{"title":"基于纳米氧化钨（VI）吸附响应表面增强光致变色现象的氨基酸无标记比色分析策略。","authors":"Shohei Tanaka, Kenta Adachi","doi":"10.1007/s44211-024-00607-6","DOIUrl":null,"url":null,"abstract":"<div><p>Herein, we present a colorimetric detection method based on the surface-enhanced photochromic phenomenon of tungsten (VI) oxide (WO<sub>3</sub>) nanocolloid particles for α-amino acid (AA) molecules, including <sub>L</sub>-aspartic acid (Asp), <sub>L</sub>-glutamic acid (Glu), <sub>L</sub>-histidine (His), <sub>L</sub>-isoleucine (Ile), <sub>L</sub>-leucine (Leu), <sub>L</sub>-lysine (Lys), <sub>L</sub>-phenylalanine (Phe), and <sub>L</sub>-valine (Val). The UV-induced photochromic phenomena in the AA/WO<sub>3</sub> binary aqueous systems were investigated using UV–Vis absorption spectrometry. The adsorption properties of the AA molecules on the surface of the WO<sub>3</sub> nanocolloid particles have been identified using a combination of adsorption isotherm analysis and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. A good linear correlation between the concentration of the AAs adsorbed on the surface of the WO<sub>3</sub> nanocolloid particles and the initial photochromic coloration rate in the corresponding UV-irradiated WO<sub>3</sub> colloidal aqueous solution was obtained with over three orders of magnitude, indicating that the surface-enhanced photochromic phenomenon of the WO<sub>3</sub> nanocolloid particle can be used to detect the AA molecules. In addition, based on the results of the UV–Vis absorption, ATR-FTIR, and adsorption isotherm analyses, we have experimentally demonstrated that the AA/WO<sub>3</sub> binary aqueous system with inner-sphere adsorbed Ile, Leu, Lys, or Val molecules on the surface of the WO<sub>3</sub> nanocolloid particles exhibits a more significant surface-enhanced photochromic phenomenon than the system with outer-sphere adsorbed Asp, Glu, His, or Phe molecules. The strong inner-sphere adsorption of the AA molecules successfully improved the limit of detection. This study provides valuable insights into a “label-free” colorimetric assay system based on the surface-enhanced photochromic phenomenon of the WO<sub>3</sub> nanocolloid probe.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Label-free colorimetric analysis strategies based on adsorption-responsive surface-enhanced photochromic phenomena of tungsten(VI) oxide nanoparticles for amino acids\",\"authors\":\"Shohei Tanaka, Kenta Adachi\",\"doi\":\"10.1007/s44211-024-00607-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Herein, we present a colorimetric detection method based on the surface-enhanced photochromic phenomenon of tungsten (VI) oxide (WO<sub>3</sub>) nanocolloid particles for α-amino acid (AA) molecules, including <sub>L</sub>-aspartic acid (Asp), <sub>L</sub>-glutamic acid (Glu), <sub>L</sub>-histidine (His), <sub>L</sub>-isoleucine (Ile), <sub>L</sub>-leucine (Leu), <sub>L</sub>-lysine (Lys), <sub>L</sub>-phenylalanine (Phe), and <sub>L</sub>-valine (Val). The UV-induced photochromic phenomena in the AA/WO<sub>3</sub> binary aqueous systems were investigated using UV–Vis absorption spectrometry. The adsorption properties of the AA molecules on the surface of the WO<sub>3</sub> nanocolloid particles have been identified using a combination of adsorption isotherm analysis and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. A good linear correlation between the concentration of the AAs adsorbed on the surface of the WO<sub>3</sub> nanocolloid particles and the initial photochromic coloration rate in the corresponding UV-irradiated WO<sub>3</sub> colloidal