肺癌治疗的革命:创新的 CRISPR-Cas9 传输策略。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Dilpreet Singh
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引用次数: 0

摘要

肺癌,包括非小细胞肺癌(NSCLC)和小细胞肺癌(SCLC),因其发病率和死亡率高,仍然是全球健康面临的重大挑战。本综述旨在仔细研究目前在利用 CRISPR-Cas9 基因编辑技术治疗肺癌方面的进展和策略。这项技术预示着分子生物学进入了一个新时代,可提供前所未有的精确基因组修饰。然而,其治疗潜力取决于有效递送机制的开发,以确保基因编辑工具高效、特异性地输送到肿瘤细胞。我们探讨了病毒载体、脂基纳米颗粒和物理方法等多种递送方法,重点介绍了它们各自的优势、局限性和最新突破。本综述还深入探讨了这些策略的转化和临床意义,讨论了研究 CRISPR-Cas9 移植治疗肺癌的可行性、有效性和安全性的临床前和临床研究。通过仔细研究正在进行的临床试验并提供转化观点,我们旨在阐明这一快速发展领域的现状和未来方向。综述的结构首先介绍了肺癌的问题和意义,然后概述了 CRISPR-Cas9 技术,详细研究了递送策略,并分析了临床应用和监管方面的注意事项。最后,我们讨论了未来的前景和挑战,如优化递送策略、提高特异性、减轻免疫原性问题以及解决监管问题。本综述旨在深入探讨 CRISPR-Cas9 作为肺癌靶向治疗和个性化医疗的革命性方法的潜力,并强调递送策略的开发对于充分发挥这一突破性技术潜力的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Revolutionizing Lung Cancer Treatment: Innovative CRISPR-Cas9 Delivery Strategies

Revolutionizing Lung Cancer Treatment: Innovative CRISPR-Cas9 Delivery Strategies

Lung carcinoma, including both non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC), remains a significant global health challenge due to its high morbidity and mortality rates. The objsective of this review is to meticulously examine the current advancements and strategies in the delivery of CRISPR-Cas9 gene-editing technology for the treatment of lung carcinoma. This technology heralds a new era in molecular biology, offering unprecedented precision in genomic modifications. However, its therapeutic potential is contingent upon the development of effective delivery mechanisms that ensure the efficient and specific transport of gene-editing tools to tumor cells. We explore a variety of delivery approaches, such as viral vectors, lipid-based nanoparticles, and physical methods, highlighting their respective advantages, limitations, and recent breakthroughs. This review also delves into the translational and clinical significance of these strategies, discussing preclinical and clinical studies that investigate the feasibility, efficacy, and safety of CRISPR-Cas9 delivery for lung carcinoma. By scrutinizing the landscape of ongoing clinical trials and offering translational perspectives, we aim to elucidate the current state and future directions of this rapidly evolving field. The review is structured to first introduce the problem and significance of lung carcinoma, followed by an overview of CRISPR-Cas9 technology, a detailed examination of delivery strategies, and an analysis of clinical applications and regulatory considerations. Our discussion concludes with future perspectives and challenges, such as optimizing delivery strategies, enhancing specificity, mitigating immunogenicity concerns, and addressing regulatory issues. This comprehensive overview seeks to provide insights into the potential of CRISPR-Cas9 as a revolutionary approach for targeted therapies and personalized medicine in lung carcinoma, emphasizing the importance of delivery strategy development in realizing the full potential of this groundbreaking technology.

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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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