N M Bekedam, E L Koot, E M V de Cuba, M J A van Alphen, R L P van Veen, L H E Karssemakers, L E Smeele, M B Karakullukcu
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After standardized histopathological processing, all hematoxylin-eosin whole slide images (WSI) were included for analysis. Corresponding US images were found based on the known WSI spacing and fiducials. Blinded observers measured the tumor thickness and the margin in the caudal, deep, and cranial directions on every slide. The anterior and posterior margin was measured per specimen.</p><p><strong>Results: </strong>The mean difference in all measurements between manually segmented US and histopathology was 2.34 (SD: ±3.34) mm, and Spearman's rank correlation coefficient was 0.733 (p < 0.001). The smallest mean difference was in the tumor thickness with 0.80 (SD: ±2.44) mm and a correlation of 0.836 (p < 0.001). Limitations were observed in the caudal region, where no correlation was found.</p><p><strong>Conclusion: </strong>This study shows that 3D US and histopathology have a moderate to strong statistically significant correlation (r = 0.733; p < 0.001) and a mean difference between the modalities of 2.3 mm (95%CI: -4.2; 8.9). Future research should focus on patient outcomes regarding resection margins.</p>","PeriodicalId":11952,"journal":{"name":"European Archives of Oto-Rhino-Laryngology","volume":" ","pages":"5455-5463"},"PeriodicalIF":1.9000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11416359/pdf/","citationCount":"0","resultStr":"{\"title\":\"Clinical validation of the accuracy of an intra-operative assessment tool using 3D ultrasound compared to histopathology in patients with squamous cell carcinoma of the tongue.\",\"authors\":\"N M Bekedam, E L Koot, E M V de Cuba, M J A van Alphen, R L P van Veen, L H E Karssemakers, L E Smeele, M B Karakullukcu\",\"doi\":\"10.1007/s00405-024-08753-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Histopathological analysis often shows close resection margins after surgical removal of tongue squamous cell carcinoma (TSCC). 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引用次数: 0
摘要
背景:组织病理学分析显示,舌鳞状细胞癌(TSCC)手术切除后的切除边缘通常很近。本研究旨在探讨术中三维超声(US)边缘评估与切除的 TSCC 术后组织病理学之间的一致性:方法:本研究前瞻性地纳入了 10 例患者。在标本中插入三个靶标插管。为了获取电动三维 US 体积,切除的标本浸没在生理盐水中,然后在探针在标本上移动的同时获取图像。对 US 图像进行两次标注:(1) 自动标注;(2) 手动标注,并将自动分割作为初始化。经过标准化组织病理学处理后,所有苏木精-伊红全玻片图像(WSI)都被纳入分析范围。根据已知的 WSI 间距和靶标找到相应的 US 图像。双盲观察员测量每张切片上肿瘤的厚度以及尾部、深部和头颅方向的边缘。每个标本的前后边缘都进行了测量:结果:手动分割的 US 和组织病理学所有测量值的平均值相差 2.34(SD:±3.34)毫米,斯皮尔曼等级相关系数为 0.733(P 结论:三维 US 和组织病理学测量值的平均值相差 2.34(SD:±3.34)毫米,斯皮尔曼等级相关系数为 0.733:这项研究表明,三维 US 和组织病理学在统计学上具有中度到高度的相关性(r = 0.733; p
Clinical validation of the accuracy of an intra-operative assessment tool using 3D ultrasound compared to histopathology in patients with squamous cell carcinoma of the tongue.
Background: Histopathological analysis often shows close resection margins after surgical removal of tongue squamous cell carcinoma (TSCC). This study aimed to investigate the agreement between intraoperative 3D ultrasound (US) margin assessment and postoperative histopathology of resected TSCC.
Methods: In this study, ten patients were prospectively included. Three fiducial cannulas were inserted into the specimen. To acquire a motorized 3D US volume, the resected specimen was submerged in saline, after which images were acquired while the probe moved over the specimen. The US volumes were annotated twice: (1) automatically and (2) manually, with the automatic segmentation as initialization. After standardized histopathological processing, all hematoxylin-eosin whole slide images (WSI) were included for analysis. Corresponding US images were found based on the known WSI spacing and fiducials. Blinded observers measured the tumor thickness and the margin in the caudal, deep, and cranial directions on every slide. The anterior and posterior margin was measured per specimen.
Results: The mean difference in all measurements between manually segmented US and histopathology was 2.34 (SD: ±3.34) mm, and Spearman's rank correlation coefficient was 0.733 (p < 0.001). The smallest mean difference was in the tumor thickness with 0.80 (SD: ±2.44) mm and a correlation of 0.836 (p < 0.001). Limitations were observed in the caudal region, where no correlation was found.
Conclusion: This study shows that 3D US and histopathology have a moderate to strong statistically significant correlation (r = 0.733; p < 0.001) and a mean difference between the modalities of 2.3 mm (95%CI: -4.2; 8.9). Future research should focus on patient outcomes regarding resection margins.
期刊介绍:
Official Journal of
European Union of Medical Specialists – ORL Section and Board
Official Journal of Confederation of European Oto-Rhino-Laryngology Head and Neck Surgery
"European Archives of Oto-Rhino-Laryngology" publishes original clinical reports and clinically relevant experimental studies, as well as short communications presenting new results of special interest. With peer review by a respected international editorial board and prompt English-language publication, the journal provides rapid dissemination of information by authors from around the world. This particular feature makes it the journal of choice for readers who want to be informed about the continuing state of the art concerning basic sciences and the diagnosis and management of diseases of the head and neck on an international level.
European Archives of Oto-Rhino-Laryngology was founded in 1864 as "Archiv für Ohrenheilkunde" by A. von Tröltsch, A. Politzer and H. Schwartze.