通过定制的丝绸反向蛋白石支架调节细菌培养。

IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Bei Qi, Yitan Li, Junyan Zhao, Jiapeng Zhang, Xiaohua Zhang, Gaojian Chen, Zhaohui Yang
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引用次数: 0

摘要

控制微生物群的生长在生物医学领域具有重要意义。通过制造孔径从 0.3 μm 到 4.5 μm 不等的丝反蛋白石(SIO)支架,实现了细菌的选择性生长。孔径大小对单一菌种和混合菌种培养物中细菌的生长动态有很大影响。据观察,SIO-4.5 μm支架更有利于培养金黄色葡萄球菌,而SIO-0.3 μm支架则更适合培养大肠杆菌和绿脓杆菌。通过调整蚕丝纤维蛋白的次级构象,SIO 基质的硬度将发生改变,这使得 SIO 上的细菌数量比蚕丝纤维蛋白薄膜(SFF)上的细菌数量增加了 16 倍。通过调节孔径可以将金黄色葡萄球菌与铜绿假单胞菌的比例从0.8调整到9.3,这凸显了这种方法在调节细菌培养方面的潜力。本文受版权保护。保留所有权利。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Regulating Bacterial Culture through Tailored Silk Inverse Opal Scaffolds

Regulating Bacterial Culture through Tailored Silk Inverse Opal Scaffolds

Controlling the growth of microbial consortia is of great significance in the biomedical field. Selective bacterial growth is achieved by fabricating silk inverse opal (SIO) scaffolds with varying pore sizes ranging from 0.3 to 4.5 µm. Pore size significantly influences the growth dynamics of bacteria in both single and mixed-strain cultures. Specially, the SIO-4.5 µm scaffold is observed to be more favorable for cultivating S. aureus, whereas the SIO-0.3 µm scaffold is more suitable for cultivating E. coli and P. aeruginosa. By adjusting the secondary conformation of silk fibroin, the stiffness of the SIO substrate will be altered, which results in the increase of bacteria on the SIO by 16 times compared with that on the silk fibroin film. Manipulating the pore size allows for the adjustment of the S. aureus to P. aeruginosa ratio from 0.8 to 9.3, highlighting the potential of this approach in regulating bacterial culture.

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来源期刊
Macromolecular bioscience
Macromolecular bioscience 生物-材料科学:生物材料
CiteScore
7.90
自引率
2.20%
发文量
211
审稿时长
1.5 months
期刊介绍: Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.
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