枯草芽孢杆菌的 CssRS 双组分系统有助于茶青霉素和多粘菌素 B 的反应。

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Alexandr V Kachan, Anatoly N Evtushenkov
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引用次数: 0

摘要

CssRS 是一个双组分系统,在介导枯草芽孢杆菌的分泌应激反应中起着关键作用。该系统能上调膜结合型 HtrA 家族蛋白酶的合成,这些蛋白酶能处理因过度表达或热休克而在细胞包膜内积累的折叠错误的蛋白质。最近的研究表明,细胞包膜应激反应会诱导 CssRS 调控的基因。我们研究了 CssRS 调控的 htrA 启动子在不同细胞壁和膜活性物质存在下的诱导情况,观察到糖肽(万古霉素和替考拉宁)、多粘菌素 B 和 E、某些 β-内酰胺和去污剂诱导了 CssRS 控制的基因。Teicoplanin 和多粘菌素 B 以依赖 CssRS 的方式诱导 spxO 基因的表达,导致 Spx(枯草芽孢杆菌中二硫化物应激的主调节因子)的活性增加。研究证明,CssRS 信号通路和 Spx 活性参与了枯草芽孢杆菌对 teicoplanin 和多粘菌素 B 的抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The CssRS two-component system of Bacillus subtilis contributes to teicoplanin and polymyxin B response.

The CssRS two-component system of Bacillus subtilis contributes to teicoplanin and polymyxin B response.

CssRS is a two-component system that plays a pivotal role in mediating the secretion stress response in Bacillus subtilis. This system upregulates the synthesis of membrane-bound HtrA family proteases that cope with misfolded proteins that accumulate within the cell envelope as a result of overexpression or heat shock. Recent studies have shown the induction of CssRS-regulated genes in response to cell envelope stress. We investigated the induction of the CssRS-regulated htrA promoter in the presence of different cell wall- and membrane-active substances and observed induction of the CssRS-controlled genes by glycopeptides (vancomycin and teicoplanin), polymyxins B and E, certain β-lactams, and detergents. Teicoplanin was shown to elicit remarkably stronger induction than vancomycin and polymyxin B. Teicoplanin and polymyxin B induced the spxO gene expression in a CssRS-dependent fashion, resulting in increased activity of Spx, a master regulator of disulfide stress in Bacillus subtilis. The CssRS signaling pathway and Spx activity were demonstrated to be involved in Bacillus subtilis resistance to teicoplanin and polymyxin B.

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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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