开发同时针对甲型流感病毒和 SARS-CoV-2 的具有抗病毒特性的面霜

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Philippe Mangeot, K. Lazou, Aurelia Blin, Valerie Gorzelanczyk, Olivier Jeanneton, Chantal Kurfurst, Karl Pays, Bruno Bavouzet, C. Nizard, T. Ohlmann, A. Bulteau
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引用次数: 0

摘要

目的:许多严重急性呼吸道感染都是由病毒病原体引起的,病毒是造成全球大量死亡的原因。最常见的呼吸道病毒包括甲型流感病毒(IAV)和最近于 2019 年出现的 SARS-CoV-2 病毒,后者引发了 21 世纪初最重要的人类大流行。IAV 和 SARS-CoV-2 都有共同的临床特征,并通过气溶胶和飞沫传播病毒颗粒。这些病毒颗粒从口鼻进入宿主体内,或通过不同媒介的接触污染进行间接传播。这些事实促使我们研究设计一种具有针对 IAV 和 SARS-CoV-2 杀病毒活性的软膏的可能性。研究方法我们首先研究了已知具有抗病毒特性的化合物(如环糊精或含有硫酸化多糖的藻类提取物)对用 IAV 的蛋白 HA(血凝素)和 NA(神经氨酸酶)或水泡性口炎病毒的 G 蛋白或带穗颗粒伪型慢病毒病毒颗粒(VP)感染的培养细胞的作用,以便在人类胚胎肾脏(HEK293T)细胞中筛选出具有抗病毒活性的分子。结果我们的研究结果表明,在皮肤上接种病毒之前涂抹一些含环糊精的药膏,可显著降低 HANA 和穗状病毒颗粒的稳定性。结论:我们观察到这些药膏对 IAV 或 SARS-CoV-2 有一定的特异性,这表明病毒活性的中和与这两种病原体的受体相互作用和进入机制有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Towards the Development of a Cream with Antiviral Properties Targeting Both the Influenza A Virus and SARS-CoV-2
Objective: Many severe acute respiratory infections are caused by viral pathogens, and viruses are responsible for a large number of deaths worldwide. Among the most common respiratory viruses are the influenza A virus (IAV) and, more recently, the SARS-CoV-2 that emerged in 2019 and caused the most significant human pandemic of the beginning of the 21st century. Both IAV and SARS-CoV-2 share clinical features and a common transmission route through the emission of viral particles via aerosols and droplets. These penetrate the host after entry from the nose and mouth or an indirect mode of transmission via contact contamination of different media. These facts prompted us to investigate the possibility of designing a soft cream with a virucidal activity targeted against IAV and SARS-CoV-2. Methods: We first investigated the action of chemical compounds known to have antiviral properties such as cyclodextrin, or algae extracts containing sulfated polysaccharides, on cultured cells infected with lentiviral viral particles pseudotyped (VP) with either proteins HA (hemagglutinin) and NA (neuraminidase) from IAV or the G protein from the vesicular stomatitis virus or spike-bearing particles in order to select molecules with antiviral activities in human embryonic kidney (HEK293T) cells. Results: Our results show that some cyclodextrin-containing creams can significantly reduce the stability of HANA- and spike-bearing particles when they are applied prior to challenge with a viral inoculum on skin. Conclusions: We observed some specificities of these creams towards either IAV or SARS-CoV-2, indicating that the neutralization of viral activity is correlated with the mechanism of receptor interaction and entry of these two pathogens.
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来源期刊
Cosmetics
Cosmetics Medicine-Surgery
CiteScore
5.20
自引率
12.10%
发文量
108
审稿时长
8 weeks
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