从卡廷加生物群落的兽医院设施和粪便中分离出的葡萄球菌的抗菌药耐药性、肠毒素和生物膜生成基因。

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-31 DOI:10.1007/s42770-024-01400-3
José Diniz de Souto Sobrinho, Ana Karolione de Valença Silva, Katianny Bezerra de Medeiros, Maria Luana Cristiny Rodrigues Silva, Ana Beatriz Monteiro de Medeiros, Débora Luise Canuto de Sousa, Sérgio Santos de Azevedo, Carolina de Sousa Américo Batista Santos
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引用次数: 0

摘要

卡廷加生物群落仅出现在巴西,其流行病学条件应与巴西和世界其他地区的流行病学条件进行不同的评估。因此,本次调查的目的是鉴定从卡廷加生物群落一家兽医院的设施和粪便中分离出来的葡萄球菌的抗菌药耐药性、肠毒素和生物膜生成基因。使用无菌拭子从小动物临床护理台(n = 8)、猫狗住院部笼子和患有传染病的动物(n = 21)、小动物外科中心(n = 8)、消毒部门(n = 7)和听诊器(n = 32)的表面采集样本。进行了细菌分离和鉴定、抗菌素耐药性表型测试以及抗菌素耐药性、生物膜形成和肠毒素基因的分子检测。总体而言,6 种葡萄球菌中有 13 种(44.8%)表现出抗菌药耐药性,其中溶血性葡萄球菌表现出多药耐药性表型。耐药率最高的抗菌素是青霉素和四环素。最常见的耐药基因是 blaZ 和 tetM,在 10 个(76.9%)分离株中都检测到了这两种基因。mecA、tetL 和 tetK 基因的频率分别为 38.5%(5/13)、23.1%(3/13)和 15.4%(2/13)。在一株 S. sciuri 菌株中检测到了生物膜生成标记 icaD 基因。在 15.4%(2/13)的菌株(假中间葡萄球菌和中间葡萄球菌)中检测到编码肠毒素的 SEE 基因。在卡廷加生物群落的兽医院设施和粪便中发现了对不同类别的抗菌素具有耐药性基因的葡萄球菌,这些葡萄球菌具有 MDR 表型模式,并携带肠毒素和编码生物菲姆的基因,因此有必要在兽医实践中实施预防措施,以避免出现影响 "一个健康 "的情况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial resistance, enterotoxin and biofilm production genes in Staphylococcus spp. isolated from facilities and fomites in veterinary hospital in the Caatinga biome.

The Caatinga biome occurs only in Brazil and offers epidemiological conditions that should be assessed differently from other regions of Brazil and the world. Thus, the aim of this survey was to identify antimicrobial resistance, enterotoxin and biofilm production genes in Staphylococcus spp. isolated from facilities and fomites in a veterinary hospital in Caatinga biome. Samples were collected from surfaces of small animal clinical care tables (n =8), cages in the dog and cat hospitalisation sector and animals with infectious diseases (n = 21), small animal surgical centre (n =8), sterilisation sector (n =7) and stethoscopes (n = 32) by using sterile swabs. Bacterial isolation and identification, antimicrobial resistance phenotypic test and molecular detection of antimicrobial resistance, biofilm formation and enterotoxin genes were carried out. Ninety-five bacterial isolates were obtained, and 29 (30.5%) were identified as Staphylococcus spp. Overall, 13 isolates (44.8%) of six species of Staphylococcus spp. showed antimicrobial resistance profile, as well as S. haemolyticus expressed phenotypic profile of multidrug resistance. The antimicrobials with the highest resistance rates were penicillin and tetracycline. The most frequent resistance genes were blaZ and tetM, both detected in 10 (76.9%) isolates. The mecA, tetL and tetK genes had frequencies of 38.5% (5/13), 23.1% (3/13) and 15.4% (2/13), respectively. The biofilm production marker, icaD gene, was detected in one S. sciuri strain. SEE gene, which encodes enterotoxins, was detected in 15.4% (2/13) of the strains (S. pseudintermedius and S. intermedius). The occurrence of Staphylococcus spp. carrying resistance genes to diferent classes of antimicrobials, presenting MDR phenotypic pattern and carrying enterotoxins and biofim encoding genes recovered from veterinary hospital facilities and fomites in the Caatinga biome reinforce the need to implement prevention cares in veterinary practices to avoid One Health-concerning conditions.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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