aqueous solution was obtained with over three orders of magnitude, indicating that the surface-enhanced photochromic phenomenon of the WO<sub>3</sub> nanocolloid particle can be used to detect the AA molecules. In addition, based on the results of the UV–Vis absorption, ATR-FTIR, and adsorption isotherm analyses, we have experimentally demonstrated that the AA/WO<sub>3</sub> binary aqueous system with inner-sphere adsorbed Ile, Leu, Lys, or Val molecules on the surface of the WO<sub>3</sub> nanocolloid particles exhibits a more significant surface-enhanced photochromic phenomenon than the system with outer-sphere adsorbed Asp, Glu, His, or Phe molecules. The strong inner-sphere adsorption of the AA molecules successfully improved the limit of detection. 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引用次数: 0
摘要
在此，我们提出了一种基于纳米氧化钨（VI）（WO3）颗粒表面增强光致变色现象的α-氨基酸（AA）分子比色检测方法、包括 L-天冬氨酸（Asp）、L-谷氨酸（Glu）、L-组氨酸（His）、L-异亮氨酸（Ile）、L-亮氨酸（Leu）、L-赖氨酸（Lys）、L-苯丙氨酸（Phe）和 L-缬氨酸（Val）。利用紫外可见吸收光谱法研究了 AA/WO3 二元水体系中紫外线诱导的光致变色现象。利用吸附等温线分析和衰减全反射傅立叶变换红外光谱（ATR-FTIR）相结合的方法，确定了 AA 分子在 WO3 纳米胶体颗粒表面的吸附特性。结果表明，WO3 纳米胶体颗粒表面吸附的 AAs 浓度与相应的经紫外光照射的 WO3 胶体水溶液中的初始光致变色率之间具有良好的线性关系，相关性超过三个数量级，表明 WO3 纳米胶体颗粒的表面增强光致变色现象可用于检测 AA 分子。此外，根据紫外-可见吸收、ATR-傅立叶变换红外光谱和吸附等温线分析的结果，我们通过实验证明了 WO3 纳米胶体粒子表面内球吸附有 Ile、Leu、Lys 或 Val 分子的 AA/WO3 二元水体系比外球吸附有 Asp、Glu、His 或 Phe 分子的体系表现出更明显的表面增强光致变色现象。内球对 AA 分子的强吸附成功地提高了检测限。这项研究为基于 WO3 纳米胶体探针的表面增强光致变色现象的 "无标记 "比色检测系统提供了宝贵的见解。
Label-free colorimetric analysis strategies based on adsorption-responsive surface-enhanced photochromic phenomena of tungsten(VI) oxide nanoparticles for amino acids
Herein, we present a colorimetric detection method based on the surface-enhanced photochromic phenomenon of tungsten (VI) oxide (WO3) nanocolloid particles for α-amino acid (AA) molecules, including L-aspartic acid (Asp), L-glutamic acid (Glu), L-histidine (His), L-isoleucine (Ile), L-leucine (Leu), L-lysine (Lys), L-phenylalanine (Phe), and L-valine (Val). The UV-induced photochromic phenomena in the AA/WO3 binary aqueous systems were investigated using UV–Vis absorption spectrometry. The adsorption properties of the AA molecules on the surface of the WO3 nanocolloid particles have been identified using a combination of adsorption isotherm analysis and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. A good linear correlation between the concentration of the AAs adsorbed on the surface of the WO3 nanocolloid particles and the initial photochromic coloration rate in the corresponding UV-irradiated WO3 colloidal aqueous solution was obtained with over three orders of magnitude, indicating that the surface-enhanced photochromic phenomenon of the WO3 nanocolloid particle can be used to detect the AA molecules. In addition, based on the results of the UV–Vis absorption, ATR-FTIR, and adsorption isotherm analyses, we have experimentally demonstrated that the AA/WO3 binary aqueous system with inner-sphere adsorbed Ile, Leu, Lys, or Val molecules on the surface of the WO3 nanocolloid particles exhibits a more significant surface-enhanced photochromic phenomenon than the system with outer-sphere adsorbed Asp, Glu, His, or Phe molecules. The strong inner-sphere adsorption of the AA molecules successfully improved the limit of detection. This study provides valuable insights into a “label-free” colorimetric assay system based on the surface-enhanced photochromic phenomenon of the WO3 nanocolloid probe.
期刊介绍：
Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods.
This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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