对六种甲壳类动物的肠道成分进行代谢编码可提供详细的饮食描述,并深入了解它们作为捕食者和清道夫的角色

IF 2.1 4区 地球科学 Q2 MARINE & FRESHWATER BIOLOGY
Pauline Boët , Dorothée Kopp , Sonia Méhault , Marie Morfin , Aurélie Bonin , Benjamin Lejeune
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引用次数: 0

摘要

尽管许多研究采用传统的基于形态学的方法分析了海洋消费者的食性,但对许多具有重要功能的物种群(如底栖无脊椎动物)的整体食性仍然缺乏了解。这阻碍了我们精确建立物种间营养联系的能力,而这对于了解食物网和生态系统动态是非常必要的。在这里,我们应用 DNA 代谢编码技术,获得了比斯开湾六种甲壳类动物(食用蟹 Cancer pagurus、绒蟹 Necora puber、圆蟹 Atelecyclus undecimdentatus、蜘蛛蟹 Maja brachydactyla、对虾 Crangon crangon 和寄居蟹 Pagurus bernhardus)食物中动物部分的高分辨率图像。我们使用两对针对 18S rDNA(通性)和 12S rDNA(鱼类特异性)区域的引物来识别构成食性动物部分的猎物。根据出现数据,确定了属于 5 个不同门类的 54 个猎物类群,主要以长鳍鱼类和桡足类为代表。通过对猎物的高水平检测,结果表明所有消费者都采取了清扫策略,主要是食用可能来自于捕捞弃置物的猎物。这项研究还揭示了消费者之间的生态位重叠模式,将 C. crangon 与其他消费者区分开来,并表明 N. puber 和 M. brachydactyla 以及 C. pagurus 在动物资源利用方面存在高度重叠。高分辨率的饮食描述揭示了局部范围内营养相互作用的复杂性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers

While many studies have analyzed the diet of marine consumers using traditional morphology-based methods, the integral diet of many species of functionally important groups such as benthic invertebrates remains poorly resolved. This hinders our ability to precisely establish trophic links among species, which is necessary to understand food webs and ecosystem dynamics. Here, we applied DNA metabarcoding to obtain a high resolution picture of the animal fraction of the diet of six species of crustaceans in the Bay of Biscay; the edible crab Cancer pagurus, the velvet crab Necora puber, the circular crab Atelecyclus undecimdentatus, the spider crab Maja brachydactyla, the shrimp Crangon crangon, and the hermit crab Pagurus bernhardus. We used two pairs of primers targeting the 18S rDNA (generalist) and 12S rDNA (fish-specific) regions to identify prey that makes up the animal fraction of the diet. Based on occurrence data, 54 prey taxa belonging to 5 different phylum mostly represented by teleost fishes and malacostracans were identified. Through a high-level of prey detection, results indicate a scavenging strategy across all consumers mostly based on the consumption of preys likely originating from fishing discards. This study also reveals patterns of niche overlaps among consumers differentiating C. crangon from others and suggesting high overlap in animal resource use between N. puber and M. brachydactyla as well as C. pagurus. High resolution diet description revealed the complexity of trophic interactions on a local scale.

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来源期刊
Journal of Sea Research
Journal of Sea Research 地学-海洋学
CiteScore
3.20
自引率
5.00%
发文量
86
审稿时长
6-12 weeks
期刊介绍: The Journal of Sea Research is an international and multidisciplinary periodical on marine research, with an emphasis on the functioning of marine ecosystems in coastal and shelf seas, including intertidal, estuarine and brackish environments. As several subdisciplines add to this aim, manuscripts are welcome from the fields of marine biology, marine chemistry, marine sedimentology and physical oceanography, provided they add to the understanding of ecosystem processes.
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