将油棕种植园中的植物生长促进根瘤菌作为灵芝生物控制剂的生物勘探研究。

Q3 Agricultural and Biological Sciences
Yurnaliza Yurnaliza, Isnaini Nurwahyuni, Sovia Lenny, Anisa Lutfia
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引用次数: 0

摘要

<b>背景和目的:</b>油棕榈研究的优先事项包括探索新型细菌分离物作为抑制<i>灵芝</i>的可能制剂。本研究的目的是评估和鉴定从油棕植物根瘤菌层中获得的根瘤菌在抗<i>灵芝</i>活性方面的潜力。通过定性检测来评估某些分离菌株的抗真菌活性以及几丁质酶和葡聚糖酶的合成情况。使用 16S-rRNA 核糖体引物,特别是 27F 和 1492R 引物,对候选菌株进行了分子鉴定。 <b>结果:</b> 研究结果表明,与其他地点相比,政府种植园的重氮营养细菌和本地细菌数量比例最高。在九十个细菌分离物中,有二十一个分离物的子集通过双重培养实验证明有能力阻碍 G. boninense<i>/i> 的发展。发现 21 株细菌具有抗真菌活性。根据序列分析发现了九种可能的细菌。这些细菌包括:Burkholderia territorii</i> (RK2, RP2, RP3, RP5)、Burkholderia stagnalis</i> (RK3), <i>;(RP1), <i>Serratia marcescens</i> (RP13) 和 <i>Rhizobium multihospitium</i> (RU4)。<b>结论:</b>研究结果表明,相当一部分细菌群体具有固氮、产生吲哚-3-乙酸(IAA)和溶解磷酸盐的能力。不过,值得注意的是,RU4 没有表现出磷酸盐溶解能力,而 RK2 则没有表现出 IAA 生产能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioprospecting Study of Plant Growth Promoting Rhizospheric Bacteria from Oil Palm Plantation as Biological Control Agent of Ganoderma boninense.

<b>Background and Objective:</b> The prioritisation of oil palm studies involves the exploration of novel bacterial isolates as possible agents for suppressing <i>Ganoderma boninense</i>. The objective of this study was to evaluate and characterise the potential of rhizospheric bacteria, obtained from the rhizosphere of oil palm plants, in terms of their ability to demonstrate anti-<i>Ganoderma </i>activity. <b>Materials and Methods:</b> The study began by employing a dual culture technique to select hostile bacteria. Qualitative detection was performed to assess the antifungal activity, as well as the synthesis of chitinase and glucanase, from certain isolates. The candidate strains were molecularly identified using 16S-rRNA ribosomal primers, specifically the 27F and 1492R primers. <b>Results:</b> The findings of the study indicated that the governmental plantation exhibited the highest ratio between diazotroph and indigenous bacterial populations in comparison to the other sites. Out of a pool of ninety bacterial isolates, a subset of twenty-one isolates demonstrated the ability to impede the development of <i>G. boninense</i>, as determined using a dual culture experiment. Twenty-one bacterial strains were found to exhibit antifungal activity. Nine possible bacteria were found based on the sequence analysis. These bacteria include <i>Burkholderia territorii</i> (RK2, RP2, RP3, RP5), <i>Burkholderia stagnalis</i> (RK3), <i>Burkholderia cenocepacia</i> (RP1), <i>Serratia marcescens</i> (RP13) and <i>Rhizobium multihospitium</i> (RU4). <b>Conclusion:</b> The findings of the study revealed that a significant proportion of the bacterial population exhibited the ability to perform nitrogen fixation, indole-3-acetic acid (IAA) production and phosphate solubilization. However, it is worth noting that <i>Rhizobium multihospitium</i> RU4 did not demonstrate the capacity for phosphate solubilization, while <i>B. territory</i> RK2 did not exhibit IAA production.

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来源期刊
Pakistan Journal of Biological Sciences
Pakistan Journal of Biological Sciences Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.90
自引率
0.00%
发文量
102
期刊介绍: Pakistan Journal of Biological Sciences (PJBS) is an international, peer-reviewed and well indexed scientific journal seeks to promote and disseminate the knowledge of biological sciences by publishing outstanding research in the field. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, entomology, toxicology, molecular biology & genetics, biochemistry, biotechnology, biodiversity, ecology, marine biology, plant biology and bioinformatics.
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