Aleksander Roszczyk, Michał Zych, Dariusz Sołdacki, Radoslaw Zagozdzon, Monika J Kniotek
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引用次数: 0
摘要
流式细胞仪方法可以帮助医生对免疫缺陷患者进行诊断和治疗监测。大多数临床建议都侧重于寻找 T 淋巴细胞和 B 淋巴细胞亚群的变化,自然杀伤(NK)细胞和粒细胞的变化则不太常见。虽然许多国家都公布了具有临床意义的淋巴细胞亚群的参考值,但我们还没有找到波兰成年居民的重要数据;因此,我们根据性别和年龄确定了 T、B 和 NK 亚群的参考值。与男性相比,女性组的淋巴细胞(CD45++)、T 辅助淋巴细胞(绝对计数较高)、CD4/CD8 比率、边缘区样 B 细胞、类调换 B 细胞和 CD21 低 B 细胞的百分比较高。男性组的幼稚 B 淋巴细胞、过渡性 B 细胞和浆细胞的百分比也较高。双阳性 T 淋巴细胞、自然杀伤 T 细胞(NKT)淋巴细胞和 CD21low B 细胞与年龄呈弱正相关。双阴性 T 淋巴细胞、边缘区样 B 细胞和浆细胞与年龄呈负相关。结果表明,就免疫表型而言,建立不同性别和年龄的参考范围非常重要。
Reference values of lymphocyte subsets from healthy Polish adults.
The flow cytometry method could support physicians' decisions in the diagnosis and treatment monitoring of immunodeficient patients. Most clinical recommendations are focused on the search for alterations in T- and B-lymphocyte subsets, less commonly natural killer (NK) cells and granulocytes. While reference values for clinically meaningful lymphocyte subsets have been published ubiquitously among numerous countries, we have not found significant data for a population of adult Polish habitats; thus we determined reference values for T, B, and NK subsets according to sex and age. The female group showed a higher percentage of lymphocytes (CD45++), T helper lymphocytes with a higher absolute count, as well as CD4/CD8 ratio, marginal zone-like B cells, class-switched B cells, and CD21low B cells than the male group. The male group was found to have elevated percentages of naïve B lymphocytes, transitional B cells, and plasmablasts. A weak positive correlation with age was found among double positive T lymphocytes, natural killer T cells (NKT) lymphocytes, and CD21low B cells. A negative correlation with age for double negative T lymphocytes, marginal zone-like B cells, and plasmablasts was noted. The results indicated the importance of creating distinct reference ranges regarding sex and age concerning immunophenotype.