肌肉、活动能力和衰老研究》中,肌肉中线粒体氧化应激反应基因的表达与线粒体呼吸、体能和肌肉质量有关。

IF 7.8 1区 医学 Q1 Biochemistry, Genetics and Molecular Biology
Aging Cell Pub Date : 2024-06-03 DOI:10.1111/acel.14114
Gregory J. Tranah, Haley N. Barnes, Peggy M. Cawthon, Paul M. Coen, Karyn A. Esser, Russell T. Hepple, Zhiguang Huo, Philip A. Kramer, Frederico G. S. Toledo, Xiping Zhang, Kevin Wu, Christopher A. Wolff, Daniel S. Evans, Steven R. Cummings
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引用次数: 0

摘要

老年人骨骼肌中的基因表达可能反映了对氧化损伤的补偿性适应,从而保持组织的完整性并维持功能。确定老年人氧化应激反应基因表达模式与线粒体功能、体能表现和肌肉质量之间的关联,将有助于我们进一步了解与管理分子损伤有关的机制,从而有针对性地保护身体复原能力。为了描述负责氧化应激反应的基因的表达模式,我们从 "肌肉、活动能力和衰老研究"(Study of Muscle, Mobility, and Aging)的 575 名参与者(年龄≥70 岁)的骨骼肌活检组织中提取了 RNA 并进行了测序。分析了与氧化应激反应相关的 21 种蛋白编码 RNA 的表达水平与六种表型指标的关系,包括肌肉活检组织中线粒体的最大呼吸量(Max OXPHOS)、体能(VO2 峰值、400 米步行速度和腿部力量)和肌肉大小(大腿肌肉体积和全身 D3Cr 肌肉质量)。氧化应激反应基因的 mRNA 水平与各种结果的关系最为一致,这些基因主要在线粒体内表达。编码线粒体蛋白 SOD2、TRX2、PRX3、PRX5 和 GRX2 的 mRNA 表达量较高与线粒体呼吸水平和 VO2 峰值较高有关。此外,更高的 SOD2、PRX3 和 GRX2 表达量与更高的体能表现和肌肉尺寸有关。确定与多种表现和体能领域的高功能相关的特定机制,可能会导致有针对性的抗氧化干预措施,从而对行动能力和独立性产生更大的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Expression of mitochondrial oxidative stress response genes in muscle is associated with mitochondrial respiration, physical performance, and muscle mass in the Study of Muscle, Mobility, and Aging

Expression of mitochondrial oxidative stress response genes in muscle is associated with mitochondrial respiration, physical performance, and muscle mass in the Study of Muscle, Mobility, and Aging

Expression of mitochondrial oxidative stress response genes in muscle is associated with mitochondrial respiration, physical performance, and muscle mass in the Study of Muscle, Mobility, and Aging

Gene expression in skeletal muscle of older individuals may reflect compensatory adaptations in response to oxidative damage that preserve tissue integrity and maintain function. Identifying associations between oxidative stress response gene expression patterns and mitochondrial function, physical performance, and muscle mass in older individuals would further our knowledge of mechanisms related to managing molecular damage that may be targeted to preserve physical resilience. To characterize expression patterns of genes responsible for the oxidative stress response, RNA was extracted and sequenced from skeletal muscle biopsies collected from 575 participants (≥70 years old) from the Study of Muscle, Mobility, and Aging. Expression levels of 21 protein-coding RNAs related to the oxidative stress response were analyzed in relation to six phenotypic measures, including maximal mitochondrial respiration from muscle biopsies (Max OXPHOS), physical performance (VO2 peak, 400-m walking speed, and leg strength), and muscle size (thigh muscle volume and whole-body D3Cr muscle mass). The mRNA level of the oxidative stress response genes most consistently associated across outcomes are preferentially expressed within the mitochondria. Higher expression of mRNAs that encode generally mitochondria located proteins SOD2, TRX2, PRX3, PRX5, and GRX2 were associated with higher levels of mitochondrial respiration and VO2 peak. In addition, greater SOD2, PRX3, and GRX2 expression was associated with higher physical performance and muscle size. Identifying specific mechanisms associated with high functioning across multiple performance and physical domains may lead to targeted antioxidant interventions with greater impacts on mobility and independence.

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来源期刊
Aging Cell
Aging Cell 生物-老年医学
CiteScore
14.40
自引率
2.60%
发文量
212
审稿时长
8 weeks
期刊介绍: Aging Cell, an Open Access journal, delves into fundamental aspects of aging biology. It comprehensively explores geroscience, emphasizing research on the mechanisms underlying the aging process and the connections between aging and age-related diseases.
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