头孢菌素水解和抑制金黄色葡萄球菌和大肠杆菌生物膜的效果。

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Jawaria Aslam, Hafiz Muhammad Ali, Shujaat Hussain, Muhammad Zishan Ahmad, Abu Baker Siddique, Muhammad Shahid, Mirza Imran Shahzad, Hina Fatima, Sarah Tariq, Fatima Sadiq, Maria Aslam, Umar Farooq, Saadiya Zia, Rawa Saad Aljaluod, Khaloud Mohammed Alarjani
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引用次数: 0

摘要

重要性:金黄色葡萄球菌和大肠埃希菌通过形成生物膜应对全球健康挑战,生物膜是一种关键的毒力要素,与多种感染的发病机制有关:本研究考察了各代头孢菌素对致病性金黄色葡萄球菌和大肠杆菌形成的生物膜的疗效:方法:采用培养皿法和微孔板法评估两种细菌形成生物膜的情况。使用第一代至第四代头孢菌素测试生物膜水解和抑制作用,并通过水晶紫染色和相衬显微镜分析其效果:结果:在培养皿和微孔板试验中,两种细菌菌株都表现出发达的生物膜。头孢拉定(第一代)对金黄色葡萄球菌生物膜的水解率为 76.78%,而头孢吡肟(第四代)对大肠杆菌生物膜的水解率为 59.86%。同样,头孢呋辛、头孢羟氨苄、头孢吡肟和头孢拉定对金黄色葡萄球菌生物膜的抑制率分别为 78.8%、71.63%、70.63% 和 70.51%。头孢吡肟对大肠杆菌生物膜的抑制率(66.47%)也最高。所有几代头孢菌素对金黄色葡萄球菌的抑制效果均优于对大肠杆菌的抑制效果,相衬显微镜证实了这一点:头孢菌素具有水解和抑制金黄色葡萄球菌和大肠杆菌生物膜的双重能力。第一代头孢菌素对金黄色葡萄球菌的抑制活性最高,而第三代和第四代头孢菌素则能显著抑制大肠杆菌生物膜。这项研究强调了根据特定细菌菌株的生物膜特征制定针对性抗生素策略的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effectiveness of cephalosporins in hydrolysis and inhibition of Staphylococcus aureus and Escherichia coli biofilms.

Importance: Staphylococcus aureus and Escherichia coli contribute to global health challenges by forming biofilms, a key virulence element implicated in the pathogenesis of several infections.

Objective: The study examined the efficacy of various generations of cephalosporins against biofilms developed by pathogenic S. aureus and E. coli.

Methods: The development of biofilms by both bacteria was assessed using petri-plate and microplate methods. Biofilm hydrolysis and inhibition were tested using first to fourth generations of cephalosporins, and the effects were analyzed by crystal violet staining and phase contrast microscopy.

Results: Both bacterial strains exhibited well-developed biofilms in petri-plate and microplate assays. Cefradine (first generation) showed 76.78% hydrolysis of S. aureus biofilm, while significant hydrolysis (59.86%) of E. coli biofilm was observed by cefipime (fourth generation). Similarly, cefuroxime, cefadroxil, cefepime, and cefradine caused 78.8%, 71.63%, 70.63%, and 70.51% inhibition of the S. aureus biofilms, respectively. In the case of E. coli, maximum biofilm inhibition (66.47%) was again shown by cefepime. All generations of cephalosporins were more effective against S. aureus than E. coli, which was confirmed by phase contrast microscopy.

Conclusions and relevance: Cephalosporins exhibit dual capabilities of hydrolyzing and inhibiting S. aureus and E. coli biofilms. First-generation cephalosporins exhibited the highest inhibitory activity against S. aureus, while the third and fourth generations significantly inhibited E. coli biofilms. This study highlights the importance of tailored antibiotic strategies based on the biofilm characteristics of specific bacterial strains.

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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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