全面分析酵母 atg 突变体的非选择性和选择性自噬,以及 Atg8 连接系统缺失时自噬活性的特征。

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tamara Ginevskaia, Aleksei Innokentev, Kentaro Furukawa, Tomoyuki Fukuda, Manabu Hayatsu, Shun-Ichi Yamashita, Keiichi Inoue, Shinsuke Shibata, Tomotake Kanki
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引用次数: 0

摘要

大多数自噬相关基因或 ATG 基因都是在利用芽殖酵母进行的研究中发现的。虽然 ATG 基因的功能已广为人知,但各个基因对非选择性自噬和各种选择性自噬的贡献仍有待全面阐明。在这项研究中,我们量化了所有酿酒酵母 atg 突变体中的非选择性自噬、细胞质到液泡靶向(Cvt)途径、有丝分裂、内质网(ER)自噬和pexophagy 的活性。在被认为对自噬至关重要的核心自噬基因突变体中,atg13 突变体和参与两个泛素样连接系统的基因突变体保留了残余的自噬功能。特别是 Atg8 泛素样连接系统(Atg8 系统)的突变体表现出大量的非选择性自噬、Cvt 途径和pexophagy,但丝裂噬和ER-噬却检测不到。Atg8 系统突变体也显示出类似自噬体的泡内囊泡,尽管其大小和频率明显降低。因此,我们的数据表明,在没有 Atg8 系统的情况下,自噬货物的膜螯合和液泡输送也会发生。除了这些发现,本文进行的综合分析还为未来的自噬研究提供了宝贵的数据集。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comprehensive analysis of non-selective and selective autophagy in yeast atg mutants and characterization of autophagic activity in the absence of the Atg8 conjugation system.

Most autophagy-related genes, or ATG genes, have been identified through studies using budding yeast. Although the functions of the ATG genes are well understood, the contributions of individual genes to non-selective and various types of selective autophagy remain to be fully elucidated. In this study, we quantified the activity of non-selective autophagy, the cytoplasm-to-vacuole targeting (Cvt) pathway, mitophagy, endoplasmic reticulum (ER)-phagy and pexophagy in all Saccharomyces cerevisiae atg mutants. Among the mutants of the core autophagy genes considered essential for autophagy, the atg13 mutant and mutants of the genes involved in the two ubiquitin-like conjugation systems retained residual autophagic functionality. In particular, mutants of the Atg8 ubiquitin-like conjugation system (the Atg8 system) exhibited substantial levels of non-selective autophagy, the Cvt pathway and pexophagy, although mitophagy and ER-phagy were undetectable. Atg8-system mutants also displayed intravacuolar vesicles resembling autophagic bodies, albeit at significantly reduced size and frequency. Thus, our data suggest that membranous sequestration and vacuolar delivery of autophagic cargo can occur in the absence of the Atg8 system. Alongside these findings, the comprehensive analysis conducted here provides valuable datasets for future autophagy research.

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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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