洞察从巴西不同来源分离的弗雷德里克森耶尔森菌、中间耶尔森菌和克里斯滕森耶尔森菌的基因组特征。

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Felipe Pinheiro Vilela, Priscilla Fernanda Martins Imori, Marc William Allard, Juliana Pfrimer Falcão
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引用次数: 0

摘要

耶尔森菌是一个重要的菌属,包括食源性细菌、人畜共患病细菌和致病细菌。另一方面,尽管有一些关于人类感染的报道,但对所谓的类耶尔森菌(Yersinia enterocolitica-like)的物种研究不足,而且大多被定性为非致病菌。本研究旨在对全球分离到的弗氏耶尔森菌(YF)、中间耶尔森菌(YI)和克里斯滕森耶尔森菌(YK)进行基因组研究。共对 22 个 YF、20 个 YI 和 14 个 YK 基因组进行了抗菌性基因、质粒、噬菌体和毒力因子的检索。通过 Gegenees 和核心基因组多焦点序列分型分析了它们的系统发生学相关性。在不到五个基因组中检测到β-内酰胺抗性基因 blaTEM-116 和五个质粒复制子(pYE854、ColRNAI、ColE10、Col(pHAD28) 和 IncN3)。共检测到 59 个噬菌体、106 个耶尔森菌属毒力标记(与粘附、抗吞噬、外酵素、侵袭、铁吸收、蛋白酶、分泌系统和 O 抗原有关)以及与其他 20 个细菌属有关的毒力因子。系统发生组分析表明,种间差异很大,YF 有四个高度多样化的群集。总之,通过分析 YF、YI 和 YK 基因组获得的结果表明,这些菌株具有广泛的多样性和高频率的噬菌体和毒力因子,因而具有潜在的毒力。系统发育分析能够正确区分这些密切相关的物种,并显示出不同基因亚群的存在。这些数据有助于更好地了解 YF、YI 和 YK 的毒力相关特征和全球遗传多样性,并加强了更好地描述这些被认为是非致病性的小肠结肠炎酵母菌类的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Insights into the genomic traits of Yersinia frederiksenii, Yersinia intermedia and Yersinia kristensenii isolated from diverse sources in Brazil

Insights into the genomic traits of Yersinia frederiksenii, Yersinia intermedia and Yersinia kristensenii isolated from diverse sources in Brazil

Insights into the genomic traits of Yersinia frederiksenii, Yersinia intermedia and Yersinia kristensenii isolated from diverse sources in Brazil

Yersinia is an important genus comprising foodborne, zoonotic and pathogenic bacteria. On the other hand, species of the so-called group Yersinia enterocolitica-like are understudied and mostly characterized as non-pathogenic, despite of some reports of human infections. The present study aimed to provide genomic insights of Yersinia frederiksenii (YF), Yersinia intermedia (YI) and Yersinia kristensenii (YK) isolated worldwide. A total of 22 YF, 20 YI and 14 YK genomes were searched for antimicrobial resistance genes, plasmids, prophages, and virulence factors. Their phylogenomic relatedness was analyzed by Gegenees and core-genome multi-locus sequence typing. Beta-lactam resistance gene blaTEM-116 and five plasmids replicons (pYE854, ColRNAI, ColE10, Col(pHAD28) and IncN3) were detected in less than five genomes. A total of 59 prophages, 106 virulence markers of the Yersinia genus, associated to adherence, antiphagocytosis, exoenzymes, invasion, iron uptake, proteases, secretion systems and the O-antigen, and virulence factors associated to other 20 bacterial genera were detected. Phylogenomic analysis revealed high inter-species distinction and four highly diverse YF clusters. In conclusion, the results obtained through the analyses of YF, YI and YK genomes suggest the virulence potential of these strains due to the broad diversity and high frequency of prophages and virulence factors found. Phylogenetic analyses were able to correctly distinguish these closely related species and show the presence of different genetic subgroups. These data contributed for a better understanding of YF, YI and YK virulence-associated features and global genetic diversity, and reinforced the need for better characterization of these Y. enterocolitica-like species considered non-pathogenic.

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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