Bela H. Mishal, Sancharini Das, Vaishnavi N. Mahajan, Mahesh S. Dharne, Rakesh S. Joshi and Ashok P. Giri*,
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引用次数: 0
摘要
青霉素 V(苯氧甲基青霉素)因其优异的酸稳定性和对常见皮肤和呼吸道感染的有效性,在天然青霉素中备受青睐。鉴于其广泛的治疗用途,有必要建立一种更环保的方法来最大限度地回收青霉素 V,以减少碳足迹。在此,我们确定并验证了基于树脂的青霉素 V 回收的优化操作条件。结果表明,在筛选出的其他树脂中,Amberlite XAD4 对青霉素 V 的疏水吸附能力最高。使用线性和非线性回归分析进行的动力学和等温线研究表明,吸附过程非常符合伪二阶动力学(R2 = 0.9816)和 Freundlich 吸附等温线模型(R2 = 0.9871)。吸附平衡在 4 小时内达到,在青霉素 V 浓度为 3 毫克/毫升时吸附量最大。此外,还将优化后的提取方案与传统的基于醋酸丁酯的下游处理方法进行了比较。在最佳条件下,树脂法青霉素 V 的回收率比溶剂萃取法高 2 倍,而且树脂可重复使用 6 次以上而不影响产量。这些发现标志着在开发一种环境可持续的青霉素 V 回收方法方面取得了重大进展,同时也为萃取发酵提供了一种潜在的可行方法。
An Adsorption Based Downstream Processing Approach for Penicillin V from a Penicillium chrysogenum BIONCL I22 Culture Filtrate
Penicillin V (phenoxy methyl penicillin) is highly sought after among natural penicillins because of its exceptional acid stability and effectiveness against common skin and respiratory infections. Given its wide-ranging therapeutic uses, there is a need to establish a greener method for its maximum recovery to reduce the carbon footprint. Here, we have identified and validated optimized operational conditions for resin-based penicillin V recovery. It was observed that Amberlite XAD4 had the highest penicillin V hydrophobic adsorption capacity among the other screened resins. Kinetic and isothermal studies using linear and nonlinear regression analysis showed that the adsorption process well fitted with pseudo-second-order kinetics (R2 = 0.9816) and the Freundlich adsorption isotherm model (R2 = 0.9871). Adsorption equilibrium was attained within 4 h, while maximum adsorption was observed at 3 mg/mL penicillin V concentration. Furthermore, the optimized extraction protocol was compared with the conventional butyl acetate-based downstream processing. Under optimum conditions resin-based penicillin V recovery was 2-fold higher as compared to the solvent extraction method and the resin could be reused for over six cycles without compromising the yield. These findings signify substantial progress toward the development of an environmentally sustainable approach for penicillin V recovery and a potentially viable method for extractive fermentation.
ACS OmegaChemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍:
ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.