质膜丰度决定髓系细胞的吞噬能力和功能性交叉对话。

IF 17.6 1区 医学 Q1 IMMUNOLOGY
Benjamin Y. Winer, Alexander H. Settle, Alexandrina M. Yakimov, Carlos Jeronimo, Tomi Lazarov, Murray Tipping, Michelle Saoi, Anjelique Sawh, Anna-Liisa L. Sepp, Michael Galiano, Justin S. A. Perry, Yung Yu Wong, Frederic Geissmann, Justin Cross, Ting Zhou, Lance C. Kam, H. Amalia Pasolli, Tobias Hohl, Jason G. Cyster, Orion D. Weiner, Morgan Huse
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引用次数: 0

摘要

嗜中性粒细胞和巨噬细胞等专业吞噬细胞严格控制着它们的吞噬对象、吞噬量以及货物摄取后的移动时间。我们的研究表明,质膜丰度是这些细胞行为的关键仲裁者。缺乏 G 蛋白亚基 Gβ4 的中性粒细胞和巨噬细胞表现出极度的质膜扩张,同时质膜张力明显下降。这些生物物理变化促进了对细菌、真菌、凋亡尸体和癌细胞的吞噬。我们还发现,Gβ4缺陷的中性粒细胞在摄取货物后不能正常抑制迁移。在缺乏 Gβ4 的细胞中,鞘脂合成通过驱动质膜积聚在这些表型中发挥了核心作用。在 Gβ4 基因敲除的小鼠中,中性粒细胞不仅对肺部吸入的真菌分生孢子的吞噬能力增强,而且吞噬的病原体向其他器官的迁移能力也增强了。这些结果共同揭示了一种意想不到的生物物理控制机制,它是骨髓功能决策的核心。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Plasma membrane abundance dictates phagocytic capacity and functional cross-talk in myeloid cells
Professional phagocytes like neutrophils and macrophages tightly control what they consume, how much they consume, and when they move after cargo uptake. We show that plasma membrane abundance is a key arbiter of these cellular behaviors. Neutrophils and macrophages lacking the G protein subunit Gβ4 exhibited profound plasma membrane expansion, accompanied by marked reduction in plasma membrane tension. These biophysical changes promoted the phagocytosis of bacteria, fungus, apoptotic corpses, and cancer cells. We also found that Gβ4-deficient neutrophils are defective in the normal inhibition of migration following cargo uptake. Sphingolipid synthesis played a central role in these phenotypes by driving plasma membrane accumulation in cells lacking Gβ4. In Gβ4 knockout mice, neutrophils not only exhibited enhanced phagocytosis of inhaled fungal conidia in the lung but also increased trafficking of engulfed pathogens to other organs. Together, these results reveal an unexpected, biophysical control mechanism central to myeloid functional decision-making.
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来源期刊
Science Immunology
Science Immunology Immunology and Microbiology-Immunology
CiteScore
32.90
自引率
2.00%
发文量
183
期刊介绍: Science Immunology is a peer-reviewed journal that publishes original research articles in the field of immunology. The journal encourages the submission of research findings from all areas of immunology, including studies on innate and adaptive immunity, immune cell development and differentiation, immunogenomics, systems immunology, structural immunology, antigen presentation, immunometabolism, and mucosal immunology. Additionally, the journal covers research on immune contributions to health and disease, such as host defense, inflammation, cancer immunology, autoimmunity, allergy, transplantation, and immunodeficiency. Science Immunology maintains the same high-quality standard as other journals in the Science family and aims to facilitate understanding of the immune system by showcasing innovative advances in immunology research from all organisms and model systems, including humans.
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