对从鼻窦炎患者中分离出的五种 Dipodascus Capitus 核糖体扩增子进行测序

Iman Hadi, Ahmed Abdel-Azeem
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引用次数: 0

摘要

测序反应显示了所调查样本的准确身份,并揭示所有调查样本都与岬蛙 Dipodascus capitatus(GenBank 编号:OW983902.1)同源。与 D. capitatus 序列的参考序列相比,发现了一个核酸变体。在 S5 样品中检测到的核酸替换(359A>T)代表了该变异。与此同时,其他样本与相应的序列显示出完全的同源性,与蝙蝠的参考序列相比没有显示出任何可检测到的核酸变异。从树状图中可以推断,与其他野生型序列相比,检测到的核酸置换显示出观察到的变异对被调查的 S5 样本进化定位的改变有轻微影响。这是由于与野生型样本相比,变异样本在其他位置的定位发生了变化。从该树中可以推断出,我们所研究的蝙蝠蛾样本与来自不同国家的各种菌株相邻。此外,这棵树中的邻近系统发育距离表明绒毛膜促性腺激素序列具有独特的生物多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sequencing of ribosome-based amplicons in five isolates Dipodascus Capitus from patients with sinusitis
Sequencing reactions showed the accurate identity of the investigated samples and revealed that all investigated samples were homologous to Dipodascus capitatus (GenBank acc. no. OW983902.1). The presence of one nucleic acid variant compared with the referring sequences of the D. capitatussequences was demonstrated. The identified variant was represented by a nucleic acid substitution (359A>T) detected in the S5 sample. Meanwhile, the rest of the samples showed a complete homology with the corresponding sequences and did not exhibit any detectable nucleic acid variations in comparison with the D. capitatus reference sequences. It was inferred from the tree that the detected nucleic acid substitutions showed a slight effect of the observed variations on the altering evolutionary positioning of the investigated S5 sample in comparison with the other wild-type sequences. This was due to the positioning of the altered samples in the other positions compared with the wild-type counterparts. It was inferred from the tree that our investigated D. capitatus samples were suited in the immediate vicinity to various strains deposited from various multi-national positions. Furthermore, neighbor phylogenetic distances in this tree indicated a distinct biological diversity of D. capitatus sequences.
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