Hua Shi, Xiaojian Zhang, Pan Ge, Victoria Meliopoulos, Pam Freiden, Brandi Livingston, Stacey Schultz-Cherry, Ted M Ross
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引用次数: 0
摘要
流感病毒导致季节性呼吸道疾病,每年影响全球数百万人。预防性疫苗是防止流感流行的推荐方法。目前的一种商用流感疫苗由灭活病毒组成,在新流感季节开始前几个月进行挑选。在许多季节中,这些疫苗的有效性(VE)可能相对较低。因此,迫切需要开发一种改进的、更通用的流感疫苗 (UIV),为所有年龄组的人提供广泛的保护,以抵御各种漂移毒株。为了满足这一需求,我们开发了计算优化广泛反应抗原(COBRA)方法来设计一种血凝素(HA)分子作为新型流感疫苗。本研究开发了基于 COBRA HA 的灭活流感病毒 (IIV),该病毒表达了来自 H1 或 H3 流感病毒的 COBRA HA,并在免疫幼稚动物模型或流感免疫前动物模型中激发了即刻和长期的保护性免疫。这些结果与接种了表达野生型 H1 或 H3 HA 蛋白(WT-IIV)的 IIV 疫苗的动物进行了比较。COBRA-IIV 能激发持久的广泛反应性抗体,对流感病毒的变种具有血凝抑制(HAI)活性。
The influenza viruses cause seasonal respiratory illness that affect millions of people globally every year. Prophylactic vaccines are the recommended method to prevent the breakout of influenza epidemics. One of the current commercial influenza vaccines consists of inactivated viruses that are selected months prior to the start of a new influenza season. In many seasons, the vaccine effectiveness (VE) of these vaccines can be relatively low. Therefore, there is an urgent need to develop an improved, more universal influenza vaccine (UIV) that can provide broad protection against various drifted strains in all age groups. To meet this need, the computationally optimized broadly reactive antigen (COBRA) methodology was developed to design a hemagglutinin (HA) molecule as a new influenza vaccine. In this study, COBRA HA-based inactivated influenza viruses (IIV) expressing the COBRA HA from H1 or H3 influenza viruses were developed and characterized for the elicitation of immediate and long-term protective immunity in both immunologically naïve or influenza pre-immune animal models. These results were compared to animals vaccinated with IIV vaccines expressing wild-type H1 or H3 HA proteins (WT-IIV). The COBRA-IIV elicited long-lasting broadly reactive antibodies that had hemagglutination-inhibition (HAI) activity against drifted influenza variants.
期刊介绍:
(formerly Human Vaccines; issn 1554-8619)
Vaccine research and development is extending its reach beyond the prevention of bacterial or viral diseases. There are experimental vaccines for immunotherapeutic purposes and for applications outside of infectious diseases, in diverse fields such as cancer, autoimmunity, allergy, Alzheimer’s and addiction. Many of these vaccines and immunotherapeutics should become available in the next two decades, with consequent benefit for human health. Continued advancement in this field will benefit from a forum that can (A) help to promote interest by keeping investigators updated, and (B) enable an exchange of ideas regarding the latest progress in the many topics pertaining to vaccines and immunotherapeutics.
Human Vaccines & Immunotherapeutics provides such a forum. It is published monthly in a format that is accessible to a wide international audience in the academic, industrial and public sectors.