用于电流体的新型 3D 纺织结构和几何形状。

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Sujani B. Y. Abeywardena, Zhilian Yue, Gordon G. Wallace, Peter C. Innis
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引用次数: 0

摘要

微流体与电场控制的集成(通常称为电流体)为生物医学分析带来了新的机遇。微流体技术中对封闭微毛细管通道的要求通常是通过复杂的微光刻制造方法形成的,这限制了传统电流体设备分离过程的直接访问性。纺织品结构提供了一种低成本的替代方法,通过提供开放的、表面可触及的毛细管通道来克服这些限制。在此,我们研究了不同三维纺织结构在电流体方面的应用潜力。在这项研究中,我们在不同直径的尼龙单丝芯上编织了 12 根聚酯纱,制成了功能性三维芯壳纺织结构。根据荧光标记化合物的迁移率和带宽,评估了这些三维核壳织物结构在去除尼龙芯之前和之后的毛细管电泳性能。结果表明,纤维排列和密度决定了这些纺织结构中固有毛细管的形成,这也影响了电泳研究中溶质分析流动性和分离带宽。以 0.47 毫米尼龙为核心的核壳织物结构表现出最高的荧光素迁移率和较窄的分离带宽。通过对中心尼龙芯进行简单的热固化,这种最佳纺织结构很容易转换成不同的几何形状。这种方法可用于制造一系列微型装置,这些装置具有电流体学所需的许多基本功能,同时还能保持开放的表面通路,而传统方法则无法做到这一点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Novel 3D textile structures and geometries for electrofluidics

Novel 3D textile structures and geometries for electrofluidics

The integration of microfluidics with electric field control, commonly referred to as electrofluidics, has led to new opportunities for biomedical analysis. The requirement for closed microcapillary channels in microfluidics, typically formed via complex microlithographic fabrication approaches, limits the direct accessibility to the separation processes during conventional electrofluidic devices. Textile structures provide an alternative and low-cost approach to overcome these limitations via providing open and surface-accessible capillary channels. Herein, we investigate the potential of different 3D textile structures for electrofluidics. In this study, 12 polyester yarns were braided around nylon monofilament cores of different diameters to produce functional 3D core–shell textile structures. Capillary electrophoresis performances of these 3D core–shell textile structures both before and after removing the nylon core were evaluated in terms of mobility and bandwidth of a fluorescence marker compound. It was shown that the fibre arrangement and density govern the inherent capillary formation within these textile structures which also impacts upon the solute analyte mobility and separation bandwidth during electrophoretic studies. Core–shell textile structures with a 0.47 mm nylon core exhibited the highest fluorescein mobility and presented a narrower separation bandwidth. This optimal textile structure was readily converted to different geometries via a simple heat-setting of the central nylon core. This approach can be used to fabricate an array of miniaturized devices that possess many of the basic functionalities required in electrofluidics while maintaining open surface access that is otherwise impractical in classical approaches.

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来源期刊
ELECTROPHORESIS
ELECTROPHORESIS 生物-分析化学
CiteScore
6.30
自引率
13.80%
发文量
244
审稿时长
1.9 months
期刊介绍: ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.
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