129 differentially expressed unigenes were identified between GS50 and GS300, with 2431 upregulated and 2698 downregulated. On the basis of Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, plant hormone signal transduction and starch and sucrose metabolism pathways were further selected for their potential involvement in seed growth at both ages. A wide-targeted metabolomics-based approach using liquid chromatography mass spectrometry (LC-MS) was applied to study the difference between GS50 and GS300. The results showed that there were 35 different metabolites in total being detected, mainly amino acids and sugars. Subsequently, GS50 revealed the highest number of normal seeds and the lowest number of abnormal seeds in comparison with GS300 by improving endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid 3 (GA3) contents and reducing methyl jasmonate (JA-me), abscisic acid (ABA), and brassinosteroid (BR) contents. Our research provides important evidence on the growth of seeds in different age groups of trees that might help improve seed growth in old trees.

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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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