人类 80S 核糖体亚 2 Å 分辨率结构中的化学修饰、离子和水分子

IF 12.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature Structural & Molecular Biology Pub Date : 2024-06-06 DOI:10.1038/s41594-024-01275-w

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引用次数: 0

摘要

我们利用新一代低温电子显微镜和质谱技术，在全人类 80S 核糖体的亚 2 Å 分辨率结构中发现了 235 种化学修饰。新发现的 rRNA 修饰为核糖和尿苷酸创造了新的氢键模式。离子可视化显示，与 Mg2+ 相关的水分子不同程度地被侧链取代。这项研究为稳定 A-U 或 A-Ψ 碱基对和 RNA 蛋白相互作用提供了分子基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Chemical modifications, ions and water molecules in the sub-2 Å resolution structure of the human 80S ribosome

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Chemical modifications, ions and water molecules in the sub-2 Å resolution structure of the human 80S ribosome

Using next-generation cryo-EM and mass spectrometry, we identified 235 chemical modifications in the sub-2 Å resolution structure of the full human 80S ribosome. The newly identified rRNA modifications were found to create new hydrogen bond patterns for riboses and uridines. Ion visualization revealed that Mg2+-associated water molecules are variably substituted by side chains. This study provides the molecular basis for the stabilization of A–U or A–Ψ base pairs and RNA–protein interactions.

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来源期刊

Nature Structural & Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOPHYSICS

CiteScore

22.00

自引率

1.80%

发文量

160

审稿时长

3-8 weeks

期刊介绍： Nature Structural & Molecular Biology is a comprehensive platform that combines structural and molecular research. Our journal focuses on exploring the functional and mechanistic aspects of biological processes, emphasizing how molecular components collaborate to achieve a particular function. While structural data can shed light on these insights, our publication does not require them as a prerequisite.