FdeC 的表达调控禽致病性大肠杆菌 IMT5155 菌株的运动性和粘附性。

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Adrianna Aleksandrowicz, Rikke Brødsgaard Kjærup, Krzysztof Grzymajło, Fernando Garcia Martinez, Javier Muñoz, Dominika Borowska, Samantha Sives, Lonneke Vervelde, Tina Sørensen Dalgaard, Robert A Kingsley, Rafał Kolenda
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引用次数: 0

摘要

禽致病性大肠杆菌(APEC)对宿主环境(包括毒力因子表达)变化的适应对疾病的发展至关重要。FdeC 是一种自转运粘附素,在尿路致病性大肠杆菌(UPEC)粘附上皮细胞的过程中发挥作用。已知 fdeC 的表达受 UPEC 和产志贺毒素大肠杆菌(STEC)环境条件的调节。之前的一项研究发现,转座子插入破坏了 fdeC 基因的 APEC 菌株 IMT5155 会增强对鸡肠道细胞的粘附力,这促使我们进一步探索 fdeC 在感染中的作用。我们发现,在 APEC 和非致病性大肠杆菌基因组中,fdeC 基因的流行率和 FdeC 变异流行率有所不同。fdeC 基因的表达在宿主体温(一种与感染相关的条件)下被诱导。与野生型(WT)相比,fdeC 基因的破坏导致 CHIC-8E11 细胞粘附力增强,42 °C时运动能力增强,多种转运蛋白的表达量增加,从而增加了无机离子的输出。运动性增加可能与无机离子输出增加有关,因为这导致了 YbjN(一种已知抑制运动性的蛋白质)的下调。在实验感染中,APEC 菌株 IMT5155 中的 fdeC 失活导致鸡的免疫反应弱于 WT。我们的研究结果表明,FdeC 在宿主体内上调,并在定殖过程中通过下调运动性来促进与宿主的相互作用。深入了解 FdeC 的调控和功能可为大肠杆菌的致病机理提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155.

Adaptation of avian pathogenic E. coli (APEC) to changing host environments including virulence factors expression is vital for disease progression. FdeC is an autotransporter adhesin that plays a role in uropathogenic Escherichia coli (UPEC) adhesion to epithelial cells. Expression of fdeC is known to be regulated by environmental conditions in UPEC and Shiga toxin-producing E. coli (STEC). The observation in a previous study that an APEC strain IMT5155 in which the fdeC gene was disrupted by a transposon insertion resulted in elevated adhesion to chicken intestinal cells prompted us to further explore the role of fdeC in infection. We found that the fdeC gene prevalence and FdeC variant prevalence differed between APEC and nonpathogenic E. coli genomes. Expression of the fdeC gene was induced at host body temperature, an infection relevant condition. Disruption of fdeC resulted in greater adhesion to CHIC-8E11 cells and increased motility at 42 °C compared to wild type (WT) and higher expression of multiple transporter proteins that increased inorganic ion export. Increased motility may be related to increased inorganic ion export since this resulted in downregulation of YbjN, a protein known to supress motility. Inactivation of fdeC in APEC strain IMT5155 resulted in a weaker immune response in chickens compared to WT in experimental infections. Our findings suggest that FdeC is upregulated in the host and contributes to interactions with the host by down-modulating motility during colonization. A thorough understanding of the regulation and function of FdeC could provide novel insights into E. coli pathogenesis.

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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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