使用含白藜芦醇的口崩胶囊后珐琅质表层和唾液中白藜芦醇含量的体内变化

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Flávia Mauad Levy , João Victor Frazão Câmara , Talita Mendes Oliveira Ventura , Vinícius Taioqui Pelá , Flávia Iano , Tamara Teodoro Araujo , Thamyris de Souza Carvalho , Nathalia Mariana Pavan , Valdecir Farias Ximenes , Marília Afonso Rabelo Buzalaf
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引用次数: 0

摘要

目的在体内评估 1)反式白藜芦醇通过舌下含服胶囊给药时的生物利用度;2)白藜芦醇对获得性釉质小体(AEP)蛋白质组成的影响。然后在 0、30、60 和 120 分钟后收集未刺激唾液,并在服用胶囊 120 分钟后收集后天性釉质微粒。接下来的一周,志愿者舌下含服安慰剂胶囊,并再次收集唾液和 AEP。采用高效液相色谱法(HPLC)对唾液样本进行游离反式白藜芦醇分析,并对 AEP 样品进行蛋白质组学分析(nLC-ESI-MS/MS)。两组共鉴定出 242 种蛋白质。白藜芦醇组有 96 个蛋白质增加,23 个蛋白质减少。在上调的蛋白质中,白藜芦醇组中胱氨酸、PRPs、粘蛋白-7、组蛋白-1、乳铁蛋白和溶菌酶-C 的同工酶增加,而蛋白 S100、中性粒细胞防御素、白蛋白、PRPs 和 Statherin 的同工酶减少。研究发现,有几种蛋白质参与了维持全身和口腔健康平衡的重要过程。这些因反式白藜芦醇的存在而表达不同的蛋白质值得今后的研究关注,因为它们具有重要的功能,主要与抗菌作用有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vivo modification of the enamel pellicle and saliva resveratrol levels after use of resveratrol-containing orodispersible capsules

Objectives

To evaluate in vivo 1) the bioavailability of trans-resveratrol when administered through sublingual capsules; 2) the effect of resveratrol on the protein composition of the acquired enamel pellicle (AEP).

Design

Ten volunteers received a sublingual capsule containing 50 mg of trans-resveratrol. Unstimulated saliva was then collected after 0, 30, 60, and 120 min and AEP was collected after 120 min following administration of the capsule. In the next week, the volunteers received a placebo sublingual capsule, and saliva and AEP were collected again. Saliva samples were analyzed for free trans-resveratrol using high-performance liquid chromatopgraphy (HPLC), and AEP samples were subjected to proteomic analysis (nLC-ESI-MS/MS).

Results

Trans-resveratrol was detected in saliva at all the time points evaluated, with the peak at 30 min. A total of 242 proteins were identified in both groups. Ninety-six proteins were increased and 23 proteins were decreased in the Resveratrol group. Among the up-regulated proteins, isoforms of cystatins, PRPs, Mucin-7, Histatin-1, Lactotrasnferrin and Lysozyme-C were increased and the isoforms of Protein S100, Neutrophil defensins, Albumin, PRPs, and, Statherin were decreased in Resveratrol group.

Conclusion

The sublingual capsule is effective at increasing the bioavailability of trans-resveratrol in saliva. Several proteins involved in important processes to maintain systemic and oral health homeostasis were identified. These proteins differently expressed due to the presence of trans-resveratrol deserve attention for future studies, since they have important functions, mainly related to antimicrobial action.

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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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