Saptaparna Dutta, Sankalan Dey, Anindita Chakraborty, Sarmistha Sen Raychaudhuri
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引用次数: 0
摘要
伽马辐射(GR)和水杨酸(SA)通过调节植物的生长和发育来提高植物的性状。然而,人们对它们在植物中的综合疗法的基本机制还不甚了解。在我们的研究中,我们评估了 100 μM 和 500 μM SA 和/或 20、40 Gy 对药用作物 Momordica charantia 的潜在影响。我们观察到,在 20 Gy 的剂量下,植物根-芽长度和光合效率明显增加,而在施用 SA 的剂量下,植物根-芽长度和光合效率进一步提高。总之,同时施用 SA 和 GR 还能通过产生次生代谢物(如类黄酮和酚类物质)提高抗氧化能力。在 20 Gy + 500 μM SA 组合中观察到最高的抗糖尿病活性,对α-淀粉酶的抑制率高达 81.5%。在 GR 和/或 SA 的作用下,参与β-谷甾醇生物合成的关键基因--甾醇 C-22 去饱和酶的表达上调。β-谷甾醇是一种重要的化合物,具有很强的抗胆固醇和抗致癌性。我们的研究表明,在接受 20 Gy + 500 μM SA 联合处理的 M. charantia 植物中,β-谷甾醇的含量最高(1082.2 ± 68.2 ng/mg FW)。因此,伽马辐照和水杨酸有助于木香的可持续生长和各种次生代谢产物的产生,从而提高其农艺价值。
Exogenous application of salicylic acid and low-dose ionizing radiation increases synthesis of bioactive compounds and upregulates phytosterol production of Bitter melon (Momordica charantia)
Gamma radiation (GR) and salicylic acid (SA) enhance plant traits by regulating growth and development. However, the underlying mechanism of their combined therapies in plants is not well understood. In our study, the potential impact of 100 μM and 500 μM SA and/or 20, 40 Gy in Momordica charantia, a medicinal crop plant was assessed. We observed significant increase in plant root-shoot length and photosynthetic efficiency at 20 Gy dose, which further enhanced under SA application. Overall, simultaneous application of SA and GR also upregulated antioxidant capacity via production of secondary metabolites, such as flavonoids and phenolics. The highest antidiabetic activity with up to 81.5% inhibition of alpha-amylase enzyme was observed at 20 Gy + 500 μM SA combination. The expression of Sterol C-22 Desaturase, a key gene involved in the biosynthesis of β-sitosterol was upregulated under the treatment of GR and/or SA. β-sitosterol is an important compound with potent anti-cholesterol and anti-carcinogenic properties. Our study showed that highest content of β-sitosterol (1082.2 ± 68.2 ng/mg FW) was observed in M. charantia plants exposed to combined 20 Gy + 500 μM SA treatment. Therefore, gamma irradiation and salicylic acid can help in sustainable growth and production of various secondary metabolites in M. charantia, thereby enhancing its agronomic value.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.