Yongxin Zhang, Beian Liu, Meina Yang, Shixu Li, Yunhao Qu, Yingge Ma, Lin Ye, Jun Mei
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The flora of samples was analysed with 16S rRNA sequencing, and the data was analysed using QIIME, R, LefSE and other software. The potential functions were analysed by PICRUSt.</p><p><strong>Results: </strong>A total of 1440 operational taxonomic units (OTUs) were obtained, 139 specific to group A, 220 specific to group B, and 239 specific to group C. There was no significant difference in α index between the three groups. The beta diversity and grouping analysis data indicated that the three groups of flora were similar in species richness and diversity, but there were some differences in composition. In group A, the abundance of <i>Pseudomonadaceae, Chlorobacteria, Moraceae, Staphylococcaceae, Bacillariophyceae, Immunobacterium spp</i>. and <i>Bacillus spp</i>. was higher; in group B, the abundance of <i>Burkholderiaceae, Sphingomonas, Rhizobia, Stalked Bacteria, Sphingomonadaceae, Enterobacteriaceae, Shortwaveomonas spp</i>. was higher; in group C, the abundance of <i>Streptococcus digestiveis, Propionibacterium, Enterobacteriaceae, Anaerobacteriaceae, Propionibacteriaceae, Bacillus spp. Neisseria spp</i>. and <i>Shortactomonas spp</i>. was higher. Six pathways were identified to assess the potential microbial functions.</p><p><strong>Conclusion: </strong>Alterations in the microbiota of the conjunctiva and lacrimal sac are associated with the pathogenesis of CDC, which may provide certain guidance for antibiotic treatment of CDC.</p>","PeriodicalId":10214,"journal":{"name":"Clinical and Experimental Optometry","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"16S rRNA sequencing in chronic dacryocystitis.\",\"authors\":\"Yongxin Zhang, Beian Liu, Meina Yang, Shixu Li, Yunhao Qu, Yingge Ma, Lin Ye, Jun Mei\",\"doi\":\"10.1080/08164622.2024.2358246\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Clinical relevance: </strong>The pathogenesis of chronic dacryocystitis (CDC) is associated with a variety of bacteria. 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引用次数: 0
摘要
临床意义：慢性泪囊炎（CDC）的发病机制与多种细菌有关。研究微生物菌群有可能为预防和治疗 CDC 提供理论依据。背景：16S rRNA 测序是一种基于序列的细菌分析方法。16S rRNA 测序在 CDC 中的应用鲜有报道：方法：对被诊断为 CDC 患者的感染眼和健康眼进行病例对照研究。78 名患者被分为 A 组（来自健康眼睛的结膜囊分泌物）、B 组（来自患眼的结膜囊分泌物）和 C 组（来自患眼的泪囊分泌物）。采用 16S rRNA 测序分析样本菌群，并使用 QIIME、R、LefSE 等软件分析数据。结果：结果：共获得 1440 个操作分类单元（OTUs），其中 A 组 139 个，B 组 220 个，C 组 239 个。贝塔多样性和分组分析数据表明，三组植物区系的物种丰富度和多样性相似，但组成存在一定差异。在 A 组中，假单胞菌科（Pseudomonadaceae）、氯细菌科（Chlorobacteria）、大戟科（Moraceae）、葡萄球菌科（Staphylococcaceae）、芽孢杆菌科（Bacillariophyceae）、免疫杆菌属（Immunobacterium spp.在 C 组中，消化链球菌、丙酸杆菌、肠杆菌科、厌氧菌科、丙酸杆菌科、芽孢杆菌属、奈瑟氏菌属和短波单胞菌属的数量较多。为评估潜在的微生物功能，确定了六种途径：结膜和泪囊微生物群的改变与 CDC 的发病机制有关，这可能为 CDC 的抗生素治疗提供一定的指导。
Clinical relevance: The pathogenesis of chronic dacryocystitis (CDC) is associated with a variety of bacteria. Investigating microflora has the potential to provide a theoretical basis for preventing and treating CDC.
Background: 16S rRNA sequencing is a sequence-based bacterial analysis. The application of 16S rRNA sequencing in CDC is rarely reported.
Methods: A case-control study of infected and healthy eyes diagnosed as CDC patients was conducted. Seventy-eight patients were divided into A (conjunctival sac secretions from healthy eyes), B (conjunctival sac secretions from affected eyes), and C (lacrimal sac secretions from affected eyes) groups. The flora of samples was analysed with 16S rRNA sequencing, and the data was analysed using QIIME, R, LefSE and other software. The potential functions were analysed by PICRUSt.
Results: A total of 1440 operational taxonomic units (OTUs) were obtained, 139 specific to group A, 220 specific to group B, and 239 specific to group C. There was no significant difference in α index between the three groups. The beta diversity and grouping analysis data indicated that the three groups of flora were similar in species richness and diversity, but there were some differences in composition. In group A, the abundance of Pseudomonadaceae, Chlorobacteria, Moraceae, Staphylococcaceae, Bacillariophyceae, Immunobacterium spp. and Bacillus spp. was higher; in group B, the abundance of Burkholderiaceae, Sphingomonas, Rhizobia, Stalked Bacteria, Sphingomonadaceae, Enterobacteriaceae, Shortwaveomonas spp. was higher; in group C, the abundance of Streptococcus digestiveis, Propionibacterium, Enterobacteriaceae, Anaerobacteriaceae, Propionibacteriaceae, Bacillus spp. Neisseria spp. and Shortactomonas spp. was higher. Six pathways were identified to assess the potential microbial functions.
Conclusion: Alterations in the microbiota of the conjunctiva and lacrimal sac are associated with the pathogenesis of CDC, which may provide certain guidance for antibiotic treatment of CDC.
期刊介绍：
Clinical and Experimental Optometry is a peer reviewed journal listed by ISI and abstracted by PubMed, Web of Science, Scopus, Science Citation Index and Current Contents. It publishes original research papers and reviews in clinical optometry and vision science. Debate and discussion of controversial scientific and clinical issues is encouraged and letters to the Editor and short communications expressing points of view on matters within the Journal''s areas of interest are welcome. The Journal is published six times annually.
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