Saima Iqbal , Sabia Qureshi , Muddasir S. Banday , Shaheen Farooq , Zahid A. Kashoo , Maliha Gulzar , M. Altaf Bhat , Arif Pandit , Md. Isfaqul Hussain , Pervaiz Dar , Gulzar Badroo , Mahrukh Hafiz , Faheem ud Din , Junaid Mehraj
{"title":"印度家禽和禽肉中短可变区 flaA 基因(SVR-flaA)的多样性和耐多药弯曲杆菌的毒力特征。","authors":"Saima Iqbal , Sabia Qureshi , Muddasir S. Banday , Shaheen Farooq , Zahid A. Kashoo , Maliha Gulzar , M. Altaf Bhat , Arif Pandit , Md. Isfaqul Hussain , Pervaiz Dar , Gulzar Badroo , Mahrukh Hafiz , Faheem ud Din , Junaid Mehraj","doi":"10.1016/j.jfp.2024.100308","DOIUrl":null,"url":null,"abstract":"<div><p>Human gastrointestinal infections caused by <em>Campylobacter</em> species is the second most important foodborne illness after salmonellosis worldwide. Poultry represent one of the main sources of <em>Campylobacter</em> organisms. In the present study, the short variable region of flagellin gene (SVR-<em>fla</em>A) typing was carried out to determine the variation among the circulating strains of <em>Campylobacter jejuni</em> and <em>Campylobacter coli</em>. The <em>C. jejuni</em> and <em>C. coli</em> isolated from poultry and poultry meat were screened for the presence of virulence determinants like <em>cad</em>F, <em>fla</em>A, <em>cdt</em>B, and <em>wla</em>N gene. The screening for <em>wla</em>N gene is crucial in view of the fact that most patients with Guillian Barre’s (GB) syndrome with a preceding history of diarrheal illness have been found to harbor <em>wla</em>N gene−positive <em>C jejuni</em> strains. Out of the 200 samples comprising poultry meat and cloacal swabs, 21.5% of samples were found to harbor <em>Campylobacter</em> spp. of which 2.5% were <em>Campylobacter jejuni</em>, and 19% were confirmed as <em>Campylobacter coli</em>. The <em>cad</em>F, <em>fla</em>A, <em>cdt</em>B virulence genes were detected in all the <em>Campylobacter</em> spp. isolated in the present study. The presence of the <em>wla</em>N gene in the <em>Campylobacter jejuni</em> isolated in the present study may pose a public health threat with long−term human health implications. The SVR-<em>fla</em>A typing of twelve <em>Campylobacter</em> isolates obtained in the present study revealed that <em>Campylobacter coli fla</em>A sequence OL471375 is a new strain with a novel allele type 1,675 and peptide sequence 5 which stands deposited in pubMLST database for <em>Campylobacter</em>. The other <em>fla</em>A-SVR gene sequences identified in this study were OL471369, OL471370, OL471371, OL471372, OL471373, and OL471374. Among twelve <em>Campylobacter</em> spp., three distinct <em>Dde</em>I-RFLP patterns were observed, each varying in size from 100 to 1,000 base pairs. Antimicrobial profiling of the <em>Campylobacter</em> spp. isolated in the present study revealed that 50% of the strains were multidrug resistant. All the <em>Campylobacter</em> spp. were resistant to ciprofloxacin (CIP), ampicillin (AMP), penicillin (PEN), and nalidixic acid (NAL) whereas 57.1% of strains were resistant to tetracycline (TET) and erythromycin (ERY) 28% to amoxicillin (AMX) and enrofloxacin (ENO), 85% to amikacin (AMK). The high degree of resistance to fluoroquinolones observed in the present study is crucial in view of fluoroquinolones being drugs of choice for the treatment of human <em>Campylobacter</em> infections.</p></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0362028X24000929/pdfft?md5=53c372f133a6052ef97eaf02c7b11769&pid=1-s2.0-S0362028X24000929-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Short Variable Regions flaA Gene (SVR-flaA) Diversity and Virulence Profile of Multidrug−Resistant Campylobacter from Poultry and Poultry Meat in India\",\"authors\":\"Saima Iqbal , Sabia Qureshi , Muddasir S. Banday , Shaheen Farooq , Zahid A. Kashoo , Maliha Gulzar , M. Altaf Bhat , Arif Pandit , Md. Isfaqul Hussain , Pervaiz Dar , Gulzar Badroo , Mahrukh Hafiz , Faheem ud Din , Junaid Mehraj\",\"doi\":\"10.1016/j.jfp.2024.100308\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Human gastrointestinal infections caused by <em>Campylobacter</em> species is the second most important foodborne illness after salmonellosis worldwide. Poultry represent one of the main sources of <em>Campylobacter</em> organisms. In the present study, the short variable region of flagellin gene (SVR-<em>fla</em>A) typing was carried out to determine the variation among the circulating strains of <em>Campylobacter jejuni</em> and <em>Campylobacter coli</em>. The <em>C. jejuni</em> and <em>C. coli</em> isolated from poultry and poultry meat were screened for the presence of virulence determinants like <em>cad</em>F, <em>fla</em>A, <em>cdt</em>B, and <em>wla</em>N gene. The screening for <em>wla</em>N gene is crucial in view of the fact that most patients with Guillian Barre’s (GB) syndrome with a preceding history of diarrheal illness have been found to harbor <em>wla</em>N gene−positive <em>C jejuni</em> strains. Out of the 200 samples comprising poultry meat and cloacal swabs, 21.5% of samples were found to harbor <em>Campylobacter</em> spp. of which 2.5% were <em>Campylobacter jejuni</em>, and 19% were confirmed as <em>Campylobacter coli</em>. The <em>cad</em>F, <em>fla</em>A, <em>cdt</em>B