Gymnocypris eckloni 在铜和铅离子挑战下对 GeCu/Zn-SOD、GeMn-SOD、GeHsp90 和 GeMT 的调控

IF 0.7 4区 农林科学 Q4 FISHERIES
Jing Zhao, Zixuan Li, Changzhong Li, Rong Wang, Zifeng Zhao, Huayu Gou, Lanying Li, Anbin Xie, Haotian Ren, Bo Qiu, Xiaodie Li, Yanxia Chen, Zhenji Wang, Guojie Wang, Wenjie Jin
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引用次数: 0

摘要

重金属对水生环境的污染会对鱼类产生有害影响,影响其生长和整体健康,包括对抗氧化基因的调控。研究人员对黄河流域鲤科鱼类(Gymnocypris eckloni)的分布和栖息地进行了调查。同时,对其栖息地和黄河上游选定地点的重金属浓度进行了测量。为了探索特定基因在抗氧化反应中的潜在作用,Gymnocypris eckloni暴露于低浓度铜(Cu2+)和铅(Pb2+)的时间长短不一(12、24和48小时)。通过 qRT-PCR 对鱼鳃、肾脏和肝脏中 GeCu/Zn-SOD、GeMn-SOD、GeHsp90 和 GeMT 的 mRNA 水平进行了定量分析。研究结果表明,G. eckloni 的栖息地总体上是安全的;但是,偶尔超过安全标准可能会对其生长构成潜在威胁。重要的是,GeCu/Zn-SOD、GeMn-SOD、GeHsp90 和 GeMT 的表达对低浓度铜诱导胁迫和铅诱导胁迫有反应。值得注意的是,与铜相比,GeCu/Zn-SOD、GeMn-SOD 和 GeMT 对铅的敏感性更高。此外,在相同的金属应激条件下,这些基因的表达显示出组织特异性反应。这些结果表明,GeCu/Zn-SOD、GeMn-SOD、GeHsp90 和 GeMT 基因有可能成为检测 Cu2+ 和 Pb2+ 诱导的金属毒性的早期灵敏生物标志物。这项研究还为了解鱼类在氧化应激下抗氧化基因的功能提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulation of GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT in Gymnocypris eckloni in Response to Copper and Lead Ion Challenges

The contamination of aquatic environments by heavy metals can have detrimental effects on fish, impacting their growth and overall health, including the regulation of antioxidant genes. An investigation was carried out to assess the distribution and habitat of Gymnocypris eckloni in the Yellow River basin. Simultaneously, heavy metal concentrations in its habitat and in selected locations within the upper Yellow River were measured. In an effort to explore the potential roles of specific genes in antioxidant responses, G. eckloni was exposed to low concentrations of copper (Cu2+) and lead (Pb2+) for varying durations (12, 24, and 48 hours). The mRNA levels of GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT were quantified in the gills, kidneys, and liver through qRT-PCR. The findings suggest that the habitat of G. eckloni is generally safe; however, occasional exceedances of safety standards could pose a potential threat to its growth. Importantly, the expression of GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT exhibited responses to the low concentrations of copper-induced and lead-induced stress. Notably, GeCu/Zn-SOD, GeMn-SOD, and GeMT demonstrated heightened sensitivity to lead compared to copper. Furthermore, the expression of these genes displayed tissue-specific responses under identical metal stress conditions. These results indicate that GeCu/Zn-SOD, GeMn-SOD, GeHsp90, and GeMT genes have the potential to serve as early, sensitive biomarkers for the detection of metal toxicity induced by Cu2+ and Pb2+. This study also provides valuable insights into the functioning of antioxidant genes under oxidative stress in fish.

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来源期刊
Journal of Applied Ichthyology
Journal of Applied Ichthyology 生物-海洋与淡水生物学
CiteScore
2.30
自引率
11.10%
发文量
73
审稿时长
3-8 weeks
期刊介绍: The Journal of Applied Ichthyology publishes articles of international repute on ichthyology, aquaculture, and marine fisheries; ichthyopathology and ichthyoimmunology; environmental toxicology using fishes as test organisms; basic research on fishery management; and aspects of integrated coastal zone management in relation to fisheries and aquaculture. Emphasis is placed on the application of scientific research findings, while special consideration is given to ichthyological problems occurring in developing countries. Article formats include original articles, review articles, short communications and technical reports.
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