Wei Deng, Yue Zhou, Qinlin Wan, Lei Li, Hui Deng, Yong Yin, Qingsong Zhou, Qiujiang Li, Duo Cheng, Xuefeng Hu, Yunbing Wang and Ganjun Feng
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引用次数: 0
摘要
设计人工纳米酶清除软骨细胞(CHOs)中的活性氧(ROS)被认为是治疗骨关节炎(OA)最可行的途径。然而,由于纳米酶催化位点的暴露量和供氧不足导致的 ROS 积累严重威胁着这种疗法的临床应用。尽管人们已经广泛研究了金属有机框架(MOF)固定人工纳米酶以提高活性位点的暴露量,但在治疗 OA 时用于清除 ROS 的人工纳米酶/MOFs 仍然缺乏。本研究设计了一种生物相容性润滑水凝胶负载铁掺杂沸石咪唑酸框架-8(Fe/ZIF-8/Gel)中心酶,作为一种三元人工纳米酶,它能清除内源性过量表达的 ROS,协同产生溶解氧并增强 CHOs 的持续润滑性。这种特性使纳米酶水凝胶能够成功缓解 OA 缺氧和抑制氧化应激损伤。基于三元策略的疗法在体内显示出良好的软骨修复效果。实验结果表明,纳米酶增强型润滑水凝胶是一种潜在的有效治疗 OA 的新策略。
Nano-enzyme hydrogels for cartilage repair effectiveness based on ternary strategy therapy†
Designing artificial nano-enzymes for scavenging reactive oxygen species (ROS) in chondrocytes (CHOs) is considered the most feasible pathway for the treatment of osteoarthritis (OA). However, the accumulation of ROS due to the amount of nano-enzymatic catalytic site exposure and insufficient oxygen supply seriously threatens the clinical application of this therapy. Although metal–organic framework (MOF) immobilization of artificial nano-enzymes to enhance active site exposure has been extensively studied, artificial nano-enzymes/MOFs for ROS scavenging in OA treatment are still lacking. In this study, a biocompatible lubricating hydrogel-loaded iron-doped zeolitic imidazolate framework-8 (Fe/ZIF-8/Gel) centrase was engineered to scavenge endogenous overexpressed ROS synergistically generating dissolved oxygen and enhancing sustained lubrication for CHOs as a ternary artificial nano-enzyme. This property enabled the nano-enzymatic hydrogels to mitigate OA hypoxia and inhibit oxidative stress damage successfully. Ternary strategy-based therapies show excellent cartilage repair in vivo. The experimental results suggest that nano-enzyme-enhanced lubricating hydrogels are a potentially effective OA treatment and a novel strategy.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices