揭示植物特殊代谢途径的化学工具

IF 8.3 2区 生物学 Q1 PLANT SCIENCES
Benedikt Seligmann , Shenyu Liu , Jakob Franke
{"title":"揭示植物特殊代谢途径的化学工具","authors":"Benedikt Seligmann ,&nbsp;Shenyu Liu ,&nbsp;Jakob Franke","doi":"10.1016/j.pbi.2024.102554","DOIUrl":null,"url":null,"abstract":"<div><p>Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate the discovery of biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry and chemical biology helped to overcome key challenges of pathway elucidation. Based on recent examples, we describe how state-of-the-art isotope labelling experiments can guide the selection of biosynthetic gene candidates, how affinity-based probes enable the identification of novel enzymes, how semisynthesis can improve the availability of elusive pathway intermediates, and how biomimetic reactions provide a better understanding of inherent chemical reactivity. We anticipate that a wider application of such chemical methods will accelerate the pace of pathway elucidation in plants.</p></div>","PeriodicalId":11003,"journal":{"name":"Current opinion in plant biology","volume":"80 ","pages":"Article 102554"},"PeriodicalIF":8.3000,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1369526624000451/pdfft?md5=0d15c185e80b62b4200a38c84bce7bf0&pid=1-s2.0-S1369526624000451-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Chemical tools for unpicking plant specialised metabolic pathways\",\"authors\":\"Benedikt Seligmann ,&nbsp;Shenyu Liu ,&nbsp;Jakob Franke\",\"doi\":\"10.1016/j.pbi.2024.102554\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate the discovery of biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry and chemical biology helped to overcome key challenges of pathway elucidation. Based on recent examples, we describe how state-of-the-art isotope labelling experiments can guide the selection of biosynthetic gene candidates, how affinity-based probes enable the identification of novel enzymes, how semisynthesis can improve the availability of elusive pathway intermediates, and how biomimetic reactions provide a better understanding of inherent chemical reactivity. We anticipate that a wider application of such chemical methods will accelerate the pace of pathway elucidation in plants.</p></div>\",\"PeriodicalId\":11003,\"journal\":{\"name\":\"Current opinion in plant biology\",\"volume\":\"80 \",\"pages\":\"Article 102554\"},\"PeriodicalIF\":8.3000,\"publicationDate\":\"2024-05-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1369526624000451/pdfft?md5=0d15c185e80b62b4200a38c84bce7bf0&pid=1-s2.0-S1369526624000451-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current opinion in plant biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1369526624000451\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current opinion in plant biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369526624000451","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

阐明植物中特殊代谢物的生化途径是实现或改进其可持续生物技术生产的关键。化学工具可以极大地促进生物合成基因和酶的发现。在此,我们总结了跨学科方法,其中化学和化学生物学的方法有助于克服途径阐明的关键挑战。根据最近的实例,我们介绍了最先进的同位素标记实验如何指导候选生物合成基因的选择,基于亲和力的探针如何使新型酶的鉴定成为可能,半合成如何提高难以捉摸的途径中间体的可用性,以及仿生反应如何使人们更好地了解固有的化学反应性。我们预计,此类化学方法的更广泛应用将加快植物途径阐明的步伐。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemical tools for unpicking plant specialised metabolic pathways

Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate the discovery of biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry and chemical biology helped to overcome key challenges of pathway elucidation. Based on recent examples, we describe how state-of-the-art isotope labelling experiments can guide the selection of biosynthetic gene candidates, how affinity-based probes enable the identification of novel enzymes, how semisynthesis can improve the availability of elusive pathway intermediates, and how biomimetic reactions provide a better understanding of inherent chemical reactivity. We anticipate that a wider application of such chemical methods will accelerate the pace of pathway elucidation in plants.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Current opinion in plant biology
Current opinion in plant biology 生物-植物科学
CiteScore
16.30
自引率
3.20%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Current Opinion in Plant Biology builds on Elsevier's reputation for excellence in scientific publishing and long-standing commitment to communicating high quality reproducible research. It is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion legacy - of editorial excellence, high-impact, and global reach - to ensure they are a widely read resource that is integral to scientists' workflow.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信