乌贼皂苷提取物的提取、鉴定及抗真菌特性评估

Mohammad Alkhuzaie, H. Kredy, Sajid. H. Guzar, Bassem Charfeddine, Ouahchi Ines
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引用次数: 0

摘要

高效液相色谱法(HPLC)分析表明,皂苷提取物中含有一种名为 Hederagenin 苷的重要成分。此外,还利用紫外可见光谱测定了皂苷提取物活性基团的波长和电子跃迁。使用 Phenomenex 公司生产的 Gemini C18(5 µm)固定相取得了最佳结果。他们通过评估反相 C18 色谱柱上的几种流动相,确定了增强 LC/MS 分析的最佳色谱条件。 说明了在物质质谱中观察到的预期碎片模式。测定了对黄曲霉、尼日尔曲霉和交替曲霉的抗真菌活性。与对照组相比,三萜皂苷提取物在浓度(100 ppm)下对黄曲霉和尼日尔曲霉(2 厘米)等病原真菌的生长活性有最大的抑制作用,在浓度(25 ppm)下对黄曲霉(5 厘米)的生长活性抑制作用最小。三萜皂苷萃取物对交替曲霉、尼日尔曲霉等真菌的生长活性具有抑制作用。另一方面,三萜皂苷提取物浓度越高,人体血液中溶血活性的百分比也越高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extraction and Identification of saponin extracts from Lepidium aucheri Boiss and antifungal properties evaluation
The analysis by High-performance liquid chromatography (HPLC) revealed that the saponin extracts contain a significant component called (Hederagenin glycoside). The wavelengths and electronic transitions of the active groups of the saponin extract were also determined using the UV-Vis Spectra. The optimal result was obtained by utilizing the Gemini C18 (5 µm) stationary phase manufactured by Phenomenex. They determine the optimal chromatographic conditions for enhancing LC/MS analysis by evaluating several mobile phases on a reversed-phase C18 column.  Illustrates the anticipated patterns of fragmentation observed in the mass spectra of substances. The Antifungal activity against Aspergillus flavus, Aspergillus Niger, and Aspergillus Alternaria was determined. The inhibitory effect in vitro was defined to appear, triterpenoid saponins extract showed maximum growth activity inhibitory of fungi against pathogens like Aspergillus Alternaria and Aspergillus Niger (2 cm), at the concentration (100 ppm) and the minimum growth activity inhibitory was against Aspergillus Alternaria (5 cm) at a concentration (25 ppm) compare with control. The triterpenoid saponins extract its ability to grow activity inhibition of fungi type Aspergillus Alternaria, Aspergillus Niger. On the other hand, the percentage of hemolysis activity in human blood increases with a high concentration of triterpenoid Saponins extract.
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