Ca2+ 传感器 GbCML45 和 GbCML50 在提高棉花轮纹病抗性中的积极作用。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Feifei Yi, Yuzhe Li, Aosong Song, Xinying Shi, Shanci Hu, Shuang Wu, Lili Shao, Zongyan Chu, Kun Xu, Liangliang Li, Lam-Son Phan Tran, Weiqiang Li, Yingfan Cai
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引用次数: 0

摘要

作为一种通用的第二信使,细胞膜钙(Ca2+)在细胞内的多方面过程中发挥作用,包括植物的生长、发育和对生物/非生物胁迫的反应。植物特有的 Ca2+ 传感器、钙调素和类钙调素(CML)蛋白作为第二信使系统的成员,将 Ca2+ 信号转导到下游反应中。然而,CMLs 在棉花(Gossypium spp.)感染大丽轮枝菌(Verticillium dahliae)(大丽轮枝菌会引起严重的维管束病害)后的反应中的功能仍然难以捉摸。在这里,我们发现大丽轮枝菌感染棉花后,GbCML45 在棉花根部的表达水平会升高,这表明它在抗轮枝菌枯萎病中的潜在作用。我们发现,在棉花植株中敲除 GbCML45 会降低其抗性,而在拟南芥植株中过表达 GbCML45 则会增强其对大丽花病毒感染的抗性。此外,通过酵母双杂交和双分子荧光测定,GbCML45 与其近源同源物 GbCML50 之间存在生理相互作用,而且在基因敲除研究中,这两种蛋白都以 Ca2+ 依赖性方式增强了棉花对大丽花病毒感染的抗性。详细研究表明,几种防御相关途径,包括水杨酸、乙烯、活性氧和一氧化氮信号途径,以及木质素和胼胝质的积累,都是 GbCML45 和 GbCML50 调节棉花抗大丽花病毒性的原因。这些结果共同表明,GbCML45 和 GbCML50 是提高棉花轮纹枯萎病抗性的积极调节因子,为通过基因工程和分子育种改良耐轮纹枯萎病棉花品种提供了潜在的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Positive roles of the Ca2+ sensors GbCML45 and GbCML50 in improving cotton Verticillium wilt resistance.

As a universal second messenger, cytosolic calcium (Ca2+) functions in multifaceted intracellular processes, including growth, development and responses to biotic/abiotic stresses in plant. The plant-specific Ca2+ sensors, calmodulin and calmodulin-like (CML) proteins, function as members of the second-messenger system to transfer Ca2+ signal into downstream responses. However, the functions of CMLs in the responses of cotton (Gossypium spp.) after Verticillium dahliae infection, which causes the serious vascular disease Verticillium wilt, remain elusive. Here, we discovered that the expression level of GbCML45 was promoted after V. dahliae infection in roots of cotton, suggesting its potential role in Verticillium wilt resistance. We found that knockdown of GbCML45 in cotton plants decreased resistance while overexpression of GbCML45 in Arabidopsis thaliana plants enhanced resistance to V. dahliae infection. Furthermore, there was physiological interaction between GbCML45 and its close homologue GbCML50 by using yeast two-hybrid and bimolecular fluorescence assays, and both proteins enhanced cotton resistance to V. dahliae infection in a Ca2+-dependent way in a knockdown study. Detailed investigations indicated that several defence-related pathways, including salicylic acid, ethylene, reactive oxygen species and nitric oxide signalling pathways, as well as accumulations of lignin and callose, are responsible for GbCML45- and GbCML50-modulated V. dahliae resistance in cotton. These results collectively indicated that GbCML45 and GbCML50 act as positive regulators to improve cotton Verticillium wilt resistance, providing potential targets for exploitation of improved Verticillium wilt-tolerant cotton cultivars by genetic engineering and molecular breeding.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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