virulence genes were detected in all the <em>Campylobacter</em> spp. isolated in the present study. The presence of the <em>wla</em>N gene in the <em>Campylobacter jejuni</em> isolated in the present study may pose a public health threat with long−term human health implications. The SVR-<em>fla</em>A typing of twelve <em>Campylobacter</em> isolates obtained in the present study revealed that <em>Campylobacter coli fla</em>A sequence OL471375 is a new strain with a novel allele type 1,675 and peptide sequence 5 which stands deposited in pubMLST database for <em>Campylobacter</em>. The other <em>fla</em>A-SVR gene sequences identified in this study were OL471369, OL471370, OL471371, OL471372, OL471373, and OL471374. Among twelve <em>Campylobacter</em> spp., three distinct <em>Dde</em>I-RFLP patterns were observed, each varying in size from 100 to 1,000 base pairs. Antimicrobial profiling of the <em>Campylobacter</em> spp. isolated in the present study revealed that 50% of the strains were multidrug resistant. All the <em>Campylobacter</em> spp. were resistant to ciprofloxacin (CIP), ampicillin (AMP), penicillin (PEN), and nalidixic acid (NAL) whereas 57.1% of strains were resistant to tetracycline (TET) and erythromycin (ERY) 28% to amoxicillin (AMX) and enrofloxacin (ENO), 85% to amikacin (AMK). The high degree of resistance to fluoroquinolones observed in the present study is crucial in view of fluoroquinolones being drugs of choice for the treatment of human <em>Campylobacter</em> infections.</p></div>\",\"PeriodicalId\":15903,\"journal\":{\"name\":\"Journal of food protection\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0362028X24000929/pdfft?md5=53c372f133a6052ef97eaf02c7b11769&pid=1-s2.0-S0362028X24000929-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of food protection\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0362028X24000929\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of food protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0362028X24000929","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Short Variable Regions flaA Gene (SVR-flaA) Diversity and Virulence Profile of Multidrug−Resistant Campylobacter from Poultry and Poultry Meat in India
Human gastrointestinal infections caused by Campylobacter species is the second most important foodborne illness after salmonellosis worldwide. Poultry represent one of the main sources of Campylobacter organisms. In the present study, the short variable region of flagellin gene (SVR-flaA) typing was carried out to determine the variation among the circulating strains of Campylobacter jejuni and Campylobacter coli. The C. jejuni and C. coli isolated from poultry and poultry meat were screened for the presence of virulence determinants like cadF, flaA, cdtB, and wlaN gene. The screening for wlaN gene is crucial in view of the fact that most patients with Guillian Barre’s (GB) syndrome with a preceding history of diarrheal illness have been found to harbor wlaN gene−positive C jejuni strains. Out of the 200 samples comprising poultry meat and cloacal swabs, 21.5% of samples were found to harbor Campylobacter spp. of which 2.5% were Campylobacter jejuni, and 19% were confirmed as Campylobacter coli. The cadF, flaA, cdtB virulence genes were detected in all the Campylobacter spp. isolated in the present study. The presence of the wlaN gene in the Campylobacter jejuni isolated in the present study may pose a public health threat with long−term human health implications. The SVR-flaA typing of twelve Campylobacter isolates obtained in the present study revealed that Campylobacter coli flaA sequence OL471375 is a new strain with a novel allele type 1,675 and peptide sequence 5 which stands deposited in pubMLST database for Campylobacter. The other flaA-SVR gene sequences identified in this study were OL471369, OL471370, OL471371, OL471372, OL471373, and OL471374. Among twelve Campylobacter spp., three distinct DdeI-RFLP patterns were observed, each varying in size from 100 to 1,000 base pairs. Antimicrobial profiling of the Campylobacter spp. isolated in the present study revealed that 50% of the strains were multidrug resistant. All the Campylobacter spp. were resistant to ciprofloxacin (CIP), ampicillin (AMP), penicillin (PEN), and nalidixic acid (NAL) whereas 57.1% of strains were resistant to tetracycline (TET) and erythromycin (ERY) 28% to amoxicillin (AMX) and enrofloxacin (ENO), 85% to amikacin (AMK). The high degree of resistance to fluoroquinolones observed in the present study is crucial in view of fluoroquinolones being drugs of choice for the treatment of human Campylobacter infections.
期刊介绍:
The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with:
Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain;
Microbiological food quality and traditional/novel methods to assay microbiological food quality;
Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation;
Food fermentations and food-related probiotics;
Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers;
Risk assessments for food-related hazards;
Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods;
Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.