Jing Zhao, Zixuan Li, Changzhong Li, Rong Wang, Zifeng Zhao, Huayu Gou, Lanying Li, Anbin Xie, Haotian Ren, Bo Qiu, Xiaodie Li, Yanxia Chen, Zhenji Wang, Guojie Wang, Wenjie Jin
{"title":"Gymnocypris eckloni 在铜和铅离子挑战下对 GeCu/Zn-SOD、GeMn-SOD、GeHsp90 和 GeMT 的调控","authors":"Jing Zhao, Zixuan Li, Changzhong Li, Rong Wang, Zifeng Zhao, Huayu Gou, Lanying Li, Anbin Xie, Haotian Ren, Bo Qiu, Xiaodie Li, Yanxia Chen, Zhenji Wang, Guojie Wang, Wenjie Jin","doi":"10.1155/2024/5716920","DOIUrl":null,"url":null,"abstract":"<p>The contamination of aquatic environments by heavy metals can have detrimental effects on fish, impacting their growth and overall health, including the regulation of antioxidant genes. An investigation was carried out to assess the distribution and habitat of <i>Gymnocypris eckloni</i> in the Yellow River basin. Simultaneously, heavy metal concentrations in its habitat and in selected locations within the upper Yellow River were measured. In an effort to explore the potential roles of specific genes in antioxidant responses, <i>G. eckloni</i> was exposed to low concentrations of copper (Cu<sup>2+</sup>) and lead (Pb<sup>2+</sup>) for varying durations (12, 24, and 48 hours). The mRNA levels of <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, <i>GeHsp90</i>, and <i>GeMT</i> were quantified in the gills, kidneys, and liver through qRT-PCR. The findings suggest that the habitat of <i>G. eckloni</i> is generally safe; however, occasional exceedances of safety standards could pose a potential threat to its growth. Importantly, the expression of <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, <i>GeHsp90</i>, and <i>GeMT</i> exhibited responses to the low concentrations of copper-induced and lead-induced stress. Notably, <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, and <i>GeMT</i> demonstrated heightened sensitivity to lead compared to copper. Furthermore, the expression of these genes displayed tissue-specific responses under identical metal stress conditions. These results indicate that <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, <i>GeHsp90</i>, and <i>GeMT</i> genes have the potential to serve as early, sensitive biomarkers for the detection of metal toxicity induced by Cu<sup>2+</sup> and Pb<sup>2+</sup>. This study also provides valuable insights into the functioning of antioxidant genes under oxidative stress in fish.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Regulation of GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT in Gymnocypris eckloni in Response to Copper and Lead Ion Challenges\",\"authors\":\"Jing Zhao, Zixuan Li, Changzhong Li, Rong Wang, Zifeng Zhao, Huayu Gou, Lanying Li, Anbin Xie, Haotian Ren, Bo Qiu, Xiaodie Li, Yanxia Chen, Zhenji Wang, Guojie Wang, Wenjie Jin\",\"doi\":\"10.1155/2024/5716920\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The contamination of aquatic environments by heavy metals can have detrimental effects on fish, impacting their growth and overall health, including the regulation of antioxidant genes. An investigation was carried out to assess the distribution and habitat of <i>Gymnocypris eckloni</i> in the Yellow River basin. Simultaneously, heavy metal concentrations in its habitat and in selected locations within the upper Yellow River were measured. In an effort to explore the potential roles of specific genes in antioxidant responses, <i>G. eckloni</i> was exposed to low concentrations of copper (Cu<sup>2+</sup>) and lead (Pb<sup>2+</sup>) for varying durations (12, 24, and 48 hours). The mRNA levels of <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, <i>GeHsp90</i>, and <i>GeMT</i> were quantified in the gills, kidneys, and liver through qRT-PCR. The findings suggest that the habitat of <i>G. eckloni</i> is generally safe; however, occasional exceedances of safety standards could pose a potential threat to its growth. Importantly, the expression of <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, <i>GeHsp90</i>, and <i>GeMT</i> exhibited responses to the low concentrations of copper-induced and lead-induced stress. Notably, <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, and <i>GeMT</i> demonstrated heightened sensitivity to lead compared to copper. Furthermore, the expression of these genes displayed tissue-specific responses under identical metal stress conditions. These results indicate that <i>GeCu/Zn-SOD</i>, <i>GeMn-SOD</i>, <i>GeHsp90</i>, and <i>GeMT</i> genes have the potential to serve as early, sensitive biomarkers for the detection of metal toxicity induced by Cu<sup>2+</sup> and Pb<sup>2+</sup>. This study also provides valuable insights into the functioning of antioxidant genes under oxidative stress in fish.</p>\",\"PeriodicalId\":14894,\"journal\":{\"name\":\"Journal of Applied Ichthyology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2024-03-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Ichthyology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/2024/5716920\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Ichthyology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2024/5716920","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FISHERIES","Score":null,"Total":0}
Regulation of GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT in Gymnocypris eckloni in Response to Copper and Lead Ion Challenges
The contamination of aquatic environments by heavy metals can have detrimental effects on fish, impacting their growth and overall health, including the regulation of antioxidant genes. An investigation was carried out to assess the distribution and habitat of Gymnocypris eckloni in the Yellow River basin. Simultaneously, heavy metal concentrations in its habitat and in selected locations within the upper Yellow River were measured. In an effort to explore the potential roles of specific genes in antioxidant responses, G. eckloni was exposed to low concentrations of copper (Cu2+) and lead (Pb2+) for varying durations (12, 24, and 48 hours). The mRNA levels of GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT were quantified in the gills, kidneys, and liver through qRT-PCR. The findings suggest that the habitat of G. eckloni is generally safe; however, occasional exceedances of safety standards could pose a potential threat to its growth. Importantly, the expression of GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT exhibited responses to the low concentrations of copper-induced and lead-induced stress. Notably, GeCu/Zn-SOD, GeMn-SOD, and GeMT demonstrated heightened sensitivity to lead compared to copper. Furthermore, the expression of these genes displayed tissue-specific responses under identical metal stress conditions. These results indicate that GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT genes have the potential to serve as early, sensitive biomarkers for the detection of metal toxicity induced by Cu2+ and Pb2+. This study also provides valuable insights into the functioning of antioxidant genes under oxidative stress in fish.
期刊介绍:
The Journal of Applied Ichthyology publishes articles of international repute on ichthyology, aquaculture, and marine fisheries; ichthyopathology and ichthyoimmunology; environmental toxicology using fishes as test organisms; basic research on fishery management; and aspects of integrated coastal zone management in relation to fisheries and aquaculture. Emphasis is placed on the application of scientific research findings, while special consideration is given to ichthyological problems occurring in developing countries. Article formats include original articles, review articles, short communications and technical reports.