不同水平的精料对肉用小母牛循环细胞因子浓度的影响

IF 1.3 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Translational Animal Science Pub Date : 2024-06-02 DOI:10.1093/tas/txae089

Erin L Stockland, Molly S Smith, A. Pickett, R. F. Cooke, R. Poole

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引用次数: 0

摘要

免疫系统的成分（如细胞因子和趋化因子）可影响繁殖效率。确定营养对循环细胞因子浓度变化的影响将有助于更好地了解肉牛的繁殖效率。本研究旨在确定日粮组成对肉用小母牛循环细胞因子浓度的影响。采用 3 x 3 拉丁正方形设计，给青春期受金牛瘤胃影响的小母牛（n=15）饲喂不同精料百分比的日粮。处理期包括 28 天的饲喂期和 21 天的冲洗间隔期。处理组分别饲喂100%的干草（高饲草；HF）、60%的干草和40%的玉米精料（中等；INT）以及25%的干草和75%的玉米精料（高谷物；HG）。母牛每天的饲料量为体重的 2%。在治疗期的第 0 天和第 28 天采集血液进行细胞因子分析。根据生产商的说明，使用 RayBiotech Quantibody Bovine Cytokine Array Q1 试剂盒对血浆细胞因子浓度进行量化。干扰素γ诱导蛋白10（IP10）的浓度随着精料日粮的增加呈线性下降（P = 0.037）。摄入 HF 日粮的小母牛与摄入 HG 日粮的小母牛的 IP10 浓度不同（分别为 3069.52 vs. 1001.84 ± 669.01 pg/mL），摄入 INT 日粮的小母牛与摄入 HG 日粮的小母牛的 IP10 浓度也不同（分别为 2886.77 vs. 1001.84 ± 669.01 pg/mL）；但是，HF 和 INT 小母牛的 IP10 浓度没有显著差异。与 INT 日粮相比，食用 HG 日粮的母牛的白细胞介素-1 家族成员 5 (IL1F5) 浓度有降低的趋势（P = 0.08）。结果表明，摄入高浓缩日粮的小母牛体内 IP10 和 IL1F5 的浓度较低。要更好地了解日粮对发育中小牛免疫系统的影响，还需要进行更多的研究。

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Influence of differing levels of concentrate on circulating cytokine concentrations in beef heifers

Components of the immune system (e.g., cytokines and chemokines) can influence reproductive efficiency. Characterizing the influence nutrition has on shifts in circulating cytokine concentrations will allow for a better understanding of reproductive efficiency in beef cattle. This study aimed to determine the effect of diet composition on circulating cytokine concentrations of beef heifers. Using a 3 x 3 Latin square design, pubertal Bos taurus-influenced rumen-cannulated heifers (n=15) were fed a diet based on different concentrate percentages. The treatment period consisted of 28-day feeding periods with a washout interval of 21 days. Treatment groups were fed 100% grass hay (high forage; HF), 60% grass hay with 40% corn-based concentrate (intermediate; INT), and 25% grass hay with 75% corn-based concentrate (high grain; HG). Heifers were offered 2% of their body weight in feed daily. Blood was collected on day 0 and 28 of treatment period for cytokine analysis. Plasma cytokine concentrations were quantified using RayBiotech Quantibody Bovine Cytokine Array Q1 kit according to manufacturer instructions. Concentrations of interferon gamma-induced protein 10 (IP10) linearly decreased with an increased concentrate diet (P = 0.037). Concentrations of IP10 differed for heifers consuming HF diet versus HG diet (3069.52 vs. 1001.84 ± 669.01 pg/mL, respectively) and heifers consuming INT diet versus HG diet (2886.77 vs. 1001.84 ± 669.01 pg/mL, respectively); however, there were no significant differences in IP10 concentrations between HF and INT heifers. There was a tendency for interleukin-1 family member 5 (IL1F5) concentrations to be lower for heifers consuming the HG diet compared to INT diet (P = 0.08). Results suggest that heifers consuming a high-concentrate diet have lower concentrations of IP10 and IL1F5. Additional research is necessary to better understand the dietary influence on the immune system in developing heifers.

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来源期刊

Translational Animal Science Veterinary-Veterinary (all)

CiteScore

2.80

自引率

15.40%

发文量

149

审稿时长

8 weeks

期刊介绍： Translational Animal Science (TAS) is the first open access-open review animal science journal, encompassing a broad scope of research topics in animal science. TAS focuses on translating basic science to innovation, and validation of these innovations by various segments of the allied animal industry. Readers of TAS will typically represent education, industry, and government, including research, teaching, administration, extension, management, quality assurance, product development, and technical services. Those interested in TAS typically include animal breeders, economists, embryologists, engineers, food scientists, geneticists, microbiologists, nutritionists, veterinarians, physiologists, processors, public health professionals, and others with an interest in animal production and applied aspects of animal sciences.