{"title":"2012 年至 2020 年期间在墨西哥西北部沿海地区采集的牡蛎中副溶血性弧菌的血清多样性、抗生素耐药性和毒力基因","authors":"Hector Flores-Villaseñor , Jorge Velázquez-Román , Nidia León-Sicairos , Uriel A. Angulo-Zamudio , Carolina Lira-Morales , Jesús J. Martínez-García , Erika Acosta-Smith , Jorge Valdés-Flores , Gabriela Tapia-Pastrana , Adrian Canizalez-Román","doi":"10.1016/j.fm.2024.104567","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to determine the prevalence of <em>V. parahaemolyticus</em> in oysters from the northwestern coast of Mexico and to identify the serotypes, virulence factors, and antibiotic resistance of the strains. Oyster samples were collected from 2012 to 2020 from the northwest coast of Mexico; biochemical and molecular methods were used to identify <em>V. parahaemolyticus</em> from oysters; antiserum reaction to determine <em>V. parahaemolyticus</em> serotypes, and PCR assays were performed to identify pathogenic (<em>tdh</em> and/or <em>trh</em>) or pandemic (<em>toxRS/new</em>, and/or <em>orf8</em>) strains and antibiotic resistance testing. A total of 441 oyster samples were collected and tested for <em>V. parahaemolyticus</em>. Forty-seven percent of oyster samples were positive for <em>V. parahaemolyticus</em>. Ten different O serogroups and 72 serovars were identified, predominantly serotype O1:KUT with 22.2% and OUT:KUT with 17.3%. Twenty new serotypes that had not been previously reported in our region were identified. We detected 4.3% of pathogenic clones but no pandemic strains. About 73.5% of strains were resistant to at least one antibiotic, mainly ampicillin and ciprofloxacin; 25% were multi-drug resistant. In conclusion, the pathogenic strains in oysters and antibiotic resistance are of public health concern, as the potential for outbreaks throughout northwestern Mexico is well established.</p></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"123 ","pages":"Article 104567"},"PeriodicalIF":4.5000,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Serodiversity, antibiotic resistance, and virulence genes of Vibrio parahaemolyticus in oysters collected in coastal areas of northwestern Mexico between 2012 and 2020\",\"authors\":\"Hector Flores-Villaseñor , Jorge Velázquez-Román , Nidia León-Sicairos , Uriel A. Angulo-Zamudio , Carolina Lira-Morales , Jesús J. Martínez-García , Erika Acosta-Smith , Jorge Valdés-Flores , Gabriela Tapia-Pastrana , Adrian Canizalez-Román\",\"doi\":\"10.1016/j.fm.2024.104567\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to determine the prevalence of <em>V. parahaemolyticus</em> in oysters from the northwestern coast of Mexico and to identify the serotypes, virulence factors, and antibiotic resistance of the strains. Oyster samples were collected from 2012 to 2020 from the northwest coast of Mexico; biochemical and molecular methods were used to identify <em>V. parahaemolyticus</em> from oysters; antiserum reaction to determine <em>V. parahaemolyticus</em> serotypes, and PCR assays were performed to identify pathogenic (<em>tdh</em> and/or <em>trh</em>) or pandemic (<em>toxRS/new</em>, and/or <em>orf8</em>) strains and antibiotic resistance testing. A total of 441 oyster samples were collected and tested for <em>V. parahaemolyticus</em>. Forty-seven percent of oyster samples were positive for <em>V. parahaemolyticus</em>. Ten different O serogroups and 72 serovars were identified, predominantly serotype O1:KUT with 22.2% and OUT:KUT with 17.3%. Twenty new serotypes that had not been previously reported in our region were identified. We detected 4.3% of pathogenic clones but no pandemic strains. About 73.5% of strains were resistant to at least one antibiotic, mainly ampicillin and ciprofloxacin; 25% were multi-drug resistant. In conclusion, the pathogenic strains in oysters and antibiotic resistance are of public health concern, as the potential for outbreaks throughout northwestern Mexico is well established.</p></div>\",\"PeriodicalId\":12399,\"journal\":{\"name\":\"Food microbiology\",\"volume\":\"123 \",\"pages\":\"Article 104567\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0740002024001059\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0740002024001059","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Serodiversity, antibiotic resistance, and virulence genes of Vibrio parahaemolyticus in oysters collected in coastal areas of northwestern Mexico between 2012 and 2020
This study aimed to determine the prevalence of V. parahaemolyticus in oysters from the northwestern coast of Mexico and to identify the serotypes, virulence factors, and antibiotic resistance of the strains. Oyster samples were collected from 2012 to 2020 from the northwest coast of Mexico; biochemical and molecular methods were used to identify V. parahaemolyticus from oysters; antiserum reaction to determine V. parahaemolyticus serotypes, and PCR assays were performed to identify pathogenic (tdh and/or trh) or pandemic (toxRS/new, and/or orf8) strains and antibiotic resistance testing. A total of 441 oyster samples were collected and tested for V. parahaemolyticus. Forty-seven percent of oyster samples were positive for V. parahaemolyticus. Ten different O serogroups and 72 serovars were identified, predominantly serotype O1:KUT with 22.2% and OUT:KUT with 17.3%. Twenty new serotypes that had not been previously reported in our region were identified. We detected 4.3% of pathogenic clones but no pandemic strains. About 73.5% of strains were resistant to at least one antibiotic, mainly ampicillin and ciprofloxacin; 25% were multi-drug resistant. In conclusion, the pathogenic strains in oysters and antibiotic resistance are of public health concern, as the potential for outbreaks throughout northwestern Mexico is well established.
期刊介绍:
Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.