2012 年至 2020 年期间在墨西哥西北部沿海地区采集的牡蛎中副溶血性弧菌的血清多样性、抗生素耐药性和毒力基因

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hector Flores-Villaseñor , Jorge Velázquez-Román , Nidia León-Sicairos , Uriel A. Angulo-Zamudio , Carolina Lira-Morales , Jesús J. Martínez-García , Erika Acosta-Smith , Jorge Valdés-Flores , Gabriela Tapia-Pastrana , Adrian Canizalez-Román
{"title":"2012 年至 2020 年期间在墨西哥西北部沿海地区采集的牡蛎中副溶血性弧菌的血清多样性、抗生素耐药性和毒力基因","authors":"Hector Flores-Villaseñor ,&nbsp;Jorge Velázquez-Román ,&nbsp;Nidia León-Sicairos ,&nbsp;Uriel A. Angulo-Zamudio ,&nbsp;Carolina Lira-Morales ,&nbsp;Jesús J. Martínez-García ,&nbsp;Erika Acosta-Smith ,&nbsp;Jorge Valdés-Flores ,&nbsp;Gabriela Tapia-Pastrana ,&nbsp;Adrian Canizalez-Román","doi":"10.1016/j.fm.2024.104567","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to determine the prevalence of <em>V. parahaemolyticus</em> in oysters from the northwestern coast of Mexico and to identify the serotypes, virulence factors, and antibiotic resistance of the strains. Oyster samples were collected from 2012 to 2020 from the northwest coast of Mexico; biochemical and molecular methods were used to identify <em>V. parahaemolyticus</em> from oysters; antiserum reaction to determine <em>V. parahaemolyticus</em> serotypes, and PCR assays were performed to identify pathogenic (<em>tdh</em> and/or <em>trh</em>) or pandemic (<em>toxRS/new</em>, and/or <em>orf8</em>) strains and antibiotic resistance testing. A total of 441 oyster samples were collected and tested for <em>V. parahaemolyticus</em>. Forty-seven percent of oyster samples were positive for <em>V. parahaemolyticus</em>. Ten different O serogroups and 72 serovars were identified, predominantly serotype O1:KUT with 22.2% and OUT:KUT with 17.3%. Twenty new serotypes that had not been previously reported in our region were identified. We detected 4.3% of pathogenic clones but no pandemic strains. About 73.5% of strains were resistant to at least one antibiotic, mainly ampicillin and ciprofloxacin; 25% were multi-drug resistant. In conclusion, the pathogenic strains in oysters and antibiotic resistance are of public health concern, as the potential for outbreaks throughout northwestern Mexico is well established.</p></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"123 ","pages":"Article 104567"},"PeriodicalIF":4.5000,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Serodiversity, antibiotic resistance, and virulence genes of Vibrio parahaemolyticus in oysters collected in coastal areas of northwestern Mexico between 2012 and 2020\",\"authors\":\"Hector Flores-Villaseñor ,&nbsp;Jorge Velázquez-Román ,&nbsp;Nidia León-Sicairos ,&nbsp;Uriel A. Angulo-Zamudio ,&nbsp;Carolina Lira-Morales ,&nbsp;Jesús J. Martínez-García ,&nbsp;Erika Acosta-Smith ,&nbsp;Jorge Valdés-Flores ,&nbsp;Gabriela Tapia-Pastrana ,&nbsp;Adrian Canizalez-Román\",\"doi\":\"10.1016/j.fm.2024.104567\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to determine the prevalence of <em>V. parahaemolyticus</em> in oysters from the northwestern coast of Mexico and to identify the serotypes, virulence factors, and antibiotic resistance of the strains. Oyster samples were collected from 2012 to 2020 from the northwest coast of Mexico; biochemical and molecular methods were used to identify <em>V. parahaemolyticus</em> from oysters; antiserum reaction to determine <em>V. parahaemolyticus</em> serotypes, and PCR assays were performed to identify pathogenic (<em>tdh</em> and/or <em>trh</em>) or pandemic (<em>toxRS/new</em>, and/or <em>orf8</em>) strains and antibiotic resistance testing. A total of 441 oyster samples were collected and tested for <em>V. parahaemolyticus</em>. Forty-seven percent of oyster samples were positive for <em>V. parahaemolyticus</em>. Ten different O serogroups and 72 serovars were identified, predominantly serotype O1:KUT with 22.2% and OUT:KUT with 17.3%. Twenty new serotypes that had not been previously reported in our region were identified. We detected 4.3% of pathogenic clones but no pandemic strains. About 73.5% of strains were resistant to at least one antibiotic, mainly ampicillin and ciprofloxacin; 25% were multi-drug resistant. In conclusion, the pathogenic strains in oysters and antibiotic resistance are of public health concern, as the potential for outbreaks throughout northwestern Mexico is well established.</p></div>\",\"PeriodicalId\":12399,\"journal\":{\"name\":\"Food microbiology\",\"volume\":\"123 \",\"pages\":\"Article 104567\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0740002024001059\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0740002024001059","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在确定墨西哥西北海岸牡蛎中副溶血性弧菌的流行率,并确定菌株的血清型、毒力因子和抗生素耐药性。从2012年至2020年,在墨西哥西北海岸采集了牡蛎样本;采用生化和分子方法鉴定牡蛎中的副溶血性弧菌;通过抗血清反应确定副溶血性弧菌的血清型,并通过PCR检测鉴定致病性(tdh和/或trh)或大流行性(toxRS/new和/或orf8)菌株和抗生素耐药性测试。共收集了 441 份牡蛎样本,并对其进行了副溶血性弧菌检测。47% 的牡蛎样本对副溶血性弧菌呈阳性反应。确定了 10 个不同的 O 型血清群和 72 个血清型,主要是 O1:KUT 血清型(22.2%)和 OUT:KUT 血清型(17.3%)。我们还发现了 20 种以前未在本地区报道过的新血清型。我们发现了 4.3% 的致病克隆,但没有大流行菌株。约 73.5%的菌株对至少一种抗生素具有耐药性,主要是氨苄西林和环丙沙星;25%的菌株具有多重耐药性。总之,牡蛎中的致病菌株和抗生素耐药性是公共卫生关注的问题,因为在墨西哥西北部爆发疫情的可能性已经得到证实。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serodiversity, antibiotic resistance, and virulence genes of Vibrio parahaemolyticus in oysters collected in coastal areas of northwestern Mexico between 2012 and 2020

This study aimed to determine the prevalence of V. parahaemolyticus in oysters from the northwestern coast of Mexico and to identify the serotypes, virulence factors, and antibiotic resistance of the strains. Oyster samples were collected from 2012 to 2020 from the northwest coast of Mexico; biochemical and molecular methods were used to identify V. parahaemolyticus from oysters; antiserum reaction to determine V. parahaemolyticus serotypes, and PCR assays were performed to identify pathogenic (tdh and/or trh) or pandemic (toxRS/new, and/or orf8) strains and antibiotic resistance testing. A total of 441 oyster samples were collected and tested for V. parahaemolyticus. Forty-seven percent of oyster samples were positive for V. parahaemolyticus. Ten different O serogroups and 72 serovars were identified, predominantly serotype O1:KUT with 22.2% and OUT:KUT with 17.3%. Twenty new serotypes that had not been previously reported in our region were identified. We detected 4.3% of pathogenic clones but no pandemic strains. About 73.5% of strains were resistant to at least one antibiotic, mainly ampicillin and ciprofloxacin; 25% were multi-drug resistant. In conclusion, the pathogenic strains in oysters and antibiotic resistance are of public health concern, as the potential for outbreaks throughout northwestern Mexico is well established.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信