用于成像和诊断细菌感染的寡核苷酸探针。

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Luís Moreira, Nuno Miguel Guimarães, Rita Sobral Santos, Joana Angélica Loureiro, Maria do Carmo Pereira, Nuno Filipe Azevedo
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引用次数: 0

摘要

随着传染病成为公共卫生问题,有必要开发快速、精确的诊断方法。核成像和光学成像等成像技术提供了诊断体内传染病的能力,消除了临床样本取样和预富集造成的延误,并提供了有助于做出更明智诊断的空间信息。传统的分子探针通常只能对受感染的组织进行成像,而不能准确识别病原体。相比之下,寡核苷酸可以针对特定的 RNA 序列进行定制,从而识别病原体,甚至通过关注耐药基因生成抗生素敏感性图谱。尽管核酸模拟物(NAMs)在稳定寡核苷酸方面有很多好处,但这些相对较大的分子无法充分输送到细菌的细胞质中,这仍然是广泛使用这项技术的一个挑战。本综述总结了用于体内成像的寡核苷酸探针领域的主要进展,重点介绍了文献中描述的最有希望通过体内杂交发展光学成像的输送系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oligonucleotide probes for imaging and diagnosis of bacterial infections.

The rise of infectious diseases as a public health concern has necessitated the development of rapid and precise diagnostic methods. Imaging techniques like nuclear and optical imaging provide the ability to diagnose infectious diseases within the body, eliminating delays caused by sampling and pre-enrichments of clinical samples and offering spatial information that can aid in a more informed diagnosis. Traditional molecular probes are typically created to image infected tissue without accurately identifying the pathogen. In contrast, oligonucleotides can be tailored to target specific RNA sequences, allowing for the identification of pathogens, and even generating antibiotic susceptibility profiles by focusing on drug resistance genes. Despite the benefits that nucleic acid mimics (NAMs) have provided in terms of stabilizing oligonucleotides, the inadequate delivery of these relatively large molecules into the cytoplasm of bacteria remains a challenge for widespread use of this technology. This review summarizes the key advancements in the field of oligonucleotide probes for in vivo imaging, highlighting the most promising delivery systems described in the literature for developing optical imaging through in vivo hybridization.

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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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