利用生物多样性调查采集的野生蜜蜂的微生物组和花卉关联。

IF 4.3 2区 生物学 Q2 MICROBIOLOGY
Phuong N. Nguyen, Farida Samad-zada, Katherine D. Chau, Sandra M. Rehan
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引用次数: 0

摘要

蜜蜂的健康状况可以通过其微生物组来评估,微生物组是一种生物标志物,表明蜜蜂群落中存在有益和有害微生物。本研究介绍了双色汗蜂(Agapostemon virescens)成蜂角质层上细菌、真菌和植物组成的特征。研究人员在双色汗蜂栖息地的北部范围内使用了各种方法收集这些蜜蜂,如盘式诱捕器、蓝色叶片诱捕器和扫网。研究人员采用非破坏性方法从这些野生蜜蜂的整个针状标本中提取 DNA。然后对 16S rRNA、ITS 和 rbcL 区域进行元标码。研究发现,采集方法会影响某些微生物和植物类群的检测。在各种采集方法中,扫网样本的真菌阿尔法多样性最低。然而,细菌或真菌贝塔多样性的微小差异表明,没有一种方法明显优于其他方法。因此,多种技术的结合可以满足更广泛的微生物检测需要。研究还揭示了细菌、真菌和植物多样性的地区差异。A. virescens的核心微生物组包括两种细菌、三种真菌和一种植物协会,所有这些都是在其他野生蜜蜂中经常检测到的。这些核心微生物在不同的采集方法和采集地点保持一致。对不同物种和地貌的野生蜜蜂微生物组的进一步广泛研究将有助于发现授粉者健康与其环境之间的重要关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microbiome and floral associations of a wild bee using biodiversity survey collections

Microbiome and floral associations of a wild bee using biodiversity survey collections

Microbiome and floral associations of a wild bee using biodiversity survey collections

The health of bees can be assessed through their microbiome, which serves as a biomarker indicating the presence of both beneficial and harmful microorganisms within a bee community. This study presents the characterisation of the bacterial, fungal, and plant composition on the cuticle of adult bicoloured sweat bees (Agapostemon virescens). These bees were collected using various methods such as pan traps, blue vane traps and sweep netting across the northern extent of their habitat range. Non-destructive methods were employed to extract DNA from the whole pinned specimens of these wild bees. Metabarcoding of the 16S rRNA, ITS and rbcL regions was then performed. The study found that the method of collection influenced the detection of certain microbial and plant taxa. Among the collection methods, sweep net samples showed the lowest fungal alpha diversity. However, minor differences in bacterial or fungal beta diversity suggest that no single method is significantly superior to others. Therefore, a combination of techniques can cater to a broader spectrum of microbial detection. The study also revealed regional variations in bacterial, fungal and plant diversity. The core microbiome of A. virescens comprises two bacteria, three fungi and a plant association, all of which are commonly detected in other wild bees. These core microbes remained consistent across different collection methods and locations. Further extensive studies of wild bee microbiomes across various species and landscapes will help uncover crucial relationships between pollinator health and their environment.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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