碳酸氢钠溶液对大鼠杏仁核神经元中超重力诱导的 Fos 表达的影响:碳酸氢钠治疗眩晕的意义

IF 1.6 4区 医学 Q2 OTORHINOLARYNGOLOGY
Junya Fukuda , Kazunori Matsuda , Go Sato , Yoshiaki Kitamura , Atsuhiko Uno , Noriaki Takeda
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引用次数: 0

摘要

目的 在日本,静脉注射 7% 的碳酸氢钠(NaHCO3)溶液最初是为了抑制晕车,后来一直被用于治疗眩晕症。此前,我们曾报道过大鼠在接受超重力刺激后,杏仁核中会出现 Fos 阳性神经元。在本研究中,我们考察了注射 7 % NaHCO3 是否能抑制大鼠杏仁核中央核神经元中超重力诱导的 Fos 表达。结果与只暴露于离心机噪音和接受 7% NaHCO3 溶液的大鼠相比,未接受药物或生理盐水的大鼠在 2 G 超重力后杏仁核中央核中 Fos 阳性神经元的数量显著增加。结论由于 Fos 表达是激活神经元的标记,本研究结果表明超重力激活了杏仁核,而给予 7% NaHCO3 可以抑制超重力引起的杏仁核激活。超重力会扰乱空间定向,从而产生晕动病,而杏仁核参与了恐惧反应。最近,Ziemann 等人认为,诱发恐惧的刺激会降低杏仁核的 pH 值,从而激活杏仁核,导致诱发恐惧行为,而施用 HCO3- 可减轻恐惧行为[Cell 2009; 139: 1012-1021]。因此,可能是超重力降低了杏仁核的 pH 值,激活了杏仁核,从而诱发了与晕动病有关的恐惧,而施用 7% 的 NaHCO3 会增加大脑 pH 值,从而抑制超重力诱发的杏仁核激活,抑制与晕动病有关的恐惧。对眩晕症患者而言,7% NaHCO3疗法可提高大脑pH值,从而抑制杏仁核的激活,抑制与眩晕症相关的恐惧感,从而产生有益的临床效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of sodium bicarbonate solution on hypergravity-induced Fos expression in neurons of the amygdala in rats: Implication of sodium bicarbonate therapy for vertigo

Objective

In Japan, intravenous injection of a 7 % solution of sodium bicarbonate (NaHCO3) had been originally developed to inhibit motion sickness and then have long been used to treat vertigo. Previously, we reported that Fos-positive neurons appear in the amygdala after hypergravity stimulation in rats. In the present study, we examined whether injection of 7 % NaHCO3 inhibits hypergravity-induced Fos expression in the neurons in the central nucleus of the amygdala in rats.

Methods

Rats were exposed to 2 G hypergravity in an animal centrifuge device for 3 h. A solution of 7 % NaHCO3 at a dose of 4 mM/kg was injected intraperitoneally before 2 G hypergraviy. Fos-positive neurons in the amygdala were stained immunohistochemically.

Results

The number of Fos-positive neurons in the central nucleus of the amygdala was significantly increased after 2 G hypergravity in rats that received no drugs or saline, compared to that in rats exposed only to the noise of the centrifuge and received 7 % NaHCO3 solution. The number of Fos-positive neurons in the central nucleus of the amygdala after 2 G hypergravity was significantly decreased in rats that received 7 % NaHCO3 solution, compared to that in rats that received no drugs or saline.

Conclusion

Since Fos expression is a marker of activated neurons, the present findings suggest that hypergravity activates the amygdala and that administration of 7 % NaHCO3 suppresses hypergravity-induced activation of the amygdala. Hypergravity disturbs spatial orientation to produce motion sickness and the amygdala is involved in fear response. Recently, Ziemann et al. suggested that fear-evoking stimuli reduce the pH in the amygdala to activate it, leading to induction of fear behavior and that administering HCO3 attenuates fear behavior [Cell 2009; 139: 1012–1021]. Therefore, it is possible that hypergravity reduces the pH in the amygdala to activate it, thereby inducing the fear associated with motion sickness and that administration of 7 % NaHCO3 increases the brain pH thereby suppressing hypergravity-induced activation of the amygdala and inhibiting the fear associated with motion sickness. In patients with vertigo, 7 % NaHCO3 therapy may increase the brain pH thereby suppressing the activation of the amygdala and inhibiting the fear associated with vertigo to elicit a beneficial clinical effect.

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来源期刊
Auris Nasus Larynx
Auris Nasus Larynx 医学-耳鼻喉科学
CiteScore
3.40
自引率
5.90%
发文量
169
审稿时长
30 days
期刊介绍: The international journal Auris Nasus Larynx provides the opportunity for rapid, carefully reviewed publications concerning the fundamental and clinical aspects of otorhinolaryngology and related fields. This includes otology, neurotology, bronchoesophagology, laryngology, rhinology, allergology, head and neck medicine and oncologic surgery, maxillofacial and plastic surgery, audiology, speech science. Original papers, short communications and original case reports can be submitted. Reviews on recent developments are invited regularly and Letters to the Editor commenting on papers or any aspect of Auris Nasus Larynx are welcomed. Founded in 1973 and previously published by the Society for Promotion of International Otorhinolaryngology, the journal is now the official English-language journal of the Oto-Rhino-Laryngological Society of Japan, Inc. The aim of its new international Editorial Board is to make Auris Nasus Larynx an international forum for high quality research and clinical sciences.
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