{"title":"牙周炎与牙龈卟啉单胞菌关系的数字化定量分析","authors":"Dong Li, Weiwen Li","doi":"10.1166/jbn.2024.3853","DOIUrl":null,"url":null,"abstract":"Porphyromonas gingivalis (P. gingivalis) constitutes an essential part of the subgingival dental plaque biofilm, serving as a significant factor in the development of periodontitis. Therefore, establishing a rapid and highly sensitive detection method for P. gingivalis\n is crucial to effectively manage periodontitis and its associated complications. In this study, droplet digital PCR (ddPCR) technology was employed for the detection of P. gingivalis, with a detection limit of 101 CFU/mL, exhibiting a 10-fold higher sensitivity compared to\n qPCR (with a sensitivity of 102 CFU/mL). Furthermore, no cross-reactivity was observed with four other bacterial species. In comparison to real-time quantitative PCR, ddPCR demonstrated enhanced sensitivity in detecting P. gingivalis at lower concentrations in 16 simulated\n samples, indicating its applicability for rapid detection of P. gingivalis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Digital Quantitative Analysis of the Relationship Between Periodontitis and Porphyromonas gingivalis\",\"authors\":\"Dong Li, Weiwen Li\",\"doi\":\"10.1166/jbn.2024.3853\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Porphyromonas gingivalis (P. gingivalis) constitutes an essential part of the subgingival dental plaque biofilm, serving as a significant factor in the development of periodontitis. Therefore, establishing a rapid and highly sensitive detection method for P. gingivalis\\n is crucial to effectively manage periodontitis and its associated complications. In this study, droplet digital PCR (ddPCR) technology was employed for the detection of P. gingivalis, with a detection limit of 101 CFU/mL, exhibiting a 10-fold higher sensitivity compared to\\n qPCR (with a sensitivity of 102 CFU/mL). Furthermore, no cross-reactivity was observed with four other bacterial species. In comparison to real-time quantitative PCR, ddPCR demonstrated enhanced sensitivity in detecting P. gingivalis at lower concentrations in 16 simulated\\n samples, indicating its applicability for rapid detection of P. gingivalis.\",\"PeriodicalId\":15260,\"journal\":{\"name\":\"Journal of biomedical nanotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomedical nanotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1166/jbn.2024.3853\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2024.3853","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Digital Quantitative Analysis of the Relationship Between Periodontitis and Porphyromonas gingivalis
Porphyromonas gingivalis (P. gingivalis) constitutes an essential part of the subgingival dental plaque biofilm, serving as a significant factor in the development of periodontitis. Therefore, establishing a rapid and highly sensitive detection method for P. gingivalis
is crucial to effectively manage periodontitis and its associated complications. In this study, droplet digital PCR (ddPCR) technology was employed for the detection of P. gingivalis, with a detection limit of 101 CFU/mL, exhibiting a 10-fold higher sensitivity compared to
qPCR (with a sensitivity of 102 CFU/mL). Furthermore, no cross-reactivity was observed with four other bacterial species. In comparison to real-time quantitative PCR, ddPCR demonstrated enhanced sensitivity in detecting P. gingivalis at lower concentrations in 16 simulated
samples, indicating its applicability for rapid detection of P. gingivalis.