在豆科植物拔节过程中,通过磷酸化介导的 RING 型 E3 连接酶的功能转换,对拔节因子受体水平进行动态调节。

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-05-31 DOI:10.1016/j.molp.2024.05.010
Hao Li, Yajuan Ou, Jidan Zhang, Kui Huang, Ping Wu, Xiaoli Guo, Hui Zhu, Yangrong Cao
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引用次数: 0

摘要

受体水平的精确控制对于启动细胞信号传导以响应特定配体至关重要,然而,在感知 NF 以建立共生关系的过程中,调节结核因子(NF)受体(NFR1/NFR5)的这种机制仍不清楚。本研究揭示了NFR-Interacting RING型E3连接酶1(NIRE1)在调节NFR1/NFR5平衡以优化日本莲的根瘤菌感染和结核发育中的关键作用。NIRE1 在这一调控过程中具有双重功能。NIRE1 同时与 NFR1/NFR5 结合,在接种根瘤菌之前通过与 K48 链接的多泛素化促进它们的降解。接种根瘤菌后,NFR1 在一个保守残基 Tyr-109 上磷酸化 NIRE1,从而诱导 NIRE1 的功能转换。这种转换使 NIRE1 能够介导 K63 链接的多泛素化,从而稳定受感染根细胞中的 NFR1/NFR5。引入磷酸化死亡的 NIRE1Y109F 会导致结核发育延迟,这突出了 Tyr-109 处的磷酸化在协调共生过程中的重要性。相反,表达磷酸化模拟 NIRE1Y109E 会导致日本莴苣自发形成结核,这进一步强调了 NIRE1 中磷酸化依赖性功能开关的关键作用。总之,这些发现提供了单个 E3 连接酶进行磷酸化依赖性功能转换、动态和精确调节 NF 受体蛋白水平的首例证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic modulation of nodulation factor receptor levels by phosphorylation-mediated functional switch of a RING-type E3 ligase during legume nodulation.

The precise control of receptor levels is crucial for initiating cellular signaling transduction in response to specific ligands; however, such mechanisms regulating nodulation factor (NF) receptor (NFR)-mediated perception of NFs to establish symbiosis remain unclear. In this study, we unveil the pivotal role of the NFR-interacting RING-type E3 ligase 1 (NIRE1) in regulating NFR1/NFR5 homeostasis to optimize rhizobial infection and nodule development in Lotus japonicus. We demonstrated that NIRE1 has a dual function in this regulatory process. It associates with both NFR1 and NFR5, facilitating their degradation through K48-linked polyubiquitination before rhizobial inoculation. However, following rhizobial inoculation, NFR1 phosphorylates NIRE1 at a conserved residue, Tyr-109, inducing a functional switch in NIRE1, which enables NIRE1 to mediate K63-linked polyubiquitination, thereby stabilizing NFR1/NFR5 in infected root cells. The introduction of phospho-dead NIRE1Y109F leads to delayed nodule development, underscoring the significance of phosphorylation at Tyr-109 in orchestrating symbiotic processes. Conversely, expression of the phospho-mimic NIRE1Y109E results in the formation of spontaneous nodules in L. japonicus, further emphasizing the critical role of the phosphorylation-dependent functional switch in NIRE1. In summary, these findings uncover a fine-tuned symbiotic mechanism that a single E3 ligase could undergo a phosphorylation-dependent functional switch to dynamically and precisely regulate NF receptor protein levels.

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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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