[DARS2在结直肠癌中的表达及其临床意义]。

Q3 Medicine
L Ma, H L Yang, S H Huang, L L Huang, C L Wang, J H Mei
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引用次数: 0

摘要

目的:研究 DARS2 在结直肠癌中的表达及其临床意义:研究 DARS2 在结直肠癌中的表达及其临床意义。方法本研究使用生物信息学工具,特别是基因表达谱交互分析 2(GEPIA2),对 DARS2 在结直肠癌组织中的表达进行了深入分析。研究人员对南昌大学第一附属医院的 108 例结直肠癌标本和 30 例正常结直肠组织进行了免疫组化染色。用小干扰 RNA(siRNA)和 DARS2 过表达质粒转染结直肠癌细胞株(HCT116 和 SW480),以检测 DARS2 基因敲除和过表达对细胞功能的影响。为了评估对细胞功能的影响,分别使用了 CCK8 和经孔迁移试验来评估细胞增殖和细胞运动。此外,还采用蛋白免疫印迹法仔细检查了结直肠癌细胞上皮-间质转化相关蛋白的表达。结果显示与正常上皮细胞相比,DARS2在结直肠癌组织中的表达明显上调。此外,DARS2 在Ⅲ-Ⅳ期结直肠癌中的表达高于Ⅰ-Ⅱ期结直肠癌,与 N 分期、M 分期和病理分期(PPPPP 结论:DARS2 在结直肠癌组织中的表达与 N 分期、M 分期和病理分期(PPPPP 结论:DARS2 在结直肠癌组织中的表达与 N 分期、M 分期和病理分期(PPPPP 结论:DARS2 在结直肠癌组织中的表达与 N 分期、M 分期和病理分期(PPPPPDARS2的表达与结直肠癌的进展密切相关。沉默 DARS2 可抑制细胞增殖和迁移,对上皮-间质转化过程产生明显影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Expression of DARS2 in colorectal cancer and its clinical significance].

Objective: To investigate the expression of DARS2 and its clinical significance in colorectal cancer. Methods: In this study, bioinformatics tools, especially gene expression profile interactive analysis 2 (GEPIA2), were used to conduct an in-depth analysis of DARS2 expression in colorectal cancer tissues. Immunohistochemical staining was carried out in 108 colorectal cancer specimens and 30 normal colorectal tissues obtained from the First Affiliated Hospital of Nanchang University, Nanchang, China. Colorectal cancer cell lines (HCT116 and SW480) were transfected with small interfering RNA (siRNA) and DARS2 overexpression plasmid to examine the effects of DARS2 knockdown and overexpression on cell function. To assess the effects on cell function, CCK8 and transwell migration assays were used to assess proliferation and cell motility, respectively. Additionally, protein immunoblotting was employed to scrutinize the expression of proteins associated with the epithelial-mesenchymal transition of colorectal cancer cells. Results: DARS2 exhibited a pronounced upregulation in expression within colorectal cancer tissues compared to their normal epithelial counterparts. Furthermore, DARS2 expression was higher in colorectal cancer of stage Ⅲ-Ⅳ than those of stage Ⅰ-Ⅱ, exhibiting a significant correlation with N staging, M staging, and pathological staging (P<0.05). Kaplan-Meier analyses showed a decreased overall survival rate in colorectal cancer with DARS2 expression compared to those without DARS2 expression (P<0.05). In the siRNA transfection group, there was a significant reduction in cell proliferation and migration (P<0.01 and P<0.05, respectively). Conversely, the transfection of DARS2 overexpression plasmids substantially increased both cell proliferation and migration (P<0.05). Additionally, immunoblotting revealed that DARS2 knockdown led to an upregulation of E-cadherin expression and a downregulation of N-cadherin and vimentin expression. In contrast, DARS2 overexpression resulted in increased N-cadherin and vimentin expression, coupled with reduction in E-cadherin expression. Conclusions: There is a strong association between DARS2 expression and colorectal cancer progression. Silencing DARS2 inhibits cell proliferation and migration, exerting a discernible influence on the epithelial-mesenchymal transition process.

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中华病理学杂志
中华病理学杂志 Medicine-Medicine (all)
CiteScore
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10377
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