羊水γ-谷氨酰转移酶用于预测胎儿胆囊未显影情况下的胆道闭锁:一项使用经验证的分析平台和本地参考范围的回顾性研究。

IF 3.1 4区 医学 Q1 MEDICINE, GENERAL & INTERNAL
Hong Kong Medical Journal Pub Date : 2024-06-01 Epub Date: 2024-06-05 DOI:10.12809/hkmj2210567
T Y T Cheung, N K L Wong, D S Sahota, S R Subramaniam, S L Lau, X Zhu, W T Lui, E K W Chan, Y K Y Kwok, K W Choy, T Y Leung, M H M Chan, F C K Wong, Y H Ting
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引用次数: 0

摘要

导言:羊水中的γ-谷氨酰转移酶(AFGGT)水平有助于鉴别胎儿胆囊未显影(NVFGB)病例中的胆道闭锁(BA)。本研究旨在验证基于血清/血浆基质的羊水(AF)样本γ-谷氨酰转移酶(GGT)检测方法,建立本地孕龄特异性AFGGT参考范围,并评估AFGGT在使用构建的参考范围预测NVFGB孕妇胎儿胆道闭锁的有效性:使用 Cobas c502 分析仪评估了基于血清/血浆基质的羊水样本 GGT 分析仪的分析性能。使用同一台分析仪测定已确诊的单胎妊娠(妊娠16+0周至22+6周)羊水γ-谷氨酰转移酶水平,以确定当地孕龄特异性参考范围(2.5%至97.5%百分位数)。该本地参考范围用于确定 AFGGT 水平的阳性预测值 (PPV) 和阴性预测值 (NPV):基于血清/血浆基质的 GGT 检测能可靠、准确地测定房颤样本中的 GGT 水平。使用构建的本地孕龄特异性 AFGGT 参考范围,AFGGT 水平的 NPV 和 PPV 均为 0.1:在 NVFGB 孕妇中,AFGGT 水平≥2.5 百分位数可能排除胎儿 BA。虽然 AFGGT 水平
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amniotic fluid gamma-glutamyl transferase for prediction of biliary atresia in cases of non-visualisation of the fetal gallbladder: a retrospective study using a validated analytical platform and local reference range.

Introduction: The level of amniotic fluid gamma-glutamyl transferase (AFGGT) may help identify biliary atresia (BA) in cases of non-visualisation of the fetal gallbladder (NVFGB). This study aimed to validate a serum/plasma matrix-based gamma-glutamyl transferase (GGT) assay for amniotic fluid (AF) samples, establish a local gestational age-specific AFGGT reference range, and evaluate the efficacy of AFGGT for predicting fetal BA in pregnancies with NVFGB using the constructed reference range.

Methods: The analytical performance of a serum/plasma matrix-based GGT assay on AF samples was evaluated using a Cobas c502 analyser. Amniotic fluid gamma-glutamyl transferase levels in confirmed euploid singleton pregnancies (16+0 to 22+6 weeks of gestation) were determined using the same analyser to establish a local gestational age-specific reference range (the 2.5th to 97.5th percentiles). This local reference range was used to determine the positive predictive value (PPV) and negative predictive value (NPV) of AFGGT level <2.5th percentile for identifying fetal BA in euploid pregnancies with NVFGB.

Results: The serum/plasma matrix-based GGT assay was able to reliably and accurately determine GGT levels in AF samples. Using the constructed local gestational age-specific AFGGT reference range, the NPV and PPV of AFGGT level <2.5th percentile for predicting fetal BA in pregnancies with NVFGB were 100% and 25% (95% confidence interval=0, 53), respectively.

Conclusion: In pregnancies with NVFGB, AFGGT level ≥2.5th percentile likely excludes fetal BA. Although AFGGT level <2.5th percentile is not diagnostic of fetal BA, fetuses with AFGGT below this level should be referred for early postnatal investigation.

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来源期刊
Hong Kong Medical Journal
Hong Kong Medical Journal MEDICINE, GENERAL & INTERNAL-
CiteScore
1.50
自引率
14.80%
发文量
117
审稿时长
10 weeks
期刊介绍: The HKMJ is a Hong Kong-based, peer-reviewed, general medical journal which is circulated to 6000 readers, including all members of the HKMA and Fellows of the HKAM. The HKMJ publishes original research papers, review articles, medical practice papers, case reports, editorials, commentaries, book reviews, and letters to the Editor. Topics of interest include all subjects that relate to clinical practice and research in all branches of medicine. The HKMJ welcomes manuscripts from authors, but usually solicits reviews. Proposals for review papers can be sent to the Managing Editor directly. Please refer to the contact information of the Editorial Office.
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