Jawaria Aslam, Hafiz Muhammad Ali, Shujaat Hussain, Muhammad Zishan Ahmad, Abu Baker Siddique, Muhammad Shahid, Mirza Imran Shahzad, Hina Fatima, Sarah Tariq, Fatima Sadiq, Maria Aslam, Umar Farooq, Saadiya Zia, Rawa Saad Aljaluod, Khaloud Mohammed Alarjani
{"title":"头孢菌素水解和抑制金黄色葡萄球菌和大肠杆菌生物膜的效果。","authors":"Jawaria Aslam, Hafiz Muhammad Ali, Shujaat Hussain, Muhammad Zishan Ahmad, Abu Baker Siddique, Muhammad Shahid, Mirza Imran Shahzad, Hina Fatima, Sarah Tariq, Fatima Sadiq, Maria Aslam, Umar Farooq, Saadiya Zia, Rawa Saad Aljaluod, Khaloud Mohammed Alarjani","doi":"10.4142/jvs.23258","DOIUrl":null,"url":null,"abstract":"<p><strong>Importance: </strong><i>Staphylococcus aureus</i> and <i>Escherichia coli</i> contribute to global health challenges by forming biofilms, a key virulence element implicated in the pathogenesis of several infections.</p><p><strong>Objective: </strong>The study examined the efficacy of various generations of cephalosporins against biofilms developed by pathogenic <i>S. aureus</i> and <i>E. coli</i>.</p><p><strong>Methods: </strong>The development of biofilms by both bacteria was assessed using petri-plate and microplate methods. Biofilm hydrolysis and inhibition were tested using first to fourth generations of cephalosporins, and the effects were analyzed by crystal violet staining and phase contrast microscopy.</p><p><strong>Results: </strong>Both bacterial strains exhibited well-developed biofilms in petri-plate and microplate assays. Cefradine (first generation) showed 76.78% hydrolysis of <i>S. aureus</i> biofilm, while significant hydrolysis (59.86%) of <i>E. coli</i> biofilm was observed by cefipime (fourth generation). Similarly, cefuroxime, cefadroxil, cefepime, and cefradine caused 78.8%, 71.63%, 70.63%, and 70.51% inhibition of the <i>S. aureus</i> biofilms, respectively. In the case of <i>E. coli,</i> maximum biofilm inhibition (66.47%) was again shown by cefepime. All generations of cephalosporins were more effective against <i>S. aureus</i> than <i>E. coli</i>, which was confirmed by phase contrast microscopy.</p><p><strong>Conclusions and relevance: </strong>Cephalosporins exhibit dual capabilities of hydrolyzing and inhibiting <i>S. aureus</i> and <i>E. coli</i> biofilms. First-generation cephalosporins exhibited the highest inhibitory activity against <i>S. aureus</i>, while the third and fourth generations significantly inhibited <i>E. coli</i> biofilms. This study highlights the importance of tailored antibiotic strategies based on the biofilm characteristics of specific bacterial strains.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e47"},"PeriodicalIF":1.5000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156599/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effectiveness of cephalosporins in hydrolysis and inhibition of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> biofilms.\",\"authors\":\"Jawaria Aslam, Hafiz Muhammad Ali, Shujaat Hussain, Muhammad Zishan Ahmad, Abu Baker Siddique, Muhammad Shahid, Mirza Imran Shahzad, Hina Fatima, Sarah Tariq, Fatima Sadiq, Maria Aslam, Umar Farooq, Saadiya Zia, Rawa Saad Aljaluod, Khaloud Mohammed Alarjani\",\"doi\":\"10.4142/jvs.23258\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Importance: </strong><i>Staphylococcus aureus</i> and <i>Escherichia coli</i> contribute to global health challenges by forming biofilms, a key virulence element implicated in the pathogenesis of several infections.</p><p><strong>Objective: </strong>The study examined the efficacy of various generations of cephalosporins against biofilms developed by pathogenic <i>S. aureus</i> and <i>E. coli</i>.</p><p><strong>Methods: </strong>The development of biofilms by both bacteria was assessed using petri-plate and microplate methods. Biofilm hydrolysis and inhibition were tested using first to fourth generations of cephalosporins, and the effects were analyzed by crystal violet staining and phase contrast microscopy.</p><p><strong>Results: </strong>Both bacterial strains exhibited well-developed biofilms in petri-plate and microplate assays. Cefradine (first generation) showed 76.78% hydrolysis of <i>S. aureus</i> biofilm, while significant hydrolysis (59.86%) of <i>E. coli</i> biofilm was observed by cefipime (fourth generation). Similarly, cefuroxime, cefadroxil, cefepime, and cefradine caused 78.8%, 71.63%, 70.63%, and 70.51% inhibition of the <i>S. aureus</i> biofilms, respectively. In the case of <i>E. coli,</i> maximum biofilm inhibition (66.47%) was again shown by cefepime. All generations of cephalosporins were more effective against <i>S. aureus</i> than <i>E. coli</i>, which was confirmed by phase contrast microscopy.</p><p><strong>Conclusions and relevance: </strong>Cephalosporins exhibit dual capabilities of hydrolyzing and inhibiting <i>S. aureus</i> and <i>E. coli</i> biofilms. First-generation cephalosporins exhibited the highest inhibitory activity against <i>S. aureus</i>, while the third and fourth generations significantly inhibited <i>E. coli</i> biofilms. This study highlights the importance of tailored antibiotic strategies based on the biofilm characteristics of specific bacterial strains.</p>\",\"PeriodicalId\":17557,\"journal\":{\"name\":\"Journal of Veterinary Science\",\"volume\":\"25 3\",\"pages\":\"e47\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156599/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.4142/jvs.23258\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4142/jvs.23258","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Effectiveness of cephalosporins in hydrolysis and inhibition of Staphylococcus aureus and Escherichia coli biofilms.
Importance: Staphylococcus aureus and Escherichia coli contribute to global health challenges by forming biofilms, a key virulence element implicated in the pathogenesis of several infections.
Objective: The study examined the efficacy of various generations of cephalosporins against biofilms developed by pathogenic S. aureus and E. coli.
Methods: The development of biofilms by both bacteria was assessed using petri-plate and microplate methods. Biofilm hydrolysis and inhibition were tested using first to fourth generations of cephalosporins, and the effects were analyzed by crystal violet staining and phase contrast microscopy.
Results: Both bacterial strains exhibited well-developed biofilms in petri-plate and microplate assays. Cefradine (first generation) showed 76.78% hydrolysis of S. aureus biofilm, while significant hydrolysis (59.86%) of E. coli biofilm was observed by cefipime (fourth generation). Similarly, cefuroxime, cefadroxil, cefepime, and cefradine caused 78.8%, 71.63%, 70.63%, and 70.51% inhibition of the S. aureus biofilms, respectively. In the case of E. coli, maximum biofilm inhibition (66.47%) was again shown by cefepime. All generations of cephalosporins were more effective against S. aureus than E. coli, which was confirmed by phase contrast microscopy.
Conclusions and relevance: Cephalosporins exhibit dual capabilities of hydrolyzing and inhibiting S. aureus and E. coli biofilms. First-generation cephalosporins exhibited the highest inhibitory activity against S. aureus, while the third and fourth generations significantly inhibited E. coli biofilms. This study highlights the importance of tailored antibiotic strategies based on the biofilm characteristics of specific bacterial strains.
期刊介绍:
The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.